LOCUS Exported 2729 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C27m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2729) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2729) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2729 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 88..106 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 110..153 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 156..177 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" CDS 193..816 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" primer_bind complement(266..285) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" misc_feature 828..848 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(854..871) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1025..1613) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1105..1124) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1784..2644) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2407..2426 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2645..20) /gene="bla" /label=AmpR promoter ORIGIN 1 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 61 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 121 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact 181 agtgggtctc aaatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg 241 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta 301 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc 361 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa 421 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga 481 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa 541 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct 601 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa 661 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta 721 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga 781 ttagaaaaac aacttaaatg tgaaagtggg tcctaaaggt agagacctac tagtagcggc 841 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 1021 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 1501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 1801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1921 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2401 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 2641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 2701 gatacatatt tgaatgtatt tagaaaaat //