LOCUS Exported 2831 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C32m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2831) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2831) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2831 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 739..759 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(765..782) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(936..1524) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1016..1035) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1695..2555) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2318..2337 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2556..2660) /gene="bla" /label=AmpR promoter primer_bind 2728..2746 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2750..2793 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2796..2817 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatgattgag aatacctata gcgaaaagtt cgagtccgcg ttcgaacaga tcaaggcggc 61 ggccaacgtg gatgccgcca tccgtattct ccaggcggaa tataacctcg atttcgtcac 121 ctaccatctc gcccagacga tcgcgagcaa gatcgatccg cccttcgtgc gcaccaccta 181 tccggatgcc tgggtttccc gctacctcct caacagctat gtgaaggtcg atccgatcgt 241 caagcagggc ttcgaacgcc agctgccctt cgactggagc gaggtcgaac cgacgccgga 301 ggcctatgcc atgctggtcg acgcccagaa acacggcatc ggtggcaatg gctactccat 361 ccccgtcgcc gacaaggcgc agcgccgcgc cctgctgtcg ctgaatgccc gtataccggc 421 cgacgaatgg accgagcttg tgcgccgctg ccgcaacgag tggatcgaga tcgcccatct 481 gatccaccgc aaggccgtct atgagctgca tggcgaaaac gatccggtgc cggcattgtc 541 gccgcgcgag atcgagtgtc tgcactggac cgccctcggc aaggattaca aggatatttc 601 ggtcatcctg ggcatatcag agcataccac acgcgattac ctgaagaccg cccgcttcaa 661 gctcggctgc gccacgatct cggccgccgc gtcgcgggct gttcaattgc gcatcatcaa 721 tccctgaagg tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg 781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac 961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 1021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 1081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 1141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 1201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 1261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 1321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 1381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 1441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 1501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 1561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 1621 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1681 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 1741 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 1801 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat 1861 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 1921 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 1981 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 2041 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 2101 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 2161 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 2221 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 2281 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 2341 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 2401 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 2461 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 2521 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 2581 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 2641 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 2701 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 2761 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact 2821 agtgggtctc a //