LOCUS       Exported                2860 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  MoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam 
            Meyer's improved CinR activator from https://doi.org/10.1101/285866,
            selected for dynamic range and less cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    C71m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2860)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(154..1014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     777..796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1015..1119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1187..1205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1209..1252
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1255..1276
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2058..2078
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2084..2101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2255..2843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2335..2354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
       61 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc
      301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     1201 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     1261 gcggccgctt ctagagacta gtgggtctca aatgattgag aatacctata gcgaaaagtt
     1321 cgagtccgcg ttcgaacaga tcaaagcggc ggccaacgtg gatgccgcca tccgtattct
     1381 ccaggcggaa tataacctcg atttcgtcac ctaccatctc gcccagacaa tcgcgagcaa
     1441 gatcgattcg cccttcgtgc gcaccaccta tccggatgcc tgggtttccc gttacctcct
     1501 caactgctat gtgaaggtcg atccgatcat caagcagggc ttcgaacgcc agctgccctt
     1561 cgactggagc gaggtcgaac cgacgccgga ggcctatgcc atgctggtcg acgcccagaa
     1621 acacggcatc gatgacaatg gctactccat ccccgtcgcc gacaaggcgc agcgccgcgc
     1681 cctgctgtcg ctgaatgccc atataccggc cgacgaatgg accgagctcg tgcgccgcta
     1741 ccgcaacgag tggatcgaga tcgcccatct gatccaccgc aaggccgtat atgagctgca
     1801 tggcgaaaac gatccggtgc cggcattgtc gccgcgcgag atcgagtgtc tgcactggac
     1861 cgccctcggc aaggattaca aggatatttc ggtcatcctg ggcatatcag agcataccac
     1921 acgcgattac ctgaaaaccg cccgcttcag gctcggctgc accacgatct cggccgccgc
     1981 gtcgcgggct gttcaattgt gcatcatcaa tccctatagg atccgcatga cgcgacgtaa
     2041 ttggtaaggt agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc
     2101 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
     2281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2701 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
//
