LOCUS Exported 2768 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C78m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2768) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2768) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2768 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 5..664 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(71..90) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" misc_feature 676..696 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(702..719) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(873..1461) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(953..972) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1632..2492) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2255..2274 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2493..2597) /gene="bla" /label=AmpR promoter primer_bind 2665..2683 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2687..2730 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2733..2754 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatgatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa 61 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct 121 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt 181 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga 241 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac 301 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat 361 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat 421 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa 481 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca 541 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt 601 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc 661 gtaaaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg 721 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 781 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 841 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag 901 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 961 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 1021 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 1081 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 1141 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 1201 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 1261 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 1321 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 1381 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 1441 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 1501 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 1561 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 1621 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 1681 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 1741 ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta 1801 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 1861 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 1921 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 1981 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 2041 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 2101 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 2161 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 2221 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 2281 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 2341 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 2401 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 2461 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 2521 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 2581 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 2641 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa 2701 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagt 2761 gggtctca //