LOCUS Exported 3746 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C90m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3746) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 3746) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3746 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(14..31) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(185..773) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(265..284) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(944..1804) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1567..1586 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1805..1909) /gene="bla" /label=AmpR promoter primer_bind 1977..1995 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1999..2042 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2045..2066 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" CDS 3006..3719 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" misc_feature 3734..8 /label=BioBrick suffix /note="universal suffix for all parts" ORIGIN 1 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 61 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 181 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1081 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 1261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 1321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 1381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 1441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 1501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 1561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 1621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 1681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 1741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 1801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 1861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 1921 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 1981 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 2041 tctggaattc gcggccgctt ctagagacta gtgggtctca aatggaactg cgtgacctgg 2101 atttaaacct gctggtggtg ttcaaccagt tgctggtcga cagacgcgtc tctgtcactg 2161 cggagaacct gggcctgacc cagcctgccg tgagcaatgc gctgaaacgc ctgcgcacct 2221 cgctacagga cccactcttc gtgcgcacac atcagggaat ggaacccaca ccctatgccg 2281 cgcatctggc cgagcacgtc acttcggcca tgcacgcact gcgcaacgcc ctacagcacc 2341 atgaaagctt cgatccgctg accagcgagc gtaccttcac cctggccatg accgacattg 2401 gcgagatcta cttcatgccg cggctgatgg atgcgctggc tcaccaggcc cccaattgcg 2461 tgatcagtac ggtgcgcgac agttcgatga gcctgatgca ggccttgcag aacggaaccg 2521 tggacttggc cgtgggcctg cttcccaatc tgcaaactgg cttctttcag cgccggctgc 2581 tccagaatca ctacgtgtgc ctatgtcgca aggaccatcc agtcacccgc gaacccctga 2641 ctctggagcg cttctgttcc tacggccacg tgcgtgtcat cgccgctggc accggccacg 2701 gcgaggtgga cacgtacatg acacgggtcg gcatccggcg cgacatccgt ctggaagtgc 2761 cgcacttcgc cgccgttggc cacatcctcc agcgcaccga tctgctcgcc actgtgccga 2821 tatgtttagc cgactgctgc gtagagccct tcggcctaag cgccttgccg cacccagtcg 2881 tcttgcctga aatagccatc aacatgttct ggcatgcgaa gtaccacaag gacctagcca 2941 atatttggtt gcggcaactg atgtttgacc tgtttacgga tggaagcgga agtggatccg 3001 gtagtatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg 3061 atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta 3121 atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga 3181 ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga 3241 agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt 3301 cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc 3361 tggtaaaccg cattgagctg aaaggcattg actttaaaga agatggcaat atcctgggcc 3421 ataagctgga atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa 3481 atggcattaa agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg 3541 ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc 3601 actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg 3661 ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat 3721 aaaggtagag acctactagt agcggc //