LOCUS Exported 2303 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C98m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2303) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2303) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2303 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 211..231 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(237..254) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(408..996) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(488..507) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1167..2027) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1790..1809 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2028..2132) /gene="bla" /label=AmpR promoter primer_bind 2200..2218 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2222..2265 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2268..2289 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatgatgtca aacgtaaata caggttcact tagtgtggac aataagaagt tttgggctac 61 cgtagagtcc tcggagcatt ccttcgaggt tccaatctac gctgagacac tagacgaagc 121 tctggagtta gccgaatggc aatacgttcc ggctggcttt gaggttactc gtgtgcgtcc 181 ttgtgtagca ccgaagtaaa ggtagagacc tactagtagc ggccgctgca ggcttcctcg 241 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 301 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 361 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc 421 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 601 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 841 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 1021 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 1081 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 1141 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 1201 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 1261 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgcga cccacgctca 1321 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 1381 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 1441 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 1501 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 1561 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 1621 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 1681 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 1741 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 1801 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 1861 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 1921 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 1981 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 2041 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 2101 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 2161 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag 2221 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg 2281 cttctagaga ctagtgggtc tca //