LOCUS Exported 2192 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).. ACCESSION . VERSION . KEYWORDS U3m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2192) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2192) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2192 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(156..260) /gene="bla" /label=AmpR promoter primer_bind 328..346 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 350..393 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 396..417 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 531..551 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(557..574) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(728..1316) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(808..827) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1487..155) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2110..2129 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 61 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 121 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 181 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 241 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 301 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 361 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact 421 agtgggtctc atactgggcc caagttcact taaaaaggag atcaacaatg aaagcaattt 481 tcgtactgaa acatcttaat catgcaatgg aggctttcta atgagagacc tactagtagc 541 ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 721 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 781 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 841 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 901 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 961 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 1021 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 1081 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 1141 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 1201 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 1261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 1321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 1381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 1441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 1501 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 1561 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 1621 taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 1681 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 1741 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 1801 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 1861 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1921 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 1981 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 2041 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 2101 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 2161 gttcttcggg gcgaaaactc tcaaggatct ta //