LOCUS       Exported               10787 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression of BRAF V600_K601delinsE.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-BRAF-V600_K601delinsE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3446..3455
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(5747..5771)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5779..5797
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5822..5841)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5823..5841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5847..5867)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5921..5962
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5983..6550
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6000..6552
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6144..6161)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6371..6390
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6564..7163
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6564..6583)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7060..7080
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7177..7765
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7230..7250)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7648..7659)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7840..8073
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8192..8210)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8278..8382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8383..9243
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8601..8620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9414..10002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9903..9922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10156..10173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10265..10284
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    10413..10434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10449..10479
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10487..10503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10492..10514
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10511..10527
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10511..10527
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10577..10596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcggcgctga gcggtggcgg tggtggcggc
     3481 gcggagccgg gccaggctct gttcaacggg gacatggagc ccgaggccgg cgccggcgcc
     3541 ggcgccgcgg cctcttcggc tgcggaccct gccattccgg aggaggtgtg gaatatcaaa
     3601 caaatgatta agttgacaca ggaacatata gaggccctat tggacaaatt tggtggggag
     3661 cataatccac catcaatata tctggaggcc tatgaagaat acaccagcaa gctagatgca
     3721 ctccaacaaa gagaacaaca gttattggaa tctctgggga acggaactga tttttctgtt
     3781 tctagctctg catcaatgga taccgttaca tcttcttcct cttctagcct ttcagtgcta
     3841 ccttcatctc tttcagtttt tcaaaatccc acagatgtgg cacggagcaa ccccaagtca
     3901 ccacaaaaac ctatcgttag agtcttcctg cccaacaaac agaggacagt ggtacctgca
     3961 aggtgtggag ttacagtccg agacagtcta aagaaagcac tgatgatgag aggtctaatc
     4021 ccagagtgct gtgctgttta cagaattcag gatggagaga agaaaccaat tggttgggac
     4081 actgatattt cctggcttac tggagaagaa ttgcatgtgg aagtgttgga gaatgttcca
     4141 cttacaacac acaactttgt acgaaaaacg tttttcacct tagcattttg tgacttttgt
     4201 cgaaagctgc ttttccaggg tttccgctgt caaacatgtg gttataaatt tcaccagcgt
     4261 tgtagtacag aagttccact gatgtgtgtt aattatgacc aacttgattt gctgtttgtc
     4321 tccaagttct ttgaacacca cccaatacca caggaagagg cgtccttagc agagactgcc
     4381 ctaacatctg gatcatcccc ttccgcaccc gcctcggact ctattgggcc ccaaattctc
     4441 accagtccgt ctccttcaaa atccattcca attccacagc ccttccgacc agcagatgaa
     4501 gatcatcgaa atcaatttgg gcaacgagac cgatcctcat cagctcccaa tgtgcatata
     4561 aacacaatag aacctgtcaa tattgatgac ttgattagag accaaggatt tcgtggtgat
     4621 ggaggatcaa ccacaggttt gtctgctacc ccccctgcct cattacctgg ctcactaact
     4681 aacgtgaaag ccttacagaa atctccagga cctcagcgag aaaggaagtc atcttcatcc
     4741 tcagaagaca ggaatcgaat gaaaacactt ggtagacggg actcgagtga tgattgggag
     4801 attcctgatg ggcagattac agtgggacaa agaattggat ctggatcatt tggaacagtc
     4861 tacaagggaa agtggcatgg tgatgtggca gtgaaaatgt tgaatgtgac agcacctaca
     4921 cctcagcagt tacaagcctt caaaaatgaa gtaggagtac tcaggaaaac acgacatgtg
     4981 aatatcctac tcttcatggg ctattccaca aagccacaac tggctattgt tacccagtgg
     5041 tgtgagggct ccagcttgta tcaccatctc catatcattg agaccaaatt tgagatgatc
     5101 aaacttatag atattgcacg acagactgca cagggcatgg attacttaca cgccaagtca
     5161 atcatccaca gagacctcaa gagtaataat atatttcttc atgaagacct cacagtaaaa
     5221 ataggtgatt ttggtctagc tacagaatct cgatggagtg ggtcccatca gtttgaacag
     5281 ttgtctggat ccattttgtg gatggcacca gaagtcatca gaatgcaaga taaaaatcca
     5341 tacagctttc agtcagatgt atatgcattt ggaattgttc tgtatgaatt gatgactgga
     5401 cagttacctt attcaaacat caacaacagg gaccagataa tttttatggt gggacgagga
     5461 tacctgtctc cagatctcag taaggtacgg agtaactgtc caaaagccat gaagagatta
     5521 atggcagagt gcctcaaaaa gaaaagagat gagagaccac tctttcccca aattctcgcc
     5581 tctattgagc tgctggcccg ctcattgcca aaaattcacc gcagtgcatc agaaccctcc
     5641 ttgaatcggg ctggtttcca aacagaggat tttagtctat atgcttgtgc ttctccaaaa
     5701 acacccatcc aggcaggggg atatggtgcg tttcctgtcc actagaaccc agctttcttg
     5761 tacaaagtgg tgatatccaa ttaaccctca ctaaaggtat tgttgaatgt gtttaaagag
     5821 accctatagt gagtcgtatt accacccaac ttttctatac aaagtggttg atatccagca
     5881 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     5941 tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa
     6001 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     6061 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     6121 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     6181 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     6241 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     6301 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     6361 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     6421 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     6481 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     6541 acgatgataa tatggccaca catatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6601 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6661 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6721 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6781 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6841 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6901 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6961 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     7021 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     7081 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     7141 tgacccgcaa gcccggtgcc taaatcgata gatcctaatc aacctctgga ttacaaaatt
     7201 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7261 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7321 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7381 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7441 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7501 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7561 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7621 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7681 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7741 atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag
     7801 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7861 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7921 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7981 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8041 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     8101 agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg
     8161 aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     8221 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     8281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8401 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8461 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8521 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8581 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     8641 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8701 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8761 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8821 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8881 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8941 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9001 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9061 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9121 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9181 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9541 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10021 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10081 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    10141 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    10201 ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt
    10261 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10321 gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc
    10381 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    10441 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    10501 caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt
    10561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    10621 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    10681 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    10741 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
