LOCUS       Exported                 802 bp ds-DNA     linear   SYN 19-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    c-myc promoter (4x TBE2 - wt)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 802)
  AUTHORS   He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin 
            PJ, Vogelstein B, Kinzler KW
  TITLE     Identification of c-MYC as a target of the APC pathway.
  JOURNAL   Science. 1998 Sep 4. 281(5382):1509-12.
  PUBMED    9727977
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      48..57
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    267..358
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(299..318)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    372..420
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(688..709)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cntcncggan ganggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt
       61 accaacagta ccggaatgcc aagcttactt agatcgcaga tctcgatacc ggatccatta
      121 tatacccgaa ttcgatatcg acgtccagct gtcgagcccg ggctagcttt gatcaagagt
      181 cctttgatca agagtccgct agctttgatc aagagtcctt tgatcaagag tccgctagca
      241 cgcgtaagag ctcggtacct atcgatagag aaatgttctg gcacctgcac ttgcactggg
      301 gacagcctat tttgctagtt tgttttgttt cgttttgttt tgatggagag cgtatgttag
      361 tactatcgat tcacacaaaa aaccaacaca cagatgtaat gaaaataaag atattttatt
      421 gcggccgctc caagtacctc ccgtacctta atattactta cttatcatgg tagcttgggc
      481 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      541 ggcgaatggc aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      601 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      661 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      721 gtggactcca acgtcaaagg gcgaaaaccg tctatcaggg cgatggccca ctacgtgaac
      781 catcacccta atcangtttg ga
//
