LOCUS Exported 7398 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Cadherin, Excitation: 568, Emission: 592. ACCESSION . VERSION . KEYWORDS mApple-Desmocollin1-C-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7398) TITLE Davidson lab - mApple constructs REFERENCE 2 (bases 1 to 7398) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7398 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 48..351 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 352..555 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 506..526 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 594..603 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 600..1307 /codon_start=1 /product="photostable monomeric derivative of DsRed (Shaner et al., 2008)" /label=mApple /note="mammalian codon-optimized" /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind 1013..1032 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 4173..4294 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4210..4229) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4264..4283 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4301..4756) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4438..4459) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4650..4669 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4783..4887 /gene="bla" /label=AmpR promoter promoter 4889..5246 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4912..4932) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 5097..5232 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5159..5178 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5281..6075 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5335..5354) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5945..5964 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(6263..6282) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 6307..6354 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6683..7271 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7172..7191 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 61 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 121 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 181 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 241 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 301 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg 361 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc 421 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa 481 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc 541 tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca 601 tggtgagcaa gggcgaggag aataacatgg ccatcatcaa ggagttcatg cgcttcaagg 661 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc 721 gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc cccctgccct 781 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggtctac attaagcacc 841 cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg tgggagcgcg 901 tgatgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc ctgcaggacg 961 gcgtgttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa 1021 tgcagaagaa gaccatgggc tgggaggcct ccgaggagcg gatgtacccc gaggacggcg 1081 ccctgaagag cgagatcaag aagaggctga agctgaagga cggcggccac tacgccgccg 1141 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg 1201 acatcaagtt ggacatcgtg tcccacaacg aggactacac catcgtggaa cagtacgaac 1261 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtcc ggactcagat 1321 ctagtagcgg aggtagcgca tccggcggaa gcggaggaag cgctctggcc tctgctgccc 1381 cagggagcat cttctgtaag cagctccttt tctctctcct ggttttaaca ttactttgcg 1441 atgcttgtca gaaagtttat cttcgagttc cttctcatct tcaggctgaa acacttgtag 1501 gcaaagtgaa tctggaggag tgtctcaagt cggccagcct aatccggtcc agtgaccctg 1561 ccttcaggat tctagaagat ggctcaattt acacaacaca tgacctcatt ttgtcttctg 1621 aaaggaaaag tttttccatt ttcctttcag atggtcagag acgggaacaa caagagataa 1681 aagttgtact gtcagcaaga gaaaacaagt ctcctaagaa gagacatacc aaagacacag 1741 ccctcaagcg cagcaagaga cgatgggctc ctattccagc ttcattgatg gagaactcgt 1801 tgggtccatt tccacaacac gttcagcaga tccaatctga tgctgcacag aattacacca 1861 tcttttattc cataagtggg ccaggcgtgg acaaagaacc cttcaatttg ttttacatag 1921 agaaagacac tggggatatc ttttgtacaa ggagcattga ccgtgagaaa tacgaacagt 1981 ttgcgttata tggctatgca acaactgcag atggctatgc accagaatat ccactccctt 2041 tgatcatcaa aattgaagat gataatgata acgccccata ttttgaacac agagtgacta 2101 tctttactgt gcctgaaaat tgccgatccg gcacttcagt gggaaaagtg accgccacag 2161 accttgacga acctgacact ctccatactc gtctgaaata taaaatctta caacaaatcc 2221 cagatcatcc aaagcatttc tccatacacc cagataccgg agtcatcacc acaactacac 2281 cttttctgga tagagaaaaa tgtgatactt accagttaat aatggaagtg cgagacatgg 2341 gtggtcagcc tttcggttta tttaatacag gaacaattac tatttcactt gaggatgaaa 2401 atgacaatcc accatctttc acagaaactt cttatgttac agaagtagaa gaaaacagaa 2461 ttgacgtgga gattttgcga atgaaggtac aggatcagga tttgccaaac actcctcact 2521 caaaggctgt atacaaaatc ttacaaggaa atgaaaatgg aaacttcata attagcacag 2581 atccaaatac aaatgaagga gtgctgtgtg ttgtcaagcc attgaactat gaagtcaatc 2641 gccaagttat tttgcaagtt ggtgtcatta acgaggcaca attctctaaa gcagcgagct 2701 cacaaactcc tacaatgtgc actacaactg tcaccgttaa aattatagac agtgatgagg 2761 gccctgaatg ccaccctcca gtgaaagtta ttcagagtca agatggcttc ccagctggcc 2821 aagaactcct tggatacaaa gcactggacc cggaaatatc cagtggtgaa ggcttaaggt 2881 atcagaagtt aggggatgaa gataactggt ttgaaattaa tcaacacact ggcgacttga 2941 gaactctaaa agtactagat agagaatcca aatttgtaaa aaacaaccaa tacaatattt 3001 cagttgttgc agtggatgca gttggccgat cttgcactgg aacattagta gttcatttgg 3061 atgattacaa cgatcacgca cctcaaattg acaaagaagt gaccatttgt cagaataatg 3121 aggattttgc tgttctgaaa cctgtagatc cagatggacc tgaaaatgga ccaccttttc 3181 aattctttct ggataattct gccagtaaaa actggaacat agaagaaaag gatggtaaaa 3241 ctgccattct tcgtcaacgg caaaatcttg attataacta ttattctgtg cctattcaaa 3301 taaaagacag gcatggttta gttgcaacac atatgttaac agtgagagta tgtgactgtt 3361 caactccatc tgagtgtaga atgaaggata aaagtacaag agacgttaga ccaaatgtaa 3421 tacttggaag atgggctatt cttgctatgg tgttgggttc tgtattgtta ttatgtattc 3481 tgtttacatg tttctgtgtc actgctaaga gaacagtcaa gaaatgtttt ccagaagaca 3541 tagcccagca aaatttaatt gtatcaaata ctgaaggacc tggagaagaa gtaacggaag 3601 caaatattag actccccatg cagacatcca acatttgtga cacaagcatg tctgttggta 3661 ctgttggtgg ccagggaatc aaaacacagc aaagttttga gatggtcaaa ggaggctaca 3721 ctttggattc caacaaagga ggtggacatc agaccttgga gtccgtcaag ggagtggggc 3781 agggagatac tggcagatat gcgtacacgg actggcagag tttcacccaa cctcggcttg 3841 gcgaaaaggt gtatttgtgt ggacaagatg aggagcataa acattgtgaa gactacgttt 3901 gttcgtataa ctatgaaggc aaaggttctc tggccggctc agtaggttgc tgcagcgatc 3961 ggcaggaaga agagggactg gagtttctag atcacctgga acccaaattt aggacattag 4021 caaagacatg catcaagaaa taaggatcca ccggatctag ataactgatc ataatcagcc 4081 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 4141 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 4201 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 4261 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt 4321 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 4381 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 4441 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 4501 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 4561 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 4621 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 4681 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 4741 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 4801 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 4861 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt 4921 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 4981 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 5041 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 5101 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5161 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 5221 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 5281 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 5341 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 5401 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 5461 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 5521 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 5581 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 5641 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 5701 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 5761 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 5821 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 5881 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 5941 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 6001 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 6061 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 6121 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 6181 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 6241 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 6301 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 6361 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 6421 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 6481 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 6541 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 6601 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 6661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 6721 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 6781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 6841 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 6901 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 6961 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 7021 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 7081 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 7141 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 7201 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 7261 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 7321 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca 7381 tgcattagtt attaatag //