LOCUS Exported 6932 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV-AML1-ETO SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6932) AUTHORS Meyers S, Lenny N, Hiebert SW TITLE The t(8;21) fusion protein interferes with AML-1B-dependent transcriptional activation. JOURNAL Mol Cell Biol. 1995 Apr . 15(4):1974-82. PUBMED 7891692 REFERENCE 2 (bases 1 to 6932) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6932 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 178..557 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 558..761 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 712..732 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 833..849 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 833..849 /label=pBluescriptSK /note="For pBluescript vector" polyA_signal 3133..3755 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3332..3351) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(3779..3799) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3784..4113 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3964..4099 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4026..4045 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(4151..4170) /label=T7 /note="T7 promoter, forward primer" promoter complement(4152..4170) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4184..4200) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4184..4200) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4197..4219) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4208..4224 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4232..4262) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4277..4298 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4415..4432) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4586..5174) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4666..4685) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5345..6205) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5968..5987 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6206..6310) /gene="bla" /label=AmpR promoter rep_origin 6337..6792 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6424..6443) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6634..6655 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6918..8 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6932..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca 61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt 121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac 181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag 541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt 601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg 721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 781 attcagatct ggtaccacgc gtatcgataa gcttgcatgc ctgcaggtcg actctagaac 841 tagtggatcc gttgtgatgc gtatccccgt agatgccagc acgagccgcc gcttcacgcc 901 gccttccacc gcgctgagcc caggcaagat gagcgaggcg ttgccgctgg gcgccccgga 961 cgccggcgct gccctggccg gcaagctgag gagcggcgac cgcagcatgg tggaggtgct 1021 ggccgaccac ccgggcgagc tggtgcgcac cgacagcccc aacttcctct gctccgtgct 1081 gcctacgcac tggcgctgca acaagaccct gcccatcgct ttcaaggtgg tggccctagg 1141 ggatgttcca gatggcactc tggtcactgt gatggctggc aatgatgaaa actactcggc 1201 tgagctgaga aatgctaccg cagccatgaa gaaccaggtt gcaagattta atgacctcag 1261 gtttgtcggt cgaagtggaa gagggaaaag cttcactctg accatcactg tcttcacaaa 1321 cccaccgcaa gtcgccacct accacagagc catcaaaatc acagtggatg ggccccgaga 1381 acctcgaaat cgtactgaga agcactccac aatgccagac tcacctgtgg atgtgaagac 1441 gcaatctagg ctgactcctc caacaatgcc acctccccca actactcaag gagctccaag 1501 aaccagttca tttacaccga caacgttaac taatggcacg agccattctc ctacagcctt 1561 gaatggcgcc ccctcaccac ccaatggctt cagcaatggg ccttcctctt cttcctcctc 1621 ctctctggct aatcaacagc tgcccccagc ctgtggtgcc aggcaactca gcaagctgaa 1681 aaggttcctt actaccctgc agcagtttgg caatgacatt tcacccgaga taggagaaag 1741 agttcgcacc ctcgttctgg gactagtgaa ctccactttg acaattgaag aatttcattc 1801 caaactgcaa gaagctacta acttcccact gagacctttt gtcatcccat ttttgaaggc 1861 caacttgccc ctgctgcagc gtgagctcct ccactgcgca agactggcca aacagaaccc 1921 tgcccagtac ctcgcccagc atgaacagct gcttctggat gccagcacca cctcacctgt 1981 tgactcctca gagctgcttc tcgatgtgaa cgaaaacggg aagaggcgaa ctccagacag 2041 aaccaaagaa aatggctttg acagagagcc tttgcactca gaacatccaa gcaagcgacc 2101 atgcactatt agcccaggcc agcggtacag tccaaataac ggcttatcct accagcccaa 2161 tggcctgcct caccctaccc cacctccacc tcagcattac cgtttggatg atatggccat 2221 tgcccaccac tacagggact cctatcgaca ccccagccac agggacctca gggacagaaa 2281 cagacctatg gggttgcatg gcacacgtca agaagaaatg attgatcaca gactaacaga 2341 cagagaatgg gcagaagagt ggaaacatct tgaccatctg ttaaactgca taatggacat 2401 ggtagaaaaa acaaggcgat ctctcaccgt actaaggcgg tgtcaagaag cagaccggga 2461 agaattgaat tactggatcc ggcggtacag tgacgccgag gacttaaaaa aaggtggcgg 2521 cagtagcagc agccactcta ggcagcagag tcccgtcaac ccagacccag ttgcactaga 2581 cgcgcatcgg gaattccttc acaggcctgc gtctggatac gtgccagagg agatctggaa 2641 gaaagctgag gaggccgtca atgaggtgaa gcgccaggcg atgacggagc tgcagaaggc 2701 cgtgtctgag gcggagcgga aagcccacga catgatcaca acagagaggg ccaagatgga 2761 gcgcacggtc gccgaggcca aacggcaggc ggcggaggac gcactggcag ttatcaatca 2821 gcaggaggat tcaagcgaga gttgctggaa ttgtggccgt aaagcgagtg aaacctgcag 2881 tggctgtaac acagcccgat actgtggctc attttgccag cacaaagact gggagaagca 2941 ccatcacatc tgtggacaga ccctgcaggc ccagcagcag ggagacacac ctgcagtcag 3001 ctcctctgtc acgcccaaca gcggggctgg gagcccgatg gacacaccac cagcagccac 3061 tccgaggtca accaccccgg gaaccccttc caccatagag acaacccctc gctctcgact 3121 ctagaggatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa 3181 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct 3241 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa 3301 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg 3361 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag 3421 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt 3481 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg 3541 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc 3601 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg 3661 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca 3721 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg 3781 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3841 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 3901 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3961 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 4021 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 4081 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag 4141 aaagttaatt ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg 4201 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 4261 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 4321 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 4381 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 4441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 4501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 4561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 4621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 4681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 4741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 4801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 4861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 4921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 4981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 5041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 5281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 5341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 5401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 5461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 5521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 5581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 5641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 5701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 5761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 5821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 5881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 5941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 6241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 6301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa 6361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 6421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 6481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 6541 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 6601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 6661 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 6721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 6781 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 6841 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 6901 ggtaacgcca gggttttccc agtcacgacg tt //