LOCUS Exported 5653 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMVTNT-T7-KPNA2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5653) AUTHORS Kelley JB, Talley AM, Spencer A, Gioeli D, Paschal BM TITLE Karyopherin alpha7 (KPNA7), a divergent member of the importin alpha family of nuclear import receptors. JOURNAL BMC Cell Biol. 2010 . 11():63. PUBMED 20701745 REFERENCE 2 (bases 1 to 5653) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5653 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 143..522 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 523..726 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 677..697 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 723..747 /label=LNCX /note="Human CMV promoter, forward primer" intron 862..994 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 977..996 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 1039..1057 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 1063..1081 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1063..1080 /label=SP6 /note="SP6 promoter, forward primer" regulatory 1112..1121 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 2772..2893 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2783..2802) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2837..2856 /label=SV40pA-R /note="SV40 polyA, reverse primer" rep_origin 3074..3529 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3161..3180) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3371..3392 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3596..3615) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3715..3737 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3775..3793) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3861..3965 /gene="bla" /label=AmpR promoter CDS 3966..4826 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4184..4203) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4997..5585 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5486..5505 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gatcttcaat attggccatt agccatatta ttcattggtt atatagcata aatcaatatt 61 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca 121 tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata gtaatcaatt 181 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 241 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 301 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 361 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc 421 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct 481 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 541 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 601 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat 661 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 721 agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt ttatcacagt 781 taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag ctgcagaagt 841 tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta aggagaccaa 901 tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt 961 cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt caattacagc 1021 tcttaaggct agagtactta atacgactca ctataggcta gcatttaggt gacactatag 1081 aatacaagct acttgttctt tttgcactcg agccaccatg gccagcatga ccggcggcca 1141 gcagatgggc gaattctcca ccaacgagaa tgctaataca ccagctgccc gtcttcacag 1201 attcaagaac aagggaaaag acagtacaga aatgaggcgt cgcagaatag aggtcaatgt 1261 ggagctgagg aaagctaaga aggatgacca gatgctgaag aggagaaatg taagctcatt 1321 tcctgatgat gctacttctc cgctgcagga aaaccgcaac aaccagggca ctgtaaattg 1381 gtctgttgat gacattgtca aaggcataaa tagcagcaat gtggaaaatc agctccaagc 1441 tactcaagct gccaggaaac tactttccag agaaaaacag ccccccatag acaacataat 1501 ccgggctggt ttgattccga aatttgtgtc cttcttgggc agaactgatt gtagtcccat 1561 tcagtttgaa tctgcttggg cactcactaa cattgcttct gggacatcag aacaaaccaa 1621 ggctgtggta gatggaggtg ccatcccagc attcatttct ctgttggcat ctccccatgc 1681 tcacatcagt gaacaagctg tctgggctct aggaaacatt gcaggtgatg gctcagtgtt 1741 ccgagacttg gttattaagt acggtgcagt tgacccactg ttggctctcc ttgcagttcc 1801 tgatatgtca tctttagcat gtggctactt acgtaatctt acctggacac tttctaatct 1861 ttgccgcaac aagaatcctg cacccccgat agatgctgtt gagcagattc ttcctacctt 1921 agttcggctc ctgcatcatg atgatccaga agtattagca gatacctgct gggctatttc 1981 ctaccttact gatggtccaa atgaacgaat tggcatggtg gtgaaaacag gagttgtgcc 2041 ccaacttgtg aagcttctag gagcttctga attgccaatt gtgactcctg ccctaagagc 2101 catagggaat attgtcactg gtacagatga acagactcag gttgtgattg atgcaggagc 2161 actcgccgtc tttcccagcc tgctcaccaa ccccaaaact aacattcaga aggaagctac 2221 gtggacaatg tcaaacatca cagccggccg ccaggaccag atacagcaag ttgtgaatca 2281 tggattagtc ccattccttg tcagtgttct ctctaaggca gattttaaga cacaaaagga 2341 agctgtgtgg gccgtgacca actataccag tggtggaaca gttgaacaga ttgtgtacct 2401 tgttcactgt ggcataatag aaccgttgat gaacctctta actgcaaaag ataccaagat 2461 tattctggtt atcctggatg ccatttcaaa tatctttcag gctgctgaga aactaggtga 2521 aactgagaaa cttagtataa tgattgaaga atgtggaggc ttagacaaaa ttgaagctct 2581 acaaaaccat gaaaatgagt ctgtgtataa ggcttcgtta agcttaattg agaagtattt 2641 ctctgtagag gaagaggaag atcaaaacgt tgtaccagaa actacctctg aaggctacac 2701 tttccaagtt caggatgggg ctcctgggac ctttaacttt tagcccgggc ggccgcttcg 2761 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 2821 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 2881 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat 2941 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc 3001 gggctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 3061 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 3121 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 3181 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 3241 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 3301 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 3361 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 3421 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 3481 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt 3541 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 3601 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 3661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 3721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 3781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 3841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 3901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 3961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 4021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 4081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 4141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 4201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 4261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 4321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 4381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 4441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 4501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 4561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 4621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 4681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 4741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 4801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 4861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 4921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 4981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 5041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 5101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 5161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 5221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 5281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 5341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 5401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 5461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 5521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 5581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 5641 acatggctcg aca //