LOCUS Exported 7146 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Tomato expressing plasmid. ACCESSION . VERSION . KEYWORDS MSCV-IRES_Tomato SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7146) AUTHORS Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA, Armstrong SA, Kentsis A TITLE MEF2C Phosphorylation Is Required for Chemotherapy Resistance in Acute Myeloid Leukemia. JOURNAL Cancer Discov. 2018 Apr;8(4):478-497. doi: 10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5. PUBMED 29431698 REFERENCE 2 (bases 1 to 7146) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7146 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 149..665 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" misc_feature 729..1070 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" CDS 1137..1553 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1483..1505 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1521..1537 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1630..2182 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(1774..1791) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2001..2020 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 2194..3624 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" LTR 3689..4203 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" protein_bind 4365..4381 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4389..4419) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4434..4455 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4572..4589) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4743..5331) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4823..4842) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5502..6362) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6125..6144 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6363..6467) /gene="bla" /label=AmpR promoter primer_bind 6535..6553 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(6591..6613) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 6713..6732 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6926..6948 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 7076..7095 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" ORIGIN 1 cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt 61 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga 121 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc 181 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt 241 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta 301 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct 361 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc 421 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct 481 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac 541 tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat ccgaatcgtg 601 gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct cgggggtctt 661 tcatttggag gttccaccga gatttggaga cccctgccca gggaccaccg acccccccgc 721 cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg 781 ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc tgtatctggc 841 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca 901 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga 961 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt 1021 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca 1081 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc 1141 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg 1201 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat 1261 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc 1321 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca 1381 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc 1441 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt 1501 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggaatta 1561 gatctctcga ggaggaggat ccttgggggc ggatcccccg ggctgcagga attccgcccc 1621 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 1681 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 1741 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 1801 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 1861 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 1921 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 1981 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 2041 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat 2101 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 2161 tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga ggaggtcatc 2221 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag 2281 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 2341 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc 2401 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc 2461 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc 2521 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac 2581 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag 2641 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag 2701 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa 2761 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac 2821 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc 2881 accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc 2941 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag 3001 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 3061 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg 3121 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc 3181 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc 3241 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc 3301 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 3361 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag 3421 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc 3481 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag 3541 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac 3601 ggcatggacg agctgtacaa gtaagaattc gatatcaagc ttatcgataa aataaaagat 3661 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct 3721 agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc 3781 agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa 3841 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc 3901 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct 3961 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc 4021 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact 4081 gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt 4141 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt 4201 catgggtaac agtttcttga agttggagaa caacattctg agggtaggag tcgaatatta 4261 agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac tcctgaaata 4321 gttcattatg gacagcgcag aaagagctgg ggagaattgt gaaattgtta tccgctcaca 4381 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 4441 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 4501 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 4561 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 4621 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 4681 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 4741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 4801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 4861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 4921 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 4981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 5161 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 5221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 5281 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 5341 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 5401 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 5461 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 5521 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 5581 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 5641 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 5701 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 5761 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 5821 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 5881 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 5941 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 6001 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 6061 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 6121 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 6181 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 6241 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 6301 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 6361 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 6421 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 6481 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 6541 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 6601 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 6661 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca 6721 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 6781 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 6841 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 6901 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga 6961 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac 7021 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga 7081 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc 7141 gtccgg //