LOCUS Exported 11653 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION rfp ""test"" strain. ACCESSION . VERSION . KEYWORDS pJMP1161 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11653) AUTHORS Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS TITLE Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi. JOURNAL Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 10.1038/s41564-018-0327-z. PUBMED 30617347 REFERENCE 2 (bases 1 to 11653) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11653 /organism="synthetic DNA construct" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1697..1716 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1935..2039) /gene="bla" /label=AmpR promoter primer_bind 2107..2125 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(2163..2185) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 2285..2304 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2718..2734 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 2719..2735 /label=KS primer /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(3192..3725) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /label=GmR /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3914..3942) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" protein_bind 4059..4106 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(4244..4921) /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" RBS 4928..4939 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS complement(5388..6470) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 6432..6451 /label=LacI-R /note="LacI, reverse primer" promoter complement(6471..6548) /gene="lacI (mutant)" /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." protein_bind 6900..6914 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 6921..6937 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 6979..11082 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11092..11181 /codon_start=1 /product="3 tandem Myc epitope tags" /label=3xMyc /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" mobile_element 11337..11502 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga cgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcaggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gatctcggct 3181 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt 3241 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag 3301 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca 3361 atgccctgcc tccggtgctc tccggagact gcgagatcat agatatagat ctcactacgc 3421 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg 3481 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc 3541 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca 3601 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt 3661 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt 3721 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt 3781 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac 3841 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg 3901 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat 3961 ccgtttccac ggtgtgcgtc catgggcaaa tattatacgc aaggcgacaa ggctcgagag 4021 ttcaacagac agccttcgtt cttatggccg ttcaggagga agttcctatt ccgaagttcc 4081 tattctctag aaagtatagg aacttcttga gaagagaaaa gaaaaccgcc gatcctgtcc 4141 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaacga 4201 atgacgaaaa ggctgtctga gcaaatccag gaggtcgttt ttattaagca ccggtggagt 4261 gacgaccttc agcacgttcg tactgttcaa cgatggtgta gtcttcgttg tgggaggtga 4321 tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg aaccggtttt ttagccatgt 4381 aggtggtttt aacttcagcg tcgtagtgac caccgtcttt cagtttcaga cgcattttga 4441 tttcaccttt cagagcaccg tcttccgggt acatacgttc ggtggaagct tcccaaccca 4501 tggttttttt ctgcataacc ggaccgtcgg acgggaagtt ggtaccacgc agtttaactt 4561 tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt aacggtaaca acaccaccgt 4621 cttcgaagtt cataacacgt tcccatttga aaccttccgg gaaggacagt ttcaggtagt 4681 ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc gtactggaac tgcggggaca 4741 ggatgtccca agcgaacggc agcggaccac ctttggtaac tttcagttta gcggtctggg 4801 taccttcgta cggacgacct tcaccttcac cttcgatttc gaactcgtga ccgttaacgg 4861 aaccttccat acgaactttg aaacgcatga actctttgat aacgtcttcg ctactcgcca 4921 tggtaccttt ctcctcttta attaattcag atctattata cctaggactg agctagctgt 4981 caaattcacc accctgaatt gactctcaaa ccgtattagc ccggtatttt ggaaatagcg 5041 gaagcactga cttttgttat caataaaaaa ggccccccgt tagggaggcc ttattgttcg 5101 tctactcgga agagcgagag acaacagaac ggtcagccac atgaattctt tcagctcagt 5161 cgataggtag taggcaagag tagtcgcacc tttggtcgaa aaaaaaagcc cgcactgtca 5221 ggtgcgggct tttttctgtg tttccccaaa agtaaaaacc cgccgaagcg ggtttttacg 5281 taaaacaggt gaaactgacc agacgagaag gctttggagg tgataatggg gctcaaggac 5341 cctggggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 5401 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5461 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 5521 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 5581 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 5641 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 5701 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 5761 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 5821 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 5881 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 5941 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 6001 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 6061 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 6121 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 6181 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 6241 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 6301 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 6361 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 6421 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 6481 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcaccattcg 6541 atggtgtcaa cgtaaatgca tgccgcttgg gaggcagata ggtaggcatg gcccccattt 6601 tcaatacaag caacgcatga gaaagccccc ggaagatcac cttccggggg cttttttatt 6661 gcgctccggc aattaaaaaa gcggctaacc acgccgcttt ttttacgtct gcaggcgaat 6721 tgatctggtt tgacagctta tcaccaagcc agttacctcg gttcaaagag ttggtagctc 6781 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta 6841 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tattataaat 6901 gtgagcggat aacattgaca ttgtgagcgg ataacaagat actgagcaca tcagcaggtt 6961 tcacacagga aaactagtat ggataagaaa tactcaatag gcttagctat cggcacaaat 7021 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt 7081 ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac 7141 agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt 7201 cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat 7261 gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa 7321 cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact 7381 atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc 7441 tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga gggagattta 7501 aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac ctacaatcaa 7561 ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat tctttctgca 7621 cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg tgagaagaaa 7681 aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa ttttaaatca 7741 aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta cgatgatgat 7801 ttagataatt tattggcgca aattggagat caatatgctg atttgttttt ggcagctaag 7861 aatttatcag atgctatttt actttcagat atcctaagag taaatactga aataactaag 7921 gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga cttgactctt 7981 ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt ttttgatcaa 8041 tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga attttataaa 8101 tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt gaaactaaat 8161 cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc ccatcaaatt 8221 cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc atttttaaaa 8281 gacaatcgtg agaagattga aaaaatcttg acttttcgaa tcccttatta tgttggtcca 8341 ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga aacaattacc 8401 ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc 8461 atgacaaact ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca tagtttgctt 8521 tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac tgaaggaatg 8581 cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt actcttcaaa 8641 acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa aatagaatgt 8701 tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt aggtacctac 8761 catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga aaatgaagat 8821 atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat gattgaggaa 8881 agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct taaacgtcgc 8941 cgttatactg gttggggacg tttgtctcga aaattgatta atggtattag ggataagcaa 9001 tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg caattttatg 9061 cagctgatcc atgatgatag tttgacattt aaagaagaca ttcaaaaagc acaagtgtct 9121 ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc tgctattaaa 9181 aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat 9241 aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca aaagggccag 9301 aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag 9361 attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct ctatctctat 9421 tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa tcgtttaagt 9481 gattatgatg tcgatgccat tgttccacaa agtttcctta aagacgattc aatagacaat 9541 aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa 9601 gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt aatcactcaa 9661 cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact tgataaagct 9721 ggttttatca aacgccaatt ggttgaaact cgccaaatca ctaagcatgt ggcacaaatt 9781 ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg agaggttaaa 9841 gtgattacct taaaatctaa attagtttct gacttccgaa aagatttcca attctataaa 9901 gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga 9961 actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg tgattataaa 10021 gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa agcaaccgca 10081 aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac acttgcaaat 10141 ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga aattgtctgg 10201 gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca agtcaatatt 10261 gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg agtcaatttt accaaaaaga 10321 aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt 10381 gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa agggaaatcg 10441 aagaagttaa aatccgttaa agagttacta gggatcacaa ttatggaaag aagttccttt 10501 gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa aaaagactta 10561 atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa acggatgctg 10621 gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa atatgtgaat 10681 tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga taacgaacaa 10741 aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga gcaaatcagt 10801 gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct tagtgcatat 10861 aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca tttatttacg 10921 ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat tgatcgtaaa 10981 cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc catcactggt 11041 ctttatgaaa cacgcattga tttgagtcag ctaggaggtg acgcggccgc ggagcagaaa 11101 ctcatctctg aagaagatct ggaacaaaag ttgatttcag aagaagatct ggaacagaag 11161 ctcatctctg aggaagatct gtaataaggc gcgcctcctt aatgggactt gcagcctcgg 11221 taccaaattc cagaaaagac acccgaaagg gtgttttttc gttttggtcc cacagaatga 11281 gcatcatggc tctagtcgac atcggagaag agtacggctc ttttaaccgc ctcaagaacc 11341 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg 11401 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc 11461 atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc 11521 tgttatgttt gggcgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 11581 attgggcgct cttccgcttc ctcgctcact gacccgctgc gctcggtcgt tcggctgcgg 11641 cgagcggtat cag //