LOCUS       Exported                 608 bp ds-DNA     linear   SYN 28-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA5-TO-PV-NLS-Dsred
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Rodrigues MA, Gomes DA, Leite MF, Grant W, Zhang L, Lam W, Cheng YC,
            Bennett AM, Nathanson MH
  TITLE     Nucleoplasmic calcium is required for cell proliferation.
  JOURNAL   J Biol Chem. 2007 Jun 8. 282(23):17061-8.
  PUBMED    17420246
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     50..74
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag
       61 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      121 gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc gggccccccc
      181 tcgaggtcga catgtcgatg acagacttgc tcagcgctga ggacatcaag aaggcgatag
      241 gagcctttac tgctgcagac tccttcgacc acaaaaagtt cttccagatg gtgggcctga
      301 agaaaaagag tgcggatgat gtgaagaagg tgttccacat tctggacaaa gacaaaagtg
      361 gcttcattga ggaggatgag ctggggtcca ttctgaaggg cttctcctca gatgccagag
      421 acttgtctgc taaggaaaca aagacgctga tggctgctgg agacaaggac ggggacggca
      481 agattggggt tgaagaattc tccactctgg tggccgaaag cgcggccgca atggcctcct
      541 ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg catggagggc accgtgaacg
      601 gccacgag
//
