LOCUS       Exported                7435 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plasmid for expression of gene of interest in Lactobacillus 
            plantarum and L. sakei.
ACCESSION   .
VERSION     .
KEYWORDS    pSIP403
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Sorvig E, Mathiesen G, Naterstad K, Eijsink VG, Axelsson L
  TITLE     High-level, inducible gene expression in Lactobacillus sakei and 
            Lactobacillus plantarum using versatile expression vectors.
  JOURNAL   Microbiology. 2005 Jul;151(Pt 7):2439-49. doi: 
            10.1099/mic.0.28084-0.
  PUBMED    16000734
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             156..1964
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQGGKQ"
     rep_origin      2312..2899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2800..2819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3053..3070
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accggtgggc ccatattaac gtttaaccga taaagttgaa cgttaatatt ttttttgcgc
       61 agaaatggta aattgaagca taatagtctt gtaaggtatt tagctggctg gcgtaaagta
      121 tgctttataa aataatatat aggagtatga ttcccatggt acgtcctgta gaaaccccaa
      181 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
      241 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
      301 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
      361 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
      421 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
      481 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
      541 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
      601 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
      661 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
      721 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
      781 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
      841 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
      901 tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg
      961 tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct
     1021 actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg
     1081 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     1141 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     1201 ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca
     1261 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     1321 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     1381 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     1441 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     1501 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     1561 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     1621 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     1681 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     1741 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     1801 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     1861 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     1921 ctggcatgaa cttcggtgaa aaaccgcagg gaggcaaaca atgatctaga ctcgaggaat
     1981 tcggtacccc gggttcgaag gcgccaagct tcaaattaca gcacgtgttg ctttgattga
     2041 tagccaaaaa gcagcagttg ataaagcaat tactgatatt gctgaaaaat tgtaatttat
     2101 aaataaaaat caccttttag aggtggtttt tttatttata aattattcgt ttgatttcgc
     2161 tttcgataga acaatcaaag cgagaataag gaagataaat cccataaggg cgggagcaga
     2221 atgtccgaga ctaattcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2281 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2341 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2401 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2461 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2521 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2581 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2641 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2701 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2761 gcagggtcgg aacaggaggc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2881 ggggcggagc ctatcgaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3061 agtgagcgag aaggattatt cggctggttg agacgttaaa atgataaagg ttgtattaat
     3121 cttatattac ggttataatg tactcaactt aataaatgaa cgcaaaaaaa agaaccctca
     3181 acttagcaga gttaggattc acgacttatc agcacaacct gataagattt tcgatagcaa
     3241 gtactaccaa tacaagctat ctaacttggt actattataa catgtaggct aagtttttca
     3301 accattgata cttaaagtaa acggttgtta tcgggaatct taacagaaac ctgatagcaa
     3361 ccgttttttt gttattcaat ggttagcaac catcaaagca actaaaggct ggaaacctgt
     3421 tcttagctag taaaacctcc cgtgagtgtc gttcgtgacc ccgcttgcag ttaacaacat
     3481 aggtatgcta aaccttgtcg agatcaacgc gactaaagac gtggctggaa gactaggaaa
     3541 tgatacggac aggctaacta ttaacgcaga ttattcgggt tgctgctaaa accaactcta
     3601 ataatagtta gtgcaagggc tggttgagct taaattgtct gataaagagt tctctcttta
     3661 tactgcaaaa gaagcgcagt tattcacgat taggataact gtttgagaga gcctaagggc
     3721 ttgacccttg atggtttaag caccgctatg cgtgcgggat ccccttagaa gcaaacttaa
     3781 gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa gttaaattag
     3841 atgctaaaaa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
     3901 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
     3961 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
     4021 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttcagga
     4081 gagattacat gaacaaaaat ataaatatct caaacttttt aacgagtgaa aaagtactca
     4141 accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa attggaacag
     4201 gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg tctattgaat
     4261 tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact cgtgtcactt
     4321 taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat aaaattgttg
     4381 ggaatattcc ttacaattta agcacacaaa ttattaaaaa agtggttttt gaaagccgtg
     4441 cgtctgacat ctatctgact gttgaagaag gattctacaa gcgtaccttg gatattcacc
     4501 gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt aagctgccag
     4561 cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt acccgccata
     4621 ccacagatgt tccagataaa tattggaagc tatataagta ctttgtttca aaatgggtca
     4681 atcgagaata tcgtcaactg tttactaaaa atcagtttcg tcaagcaatg aaacacgcca
     4741 aagtaaacaa tttaagtacc attacttatg agcaagtatt gtctattttt aatagttatc
     4801 tattatttaa cgggaggaaa taattctatg agtcgctttt ttaaatttgg aaagttacac
     4861 gttactaaag ggaatggaga ccggggtcga cccttcaata gagttcttaa cgttaatccg
     4921 aaaaaaacta acgttaatat taaaaaataa gatccgcttg tgaattatgt ataatttgat
     4981 tagactaaag aataggagaa agtatgatga tatttaaaaa actttctcgt taagataggt
     5041 tgttggtgag catgttatat acggatgtat cggtttcctt aatgcaaaat tttgttgcta
     5101 tcttattaat ttttctatta tatagatata ttcaaagaaa gataacattt aaacggatca
     5161 tattagatat tttaatagcg attatttttt caatattata tctgtttatt tcagatgcgt
     5221 cattacttgt aatggtatta atgcgattag ggtggcattt tcatcaacaa aaagaaaata
     5281 agataaaaac gactgataca gctaatttaa ttctaattat cgtgatccag ttattgttag
     5341 ttgcggttgg gactattatt agtcagttta ccatatcgat tatcaaaagt gatttcagcc
     5401 aaaatatatt gaacaatagt gcaacagata taactttatt aggtattttc tttgctgttt
     5461 tatttgacgg cttgttcttt atattattga agaataagcg gactgaatta caacatttaa
     5521 atcaagaaat cattgaattt tcgttagaaa aacaatattt tatatttata tttattttat
     5581 ttatagtaat agaaattatt ttagcagttg ggaatcttca aggagtaaca gccacgatat
     5641 tattaaccat tatcattatt ttttgtgtcc ttatcgggat gactttttgg caagtgatgc
     5701 tttttttgaa ggcttattcg attcgccaag aagccaatga ccaattggtc cggaatcaac
     5761 aacttcaaga ttatctagtc aatatcgaac agcagtacac cgaattacgg cgatttaagc
     5821 atgattatca aaacatctta ttatcgttgg agagttttgc cgaaaagggc gatcagcaac
     5881 agtttaaggc gtattaccaa gaattattag cacaacggcc aattcaaagt gaaatccaag
     5941 gggcagtcat tgcacaactc gactacttga aaaatgatcc tattcgagga ttagtcattc
     6001 aaaagttttt ggcagccaaa caggctggtg ttactttaaa attcgaaatg accgaaccaa
     6061 tcgaattagc aaccgctaat ctattaacgg ttattcggat tatcggtatt ttattagaca
     6121 atgcgattga acaagccgtt caagaaaccg atcaattggt gagttgtgct ttcttacaat
     6181 ctgatggttt aatcgaaatt acgattgaaa atacggccag tcaagttaag aatctccaag
     6241 cattttcaga gttaggctat tcaacgaaag gcgctggtcg ggggactggt ttagctaatg
     6301 tgcaggattt gattgccaaa caaaccaatt tattcttaga aacacagatt gaaaatagaa
     6361 agttacgaca gacattgatg attacggagg aaacttaatt tgtatcccgt ttatttatta
     6421 gaggatgatt tacagcaaca agcgatttat cagcaaatta tcgcgaatac gattatgatt
     6481 aacgaatttg caatgacttt aacatgcgct gccagtgata ctgagacatt gttggcggca
     6541 attaaggatc agcaacgagg tttattcttt ttggatatgg aaattgagga taaccgccaa
     6601 gccggtttag aagtggcaac taagattcgg cagatgatgc cgtttgcgca aattgtcttc
     6661 attacaaccc acgaggaact gacattatta acgttagaac gaaaaatagc gcctttagat
     6721 tacattctca aggaccaaac aatggctgaa atcaaaaggc aattgattga tgatctattg
     6781 ttagctgaga agcaaaacga ggcggcagcg tatcaccgag aaaatttatt tagttataaa
     6841 ataggtcctc gctttttctc attaccatta aaggaagttg tttatttata tactgaaaaa
     6901 gaaaatccgg gtcatattaa tttgttagcc gttaccagaa aggttacttt tccaggaaat
     6961 ttaaatgcgc tggaagccca atatccaatg ctctttcggt gtgataaaag ttacttagtt
     7021 aacctatcta atattgccaa ttatgacagt aaaacacgga gtttaaaatt tgtagatggc
     7081 agtgaggcaa aagtctcgtt ccggaaatca cgggaactag tggccaaatt aaaacaaatg
     7141 atgtagcgcc tgcaggcacg ccaaatgatc ccagtaaaaa gccacccgca tggcgggtgg
     7201 ctttttatta gccctagaag ggcttcccac acgcatttca gcgccttagt gccttagttt
     7261 gtgaatcata ggtggtatag tcccgaaata cccgtctaag gaattgtcag ataggcctaa
     7321 tgactggctt ttataatatg agataatgcc gactgtactt tttacagtcg gttttctaat
     7381 gtcactaacc tgccccgtta gttgaagaag gtttttatat tacagctcca gatct
//
