LOCUS Exported 11801 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION rfp ""test"" strain. ACCESSION . VERSION . KEYWORDS pJMP1185 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11801) AUTHORS Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS TITLE Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi. JOURNAL Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 10.1038/s41564-018-0327-z. PUBMED 30617347 REFERENCE 2 (bases 1 to 11801) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11801 /organism="synthetic DNA construct" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1697..1716 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1935..2039) /gene="bla" /label=AmpR promoter primer_bind 2107..2125 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(2163..2185) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 2285..2304 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2718..2734 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 2719..2735 /label=KS primer /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(3196..3990) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3307..3326) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 3917..3936 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter complement(4101..4129) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" protein_bind 4207..4254 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(4392..5069) /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" RBS 5076..5087 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS complement(5536..6618) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 6580..6599 /label=LacI-R /note="LacI, reverse primer" promoter complement(6619..6696) /gene="lacI (mutant)" /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." protein_bind 7048..7062 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 7069..7085 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 7127..11230 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11240..11329 /codon_start=1 /product="3 tandem Myc epitope tags" /label=3xMyc /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" mobile_element 11485..11650 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga cgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcaggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gtcatttcga 3181 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga 3241 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc 3301 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg 3361 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc 3421 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa 3481 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc 3541 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca 3601 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc 3661 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca 3721 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc 3781 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt 3841 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca 3901 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga 3961 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc 4021 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agttttgagc 4081 gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg 4141 ccttcatccg tttccacggt ctcgagagtt caacagacag ccttcgttct tatggccgtt 4201 caggaggaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcttgaga 4261 agagaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg 4321 gcggtcttaa gttttttggc tgaaacgaat gacgaaaagg ctgtctgagc aaatccagga 4381 ggtcgttttt attaagcacc ggtggagtga cgaccttcag cacgttcgta ctgttcaacg 4441 atggtgtagt cttcgttgtg ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc 4501 ggcagctgaa ccggtttttt agccatgtag gtggttttaa cttcagcgtc gtagtgacca 4561 ccgtctttca gtttcagacg cattttgatt tcacctttca gagcaccgtc ttccgggtac 4621 atacgttcgg tggaagcttc ccaacccatg gtttttttct gcataaccgg accgtcggac 4681 gggaagttgg taccacgcag tttaactttg tagatgaact caccgtcttg cagggaggag 4741 tcctgggtaa cggtaacaac accaccgtct tcgaagttca taacacgttc ccatttgaaa 4801 ccttccggga aggacagttt caggtagtcc gggatgtcag ccgggtgttt aacgtaagct 4861 ttggaaccgt actggaactg cggggacagg atgtcccaag cgaacggcag cggaccacct 4921 ttggtaactt tcagtttagc ggtctgggta ccttcgtacg gacgaccttc accttcacct 4981 tcgatttcga actcgtgacc gttaacggaa ccttccatac gaactttgaa acgcatgaac 5041 tctttgataa cgtcttcgct actcgccatg gtacctttct cctctttaat taattcagat 5101 ctattatacc taggactgag ctagctgtca aattcaccac cctgaattga ctctcaaacc 5161 gtattagccc ggtattttgg aaatagcgga agcactgact tttgttatca ataaaaaagg 5221 ccccccgtta gggaggcctt attgttcgtc tactcggaag agcgagagac aacagaacgg 5281 tcagccacat gaattctttc agctcagtcg ataggtagta ggcaagagta gtcgcacctt 5341 tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tccccaaaag 5401 taaaaacccg ccgaagcggg tttttacgta aaacaggtga aactgaccag acgagaaggc 5461 tttggaggtg ataatggggc tcaaggaccc tggggtgcct aatgagtgag ctaacttaca 5521 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5581 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt 5641 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag 5701 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt 5761 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc 5821 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc 5881 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg 5941 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg 6001 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc 6061 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt 6121 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa 6181 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg 6241 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca 6301 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc 6361 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc 6421 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta 6481 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc 6541 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta 6601 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc 6661 cataccgcga aaggttttgc accattcgat ggtgtcaacg taaatgcatg ccgcttggga 6721 ggcagatagg taggcatggc ccccattttc aatacaagca acgcatgaga aagcccccgg 6781 aagatcacct tccgggggct tttttattgc gctccggcaa ttaaaaaagc ggctaaccac 6841 gccgcttttt ttacgtctgc aggcgaattg atctggtttg acagcttatc accaagccag 6901 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg 6961 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat 7021 cttattaatc agataaaata ttataaatgt gagcggataa cattgacatt gtgagcggat 7081 aacaagatac tgagcacatc agcaggtttc acacaggaaa actagtatgg ataagaaata 7141 ctcaataggc ttagctatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata 7201 taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa 7261 aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctcaa 7321 acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat 7381 tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt 7441 tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga 7501 agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc 7561 tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg 7621 tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt 7681 tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg 7741 agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct 7801 cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc 7861 attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca 7921 gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca 7981 atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat 8041 cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta 8101 cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga 8161 aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg 8221 gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg 8281 tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt 8341 tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag 8401 acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac 8461 ttttcgaatc ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat 8521 gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg 8581 tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga 8641 aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac 8701 aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa 8761 gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa 8821 agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga 8881 tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga 8941 ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt 9001 atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga 9061 taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa 9121 attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc 9181 agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa 9241 agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc 9301 aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga 9361 tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg 9421 tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga 9481 agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca 9541 attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga 9601 ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatgccattg ttccacaaag 9661 tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg 9721 taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca 9781 acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg 9841 tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg 9901 ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga 9961 aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga 10021 cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca 10081 tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga 10141 atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc 10201 tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt 10261 cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac 10321 taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa 10381 agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt 10441 ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga 10501 ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt 10561 ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg 10621 gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa 10681 aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga 10741 gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga 10801 gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt 10861 gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta 10921 tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc 10981 caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca 11041 agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa 11101 atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc 11161 cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct 11221 aggaggtgac gcggccgcgg agcagaaact catctctgaa gaagatctgg aacaaaagtt 11281 gatttcagaa gaagatctgg aacagaagct catctctgag gaagatctgt aataaggcgc 11341 gcctccttaa tgggacttgc agcctcggta ccaaattcca gaaaagacac ccgaaagggt 11401 gttttttcgt tttggtccca cagaatgagc atcatggctc tagtcgacat cggagaagag 11461 tacggctctt ttaaccgcct caagaaccag ataagtgaaa tctagttcca aactattttg 11521 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac 11581 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg 11641 tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg 11701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 11761 cccgctgcgc tcggtcgttc ggctgcggcg agcggtatca g //