LOCUS Exported 11675 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION rfp ""test"" strain. ACCESSION . VERSION . KEYWORDS pJMP1223 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11675) AUTHORS Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS TITLE Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi. JOURNAL Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 10.1038/s41564-018-0327-z. PUBMED 30617347 REFERENCE 2 (bases 1 to 11675) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11675 /organism="synthetic DNA construct" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1697..1716 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1935..2039) /gene="bla" /label=AmpR promoter primer_bind 2107..2125 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(2163..2185) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 2285..2304 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2718..2734 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 2719..2735 /label=KS primer /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." primer_bind 3808..3827 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(3976..4004) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" protein_bind 4081..4128 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(4266..4943) /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" RBS 4950..4961 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS complement(5410..6492) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind 6454..6473 /label=LacI-R /note="LacI, reverse primer" promoter complement(6493..6570) /gene="lacI (mutant)" /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." protein_bind 6922..6936 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 6943..6959 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 7001..11104 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11114..11203 /codon_start=1 /product="3 tandem Myc epitope tags" /label=3xMyc /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" mobile_element 11359..11524 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga cgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcaggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gttccagtcg 3181 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 3241 cgtattgggc gcatgcataa aaactgttgt aattcattaa gcattctgcc gacatggaag 3301 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 3361 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 3421 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 3481 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 3541 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 3601 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 3661 ttcattgcca tacggaactc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 3721 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 3781 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 3841 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 3901 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tgagcgcata 3961 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc 4021 atccgtttcc acggctcgag agttcaacag acagccttcg ttcttatggc cgttcaggag 4081 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttctt gagaagagaa 4141 aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc 4201 ttaagttttt tggctgaaac gaatgacgaa aaggctgtct gagcaaatcc aggaggtcgt 4261 ttttattaag caccggtgga gtgacgacct tcagcacgtt cgtactgttc aacgatggtg 4321 tagtcttcgt tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc 4381 tgaaccggtt ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct 4441 ttcagtttca gacgcatttt gatttcacct ttcagagcac cgtcttccgg gtacatacgt 4501 tcggtggaag cttcccaacc catggttttt ttctgcataa ccggaccgtc ggacgggaag 4561 ttggtaccac gcagtttaac tttgtagatg aactcaccgt cttgcaggga ggagtcctgg 4621 gtaacggtaa caacaccacc gtcttcgaag ttcataacac gttcccattt gaaaccttcc 4681 gggaaggaca gtttcaggta gtccgggatg tcagccgggt gtttaacgta agctttggaa 4741 ccgtactgga actgcgggga caggatgtcc caagcgaacg gcagcggacc acctttggta 4801 actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt 4861 tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg 4921 ataacgtctt cgctactcgc catggtacct ttctcctctt taattaattc agatctatta 4981 tacctaggac tgagctagct gtcaaattca ccaccctgaa ttgactctca aaccgtatta 5041 gcccggtatt ttggaaatag cggaagcact gacttttgtt atcaataaaa aaggcccccc 5101 gttagggagg ccttattgtt cgtctactcg gaagagcgag agacaacaga acggtcagcc 5161 acatgaattc tttcagctca gtcgataggt agtaggcaag agtagtcgca cctttggtcg 5221 aaaaaaaaag cccgcactgt caggtgcggg cttttttctg tgtttcccca aaagtaaaaa 5281 cccgccgaag cgggttttta cgtaaaacag gtgaaactga ccagacgaga aggctttgga 5341 ggtgataatg gggctcaagg accctggggt gcctaatgag tgagctaact tacattaatt 5401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 5461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt 5521 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag 5581 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg 5641 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc 5701 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc 5761 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc 5821 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag 5881 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa 5941 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc 6001 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc 6061 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt 6121 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc 6181 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga 6241 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc 6301 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag 6361 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt 6421 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt 6481 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc 6541 gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg catgccgctt gggaggcaga 6601 taggtaggca tggcccccat tttcaataca agcaacgcat gagaaagccc ccggaagatc 6661 accttccggg ggctttttta ttgcgctccg gcaattaaaa aagcggctaa ccacgccgct 6721 ttttttacgt ctgcaggcga attgatctgg tttgacagct tatcaccaag ccagttacct 6781 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt 6841 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt 6901 aatcagataa aatattataa atgtgagcgg ataacattga cattgtgagc ggataacaag 6961 atactgagca catcagcagg tttcacacag gaaaactagt atggacaaga agtacagcat 7021 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt 7081 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct 7141 gatcggcgcc ctgctgttcg acagcggaga aacagccgag gccacccggc tgaagagaac 7201 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag 7261 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt 7321 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc 7381 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga 7441 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca 7501 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca 7561 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga 7621 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc 7681 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg 7741 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag 7801 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc 7861 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag 7921 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga 7981 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta 8041 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacatcg atggcggagc 8101 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga 8161 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa 8221 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga 8281 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg 8341 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag 8401 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgccag 8461 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt 8521 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt 8581 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc 8641 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga 8701 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt 8761 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct 8821 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga 8881 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt 8941 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat 9001 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg 9061 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga 9121 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct 9181 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct 9241 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa 9301 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg 9361 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca 9421 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga 9481 actggacatc aaccggctgt ccgactacga tgtggacgct atcgtgcctc agagctttct 9541 gaaggacgac tccatcgata acaaagtgct gactcggagc gacaagaacc ggggcaagag 9601 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc gccagctgct 9661 gaatgccaag ctgattaccc agaggaagtt cgacaatctg accaaggccg agagaggcgg 9721 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat 9781 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaacga 9841 caaactgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg 9901 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc 9961 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga 10021 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca 10081 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa 10141 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg 10201 cgaaacaggc gagatcgtgt gggataaggg ccgggacttt gccaccgtgc ggaaagtgct 10261 gtctatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa 10321 agagtctatc ctgcccaaga ggaacagcga caagctgatc gccagaaaga aggactggga 10381 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc 10441 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac 10501 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta 10561 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga 10621 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc 10681 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg 10741 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaaac actacctgga 10801 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct 10861 ggacaaggtg ctgagcgcct acaacaagca cagagacaag cctatcagag agcaggccga 10921 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt 10981 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct 11041 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg 11101 cgacgcggcc gcggagcaga aactcatctc tgaagaagat ctggaacaaa agttgatttc 11161 agaagaagat ctggaacaga agctcatctc tgaggaagat ctgtaataag gcgcgcctcc 11221 ttaatgggac ttgcagcctc ggtaccaaat tccagaaaag acacccgaaa gggtgttttt 11281 tcgttttggt cccacagaat gagcatcatg gctctagtcg acatcggaga agagtacggc 11341 tcttttaacc gcctcaagaa ccagataagt gaaatctagt tccaaactat tttgtcattt 11401 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc 11461 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc 11521 cacagcgggg catttttctt cctgttatgt ttgggcgctg cattaatgaa tcggccaacg 11581 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgacccgct 11641 gcgctcggtc gttcggctgc ggcgagcggt atcag //