LOCUS Exported 10327 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeted A to G in wheat plants or monocotyledons protoplasts . ACCESSION . VERSION . KEYWORDS PABE-6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10327) AUTHORS Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C TITLE Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. JOURNAL Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. PUBMED 29807545 REFERENCE 2 (bases 1 to 10327) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10327 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1975..6075 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(D10A) /note="generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" RBS 5988..5996 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 6088..6108 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6121..6141 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 6199..6375 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" primer_bind complement(6929..6945) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6929..6945) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6942..6964) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6953..6969 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6977..7007) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7022..7043 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7160..7177) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7331..7919) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7411..7430) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8090..8950) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8713..8732 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8951..9055) /gene="bla" /label=AmpR promoter ORIGIN 1 ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt 61 atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt 121 ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa ttaattctgt 181 ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca 241 tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat 301 gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg 361 tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct 421 ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt 481 taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat 541 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct 601 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca 661 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg 721 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aaagcttatg 781 tccgaggtgg agttctctca cgagtattgg atgaggcacg ctcttacact tgctaagaga 841 gcttgggacg aaagagaagt gccagttggc gccgttcttg tgcataataa tagggtgatc 901 ggcgagggtt ggaatagacc aattggaagg catgatccaa cagctcacgc agagattatg 961 gctctcagac aaggcggcct cgttatgcag aactacaggc tcattgacgc tacactctac 1021 gtgacactcg aaccttgcgt tatgtgcgcc ggagctatga ttcattctag gattggcagg 1081 gtcgtgtttg gagctaggga cgctaaaaca ggagccgccg gatctcttat ggacgtgttg 1141 catcatccag gcatgaacca tagggtggag attacagagg gcattcttgc agacgagtgc 1201 gctgctcttc tttccgattt cttcaggatg agaaggcagg agattaaggc ccagaagaag 1261 gctcagtctt ctacagatag cggaggatct tccggaggat ctagcggctc cgagacacca 1321 ggaacatccg aaagcgctac accagaatct agcggaggct cttccggagg atcttctgaa 1381 gtggagttct cccacgagta ttggatgagg cacgctctta cacttgctaa aagggctagg 1441 gacgaaaggg aagttccagt tggagctgtt ctcgtgctca ataacagggt gattggcgag 1501 ggttggaata gagccattgg actccatgat ccaacagctc acgcagagat tatggctctt 1561 agacaaggcg gcctcgttat gcagaattac agactcatcg acgccacact ctacgttacc 1621 ttcgaacctt gcgttatgtg cgccggagct atgatccatt ctaggattgg cagggtcgtg 1681 ttcggcgtta gaaacgctaa gacaggagct gcaggctctc ttatggacgt tcttcattac 1741 ccaggcatga atcatagagt ggagatcaca gaaggcattc ttgcagacga gtgcgcagct 1801 ctcctttgct atttcttcag gatgccgagg caagttttca acgctcagaa gaaggcccag 1861 tcttctacag attccggcgg atcttctgga ggatctagcg gctccgagac accaggaaca 1921 tccgaatccg ctacaccaga gtcttctgga ggatctagcg gaggatctct taaggacaag 1981 aagtactcga tcggcctcgc cattgggact aactctgttg gctgggccgt gatcaccgac 2041 gagtacaagg tgccctcaaa gaagttcaag gtcctgggca acaccgatcg gcattccatc 2101 aagaagaatc tcattggcgc tctcctgttc gacagcggcg agacggctga ggctacgcgg 2161 ctcaagcgca ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg ctacctgcag 2221 gagattttct ccaacgagat ggcgaaggtt gacgattctt tcttccacag gctggaggag 2281 tcattcctcg tggaggagga taagaagcac gagcggcatc caatcttcgg caacattgtc 2341 gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg 2401 gactccacag ataaggcgga cctccgcctg atctacctcg ctctggccca catgattaag 2461 ttcaggggcc atttcctgat cgagggggat ctcaacccgg acaatagcga tgttgacaag 2521 ctgttcatcc agctcgtgca gacgtacaac cagctcttcg aggagaaccc cattaatgcg 2581 tcaggcgtcg acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg gcgcctcgag 2641 aacctgatcg cccagctgcc gggcgagaag aagaacggcc tgttcgggaa tctcattgcg 2701 ctcagcctgg ggctcacgcc caacttcaag tcgaatttcg atctcgctga ggacgccaag 2761 ctgcagctct ccaaggacac atacgacgat gacctggata acctcctggc ccagatcggc 2821 gatcagtacg cggacctgtt cctcgctgcc aagaatctgt cggacgccat cctcctgtct 2881 gatattctca gggtgaacac cgagattacg aaggctccgc tctcagcctc catgatcaag 2941 cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtcag gcagcagctc 3001 cccgagaagt acaaggagat cttcttcgat cagtcgaaga acggctacgc tgggtacatt 3061 gacggcgggg cctctcagga ggagttctac aagttcatca agccgattct ggagaagatg 3121 gacggcacgg aggagctgct ggtgaagctc aatcgcgagg acctcctgag gaagcagcgg 3181 acattcgata acggcagcat cccacaccag attcatctcg gggagctgca cgctatcctg 3241 aggaggcagg aggacttcta ccctttcctc aaggataacc gcgagaagat cgagaagatt 3301 ctgactttca ggatcccgta ctacgtcggc ccactcgcta ggggcaactc ccgcttcgct 3361 tggatgaccc gcaagtcaga ggagacgatc acgccgtgga acttcgagga ggtggtcgac 3421 aagggcgcta gcgctcagtc gttcatcgag aggatgacga atttcgacaa gaacctgcca 3481 aatgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcacagt ctacaacgag 3541 ctgactaagg tgaagtatgt gaccgagggc atgaggaagc cggctttcct gtctggggag 3601 cagaagaagg ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcag 3661 ctcaaggagg actacttcaa gaagattgag tgcttcgatt cggtcgagat ctctggcgtt 3721 gaggaccgct tcaacgcctc cctggggacc taccacgatc tcctgaagat cattaaggat 3781 aaggacttcc tggacaacga ggagaatgag gatatcctcg aggacattgt gctgacactc 3841 actctgttcg aggaccggga gatgatcgag gagcgcctga agacttacgc ccatctcttc 3901 gatgacaagg tcatgaagca gctcaagagg aggaggtaca ccggctgggg gaggctgagc 3961 aggaagctca tcaacggcat tcgggacaag cagtccggga agacgatcct cgacttcctg 4021 aagagcgatg gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga cagcctcaca 4081 ttcaaggagg atatccagaa ggctcaggtg agcggccagg gggactcgct gcacgagcat 4141 atcgcgaacc tcgctggctc gccagctatc aagaagggga ttctgcagac cgtgaaggtt 4201 gtggacgagc tggtgaaggt catgggcagg cacaagcctg agaacatcgt cattgagatg 4261 gcccgggaga atcagaccac gcagaagggc cagaagaact cacgcgagag gatgaagagg 4321 atcgaggagg gcattaagga gctggggtcc cagatcctca aggagcaccc ggtggagaac 4381 acgcagctgc agaatgagaa gctctacctg tactacctcc agaatggccg cgatatgtat 4441 gtggaccagg agctggatat taacaggctc agcgattacg acgtcgatca tatcgttcca 4501 cagtcattcc tgaaggatga ctccattgac aacaaggtcc tcaccaggtc ggacaagaac 4561 cggggcaagt ctgataatgt tccttcagag gaggtcgtta agaagatgaa gaactactgg 4621 cgccagctcc tgaatgccaa gctgatcacg cagcggaagt tcgataacct cacaaaggct 4681 gagaggggcg ggctctctga gctggacaag gcgggcttca tcaagaggca gctggtcgag 4741 acacggcaga tcactaagca cgttgcgcag attctcgact cacggatgaa cactaagtac 4801 gatgagaatg acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc aaagctcgtc 4861 tccgacttca ggaaggattt ccagttctac aaggttcggg agatcaacaa ttaccaccat 4921 gcccatgacg cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa gtacccaaag 4981 ctcgagagcg agttcgtgta cggggactac aaggtttacg atgtgaggaa gatgatcgcc 5041 aagtcggagc aggagattgg caaggctacc gccaagtact tcttctactc taacattatg 5101 aatttcttca agacagagat cactctggcc aatggcgaga tccggaagcg ccccctcatc 5161 gagacgaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc 5221 aggaaggttc tctccatgcc acaagtgaat atcgtcaaga agacagaggt ccagactggc 5281 gggttctcta aggagtcaat tctgcctaag cggaacagcg acaagctcat cgcccgcaag 5341 aaggactggg atccgaagaa gtacggcggg ttcgacagcc ccactgtggc ctactcggtc 5401 ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctg 5461 ctggggatca cgattatgga gcgctccagc ttcgagaaga acccgatcga tttcctggag 5521 gcgaagggct acaaggaggt gaagaaggac ctgatcatta agctccccaa gtactcactc 5581 ttcgagctgg agaacggcag gaagcggatg ctggcttccg ctggcgagct gcagaagggg 5641 aacgagctgg ctctgccgtc caagtatgtg aacttcctct acctggcctc ccactacgag 5701 aagctcaagg gcagccccga ggacaacgag cagaagcagc tgttcgtcga gcagcacaag 5761 cattacctcg acgagatcat tgagcagatt tccgagttct ccaagcgcgt gatcctggcc 5821 gacgcgaatc tggataaggt cctctccgcg tacaacaagc accgcgacaa gccaatcagg 5881 gagcaggctg agaatatcat tcatctcttc accctgacga acctcggcgc ccctgctgct 5941 ttcaagtact tcgacacaac tatcgatcgc aagaggtaca caagcactaa ggaggtcctg 6001 gacgcgaccc tcatccacca gtcgattacc ggcctctacg agacgcgcat cgacctgtct 6061 cagctcgggg gcgactccgg cggcagccca aagaagaagc ggaaggtgtc tggaggttct 6121 cctaagaaaa agagaaaagt gtaggaattc ggtacgctga aatcaccagt ctctctctac 6181 aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa 6241 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta 6301 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt 6361 actaaaatcc agatctccta aagtccctat agatctttgt cgtgaatata aaccagacac 6421 gagacgacta aacctggagc ccagacgccg ttcgaagcta gaagtaccgc ttaggcagga 6481 ggccgttagg gaaaagatgc taaggcaggg ttggttacgt tgactccccc gtaggtttgg 6541 tttaaatatg atgaagtgga cggaaggaag gaggaagaca aggaaggata aggttgcagg 6601 ccctgtgcaa ggtaagaaga tggaaatttg atagaggtac gctactatac ttatactata 6661 cgctaaggga atgcttgtat ttatacccta taccccctaa taacccctta tcaatttaag 6721 aaataatccg cataagcccc cgcttaaaaa ttggtatcag agccatgaat aggtctatga 6781 ccaaaactca agaggataaa acctcaccaa aatacgaaag agttcttaac tctaaagata 6841 aaagatcttt caagatcaaa actagttccc tcacaccggt gacggggatc gcatgcgata 6901 tctcgagatc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 7021 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 7081 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 7141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 7201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 7261 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 7321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 7381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 7441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 7501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 7561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 7621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7741 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 9061 ttccccgaaa agtgccacct gccagtgcca agctaattcg agctcggtac ctgacccggt 9121 cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata 9181 ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact 9241 ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat 9301 ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta 9361 cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat 9421 ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat 9481 agactaattt ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta 9541 aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg 9601 actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg 9661 tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca 9721 gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg 9781 cctctggacc cctctcgatc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc 9841 atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct 9901 cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt 9961 cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg 10021 ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct 10081 tcaaggtacg ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc 10141 cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt 10201 tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt 10261 ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc 10321 gcagacg //