LOCUS Exported 10910 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of BRAF V600R. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-V600R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10910) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10910) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10910 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 3446..3455 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(5750..5774) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 5782..5800 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5825..5844) /label=T7 /note="T7 promoter, forward primer" promoter complement(5826..5844) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(5850..5870) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 5924..5965 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5986..6553 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6003..6555 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6147..6164) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6374..6393 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6567..7286 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6567..7283 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6612..6633) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6873..6892) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7220..7241 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7300..7888 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7353..7373) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7771..7782) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7963..8196 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8315..8333) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8401..8505 /gene="bla" /label=AmpR promoter CDS 8506..9366 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8724..8743) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9537..10125 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10026..10045 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10279..10296 /label=L4440 /note="L4440 vector, forward primer" primer_bind 10388..10407 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10536..10557 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10572..10602 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10610..10626 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10615..10637 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10634..10650 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10634..10650 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10700..10719 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg gcggcgctga gcggtggcgg tggtggcggc 3481 gcggagccgg gccaggctct gttcaacggg gacatggagc ccgaggccgg cgccggcgcc 3541 ggcgccgcgg cctcttcggc tgcggaccct gccattccgg aggaggtgtg gaatatcaaa 3601 caaatgatta agttgacaca ggaacatata gaggccctat tggacaaatt tggtggggag 3661 cataatccac catcaatata tctggaggcc tatgaagaat acaccagcaa gctagatgca 3721 ctccaacaaa gagaacaaca gttattggaa tctctgggga acggaactga tttttctgtt 3781 tctagctctg catcaatgga taccgttaca tcttcttcct cttctagcct ttcagtgcta 3841 ccttcatctc tttcagtttt tcaaaatccc acagatgtgg cacggagcaa ccccaagtca 3901 ccacaaaaac ctatcgttag agtcttcctg cccaacaaac agaggacagt ggtacctgca 3961 aggtgtggag ttacagtccg agacagtcta aagaaagcac tgatgatgag aggtctaatc 4021 ccagagtgct gtgctgttta cagaattcag gatggagaga agaaaccaat tggttgggac 4081 actgatattt cctggcttac tggagaagaa ttgcatgtgg aagtgttgga gaatgttcca 4141 cttacaacac acaactttgt acgaaaaacg tttttcacct tagcattttg tgacttttgt 4201 cgaaagctgc ttttccaggg tttccgctgt caaacatgtg gttataaatt tcaccagcgt 4261 tgtagtacag aagttccact gatgtgtgtt aattatgacc aacttgattt gctgtttgtc 4321 tccaagttct ttgaacacca cccaatacca caggaagagg cgtccttagc agagactgcc 4381 ctaacatctg gatcatcccc ttccgcaccc gcctcggact ctattgggcc ccaaattctc 4441 accagtccgt ctccttcaaa atccattcca attccacagc ccttccgacc agcagatgaa 4501 gatcatcgaa atcaatttgg gcaacgagac cgatcctcat cagctcccaa tgtgcatata 4561 aacacaatag aacctgtcaa tattgatgac ttgattagag accaaggatt tcgtggtgat 4621 ggaggatcaa ccacaggttt gtctgctacc ccccctgcct cattacctgg ctcactaact 4681 aacgtgaaag ccttacagaa atctccagga cctcagcgag aaaggaagtc atcttcatcc 4741 tcagaagaca ggaatcgaat gaaaacactt ggtagacggg actcgagtga tgattgggag 4801 attcctgatg ggcagattac agtgggacaa agaattggat ctggatcatt tggaacagtc 4861 tacaagggaa agtggcatgg tgatgtggca gtgaaaatgt tgaatgtgac agcacctaca 4921 cctcagcagt tacaagcctt caaaaatgaa gtaggagtac tcaggaaaac acgacatgtg 4981 aatatcctac tcttcatggg ctattccaca aagccacaac tggctattgt tacccagtgg 5041 tgtgagggct ccagcttgta tcaccatctc catatcattg agaccaaatt tgagatgatc 5101 aaacttatag atattgcacg acagactgca cagggcatgg attacttaca cgccaagtca 5161 atcatccaca gagacctcaa gagtaataat atatttcttc atgaagacct cacagtaaaa 5221 ataggtgatt ttggtctagc tacaaggaaa tctcgatgga gtgggtccca tcagtttgaa 5281 cagttgtctg gatccatttt gtggatggca ccagaagtca tcagaatgca agataaaaat 5341 ccatacagct ttcagtcaga tgtatatgca tttggaattg ttctgtatga attgatgact 5401 ggacagttac cttattcaaa catcaacaac agggaccaga taatttttat ggtgggacga 5461 ggatacctgt ctccagatct cagtaaggta cggagtaact gtccaaaagc catgaagaga 5521 ttaatggcag agtgcctcaa aaagaaaaga gatgagagac cactctttcc ccaaattctc 5581 gcctctattg agctgctggc ccgctcattg ccaaaaattc accgcagtgc atcagaaccc 5641 tccttgaatc gggctggttt ccaaacagag gattttagtc tatatgcttg tgcttctcca 5701 aaaacaccca tccaggcagg gggatatggt gcgtttcctg tccactagaa cccagctttc 5761 ttgtacaaag tggtgatatc caattaaccc tcactaaagg aagatgatag ttaagtgtaa 5821 gttaccctat agtgagtcgt attaccaccc aacttttcta tacaaagtgg ttgatatcca 5881 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa 5941 ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc 6001 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt 6061 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt 6121 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt 6181 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct 6241 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt 6301 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt 6361 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa 6421 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta 6481 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa 6541 aacacgatga taatatggcc acacatatgg tgagcaaggg cgaggagctg ttcaccgggg 6601 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 6661 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 6721 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 6781 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 6841 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 6901 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 6961 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 7021 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 7081 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 7141 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 7201 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 7261 tcggcatgga cgagctgtac aagtaaatcg atagatccta atcaacctct ggattacaaa 7321 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 7381 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 7441 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 7501 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 7561 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 7621 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 7681 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt 7741 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 7801 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 7861 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg 7921 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 7981 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 8041 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 8101 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 8161 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 8221 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta 8281 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag 8341 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 8401 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 8461 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 8521 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 8581 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 8641 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 8701 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg 8761 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 8821 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 8881 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 8941 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 9001 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 9061 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 9121 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 9181 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 9241 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 9301 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 9361 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 9421 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 9481 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 9541 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 9601 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 9661 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 9721 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 9781 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 9841 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 9901 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 9961 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 10021 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 10081 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 10141 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 10201 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 10261 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 10321 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa 10381 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 10441 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt 10501 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 10561 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 10621 taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt 10681 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 10741 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt 10801 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt 10861 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt //