LOCUS       Exported                9796 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  for stabilizing ICE in the presence of rapI expression.
ACCESSION   .
VERSION     .
KEYWORDS    pJMP1363
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 9796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..48
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1140..5243
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5253..5342
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     oriT            6512..6620
                     /note="incP origin of transfer"
     rep_origin      6889..7277
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(7578..8438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8201..8220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8439..8543)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      9463..9557
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      9660..9746
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaagtaccta ttccgaagtt cctattctct agaaagtata ggaacttcag agcgcttttg
       61 aagctgatgc tcgaggcgta gaggatctgg agctgtaata taaaaacctt cttcaactaa
      121 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat
      181 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc
      241 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg
      301 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat
      361 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag
      421 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat
      481 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag
      541 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac
      601 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg
      661 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata
      721 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat
      781 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg
      841 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag
      901 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
      961 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaaagac
     1021 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     1081 ttttctttct tatcttgata ataagggtaa ctattgccga tgataagctg tcaactagta
     1141 tggataagaa atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga
     1201 tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
     1261 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
     1321 cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
     1381 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
     1441 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
     1501 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
     1561 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
     1621 tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg
     1681 tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta
     1741 ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac
     1801 gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc
     1861 tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag
     1921 atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc
     1981 aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt
     2041 tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa
     2101 tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac
     2161 aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag
     2221 gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag
     2281 aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca
     2341 agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg
     2401 ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg
     2461 aaaaaatctt gacttttcga atcccttatt atgttggtcc attggcgcgt ggcaatagtc
     2521 gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag
     2581 ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa
     2641 atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt
     2701 ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt
     2761 caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg
     2821 ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt
     2881 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
     2941 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
     3001 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
     3061 acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac
     3121 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
     3181 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
     3241 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
     3301 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
     3361 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
     3421 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta
     3481 tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
     3541 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
     3601 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca
     3661 ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg
     3721 ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa
     3781 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
     3841 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
     3901 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
     3961 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
     4021 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
     4081 accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat
     4141 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
     4201 tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta
     4261 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
     4321 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
     4381 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac
     4441 agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg
     4501 ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt
     4561 attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta
     4621 aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact
     4681 ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat
     4741 atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac
     4801 aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc
     4861 attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc
     4921 agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta
     4981 ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac
     5041 caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc
     5101 ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag
     5161 aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg
     5221 atttgagtca gctaggaggt gacgcggccg cggagcagaa actcatctct gaagaagatc
     5281 tggaacaaaa gttgatttca gaagaagatc tggaacagaa gctcatctct gaggaagatc
     5341 tgtaataagg cgcgcctcct taatgggact tgcagcctcg gtaccaaatt ccagaaaaga
     5401 cacccgaaag ggtgtttttt cgttttggtc ccacagaatg agcatcatgg ctctagtcga
     5461 ccgggtaggg gcatagctta gataattgga aaagaggaaa aaagcttaat cttttttcga
     5521 aggttaagct ttttctttta tttataaaaa gtgaactaac tatcagaaag aaattatatt
     5581 aaattttatt tttttgttta aaaagtagat tatataaagg caagctaggt gggggaaaat
     5641 atgtttaaaa aagaaaaagt cacagaatac atttggacta tactaatacc aacaatcatc
     5701 acttttatca ttagttgggt tgggtcttat tacaatggta cttcgacagt tagtattgga
     5761 caacctacaa aagtttccgg tcagtatatc acgccaataa atataagtcc ctatcatgat
     5821 attaaggaat taagaataac ttttccgcaa aaactagatg taaaacaaat tagttcaaat
     5881 gagcctataa atgtaaaatc agataagaac aatataggag ttgaaagtaa ttccactttt
     5941 gagattgcga aaatcgttga aaataatagc gttcagttgc taattacaac acaaaaaaag
     6001 ttaaacgata aggaaattag aattgataaa aatggaaata acatttctgt aaattatgaa
     6061 tctcagattg ttaatcctgc aaaaaaacaa ttaatcaatc ttataattac gtcatctatt
     6121 tattttataa tgcttaatat actagcattg attatgaaca aaagatggga taagtattat
     6181 gcaaaaatga aaaatgaaat caaagaattt gaggataatg caaaagatct tgataaaaaa
     6241 tcaaagaaga aaagcgagga attatcggag ctgcgaaaga ccttgaacca agcgtttgag
     6301 gaaactgata ggataaaata tcatgagaag aaaaaacaaa tcctcctctt agctaagtta
     6361 aacgattata aaaaagaact aaccttttgg agaaatacaa taagaaaagt tctttatgaa
     6421 cttcctgatg gagataaaaa agcagataaa ctaataggga cagttacatc atctttaaaa
     6481 acgtacggta cagtcgaaaa gtaaagctta tcggccagcc tcgcagagca ggattcccgt
     6541 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
     6601 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
     6661 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
     6721 taatgacccc gaagcagggt tatgcagcgg aaagtatacc ttaaggaatc ccctgataac
     6781 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgca
     6841 ctccctgcct ctgtcatcac gatactgtga tgccatggct aattcccatg tcagccgtta
     6901 agtgttcctg tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac
     6961 atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc
     7021 ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat
     7081 tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat
     7141 gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt
     7201 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa
     7261 ctgctgatct tcagatcctc tacgccggac gcatcgtggc cggatcttgc ggccgcaaaa
     7321 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7381 accaatgctt aatcagtgag gcaccaataa ctgcctttga tcttttctac ggggtctgac
     7441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7681 ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca
     7741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8521 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcttctaa
     8581 ctacttattt taaagcagtc tggattgttt gggtaattca taaaaaaata aaagaaagaa
     8641 ggaggaatag agtttttctt ttttttgttt gcaatgttac tgtcaagtcg caaaagaatt
     8701 gctatgaccg aaaaaaaacc gcaaaggaat agatataagg tacctttttg caattcatct
     8761 ttgtaaaata aaggttattc tgacataata caattaatgt aaaaattcgc acaattttat
     8821 gtaaggatgg gggaattttc ttgcggggtg ttttcttaga taaagataaa attccgtacg
     8881 acttagtcac gaaaaagtta aatgaatggt atacatcaat aaaaaatgat caagttgagc
     8941 aagccgagat tataaaaaca gaagtagaga aagaattgtt aaacatggaa gaaaatcaag
     9001 atgccctgtt atattatcaa ctattagaat ttagacatga gataatgctg agttatatga
     9061 aatctaagga aatagaagat ctcaataatg cttatgagac tataaaagaa attgagaagc
     9121 aagggcaatt aactggcatg ttggaatact atttttactt ttttaagggt atgtacgagt
     9181 ttaggcgtaa agaattaatt tcagcgataa gtgcttatcg aatagctgaa tcaaagttgt
     9241 cagaagttga ggatgaaata gagaaagcag agtttttttt caaagtgtcc tatgtatatt
     9301 attatatgaa acaaacatac ttctccatga attatgcaaa tcgtgcactc aaaatattta
     9361 gagagtatga agaatatgct gtccagactg tgcgttgtca atttattgta gcaggaaact
     9421 tgatcgagtg aatcgataag ctagcttaat tagctgagct tggactcctg ttgatagatc
     9481 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
     9541 tttttattgg tgagaatcca agctagactg cgatgagtgg cagggcgggg cgtaattttt
     9601 ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc
     9661 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     9721 tgaacgctct cctgagtagg acaaatccgc cgctaggagc ttgcggcccg gacgatgagc
     9781 tcgaattggg gatctt
//
