LOCUS Exported 12595 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Bacterial genome editing plasmid with spectinomycin resistance marker. ACCESSION . VERSION . KEYWORDS pCpf1-sp SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12595) AUTHORS Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC TITLE Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120. JOURNAL ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437. PUBMED 30525474 REFERENCE 2 (bases 1 to 12595) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12595 /organism="synthetic DNA construct" /mol_type="other DNA" oriT 232..319 /label=RSF1010 oriT /note="origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" CDS 1558..2529 /codon_start=1 /gene="repB" /product="replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" /label=RSF1010 RepB /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM" CDS 3040..3879 /codon_start=1 /gene="repA" /product="replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" /label=RSF1010 RepA /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA" primer_bind 5227..5246 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter 5350..5379 /label=lac promoter /note="promoter for the E. coli lac operon" promoter 9303..9337 /label=J23119 promoter /note="bacterial promoter (Registry of Standard Biological Parts BBa_J23119)" CDS complement(9405..9701) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG" primer_bind 9551..9573 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 9565..9582 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 9566..9582 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 9592..9611 /label=T7 /note="T7 promoter, forward primer" promoter 9592..9610 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9658..9678) /label=T3 /note="T3 promoter, forward primer" promoter complement(9658..9676) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(9697..9713) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9697..9713) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9710..9732) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9721..9737 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9745..9775) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9790..9811 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." CDS 10521..11312 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 12091..12485 /label=RSF1010 oriV /note="replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)" ORIGIN 1 catgagccga tcctccggcc actcgctgct gttcaccttg gccaaaatca tggcccccac 61 cagcaccttg cgccttgttt cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg 121 ctgaatttcg gcattgattc gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc 181 cttgttgctc cccttaacca tcttgacacc ccattgttaa tgtgctgtct cgtaggctat 241 catggaggca cagcggcggc aatcccgacc ctactttgta ggggagggcg cacttaccgg 301 tttctcttcg agaaactggc ctaacggcca cccttcgggc ggtgcgctct ccgagggcca 361 ttgcatggag ccgaaaagca aaagcaacag cgaggcagca tggcgattta tcaccttacg 421 gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg ccaaggccga ctacatccag 481 cgcgaaggca agtatgcccg cgacatggat gaagtcttgc acgccgaatc cgggcacatg 541 ccggagttcg tcgagcggcc cgccgactac tgggatgctg ccgacctgta tgaacgcgcc 601 aatgggcggc tgttcaagga ggtcgaattt gccctgccgg tcgagctgac cctcgaccag 661 cagaaggcgc tggcgtccga gttcgcccag cacctgaccg gtgccgagcg cctgccgtat 721 acgctggcca tccatgccgg tggcggcgag aacccgcact gccacctgat gatctccgag 781 cggatcaatg acggcatcga gcggcccgcc gctcagtggt tcaagcggta caacggcaag 841 accccggaga agggcggggc acagaagacc gaagcgctca agcccaaggc atggcttgag 901 cagacccgcg aggcatgggc cgaccatgcc aaccgggcat tagagcgggc tggccacgac 961 gcccgcattg accacagaac acttgaggcg cagggcatcg agcgcctgcc cggtgttcac 1021 ctggggccga acgtggtgga gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg 1081 gccctgaaca tcgacaccgc caacgcccag atcatcgact tacaggaata ccgggaggca 1141 atagaccatg aacgcaatcg acagagtgaa gaaatccaga ggcatcaacg agttagcgga 1201 gcagatcgaa ccgctggccc agagcatggc gacactggcc gacgaagccc ggcaggtcat 1261 gagccagacc cagcaggcca gcgaggcgca ggcggcggag tggctgaaag cccagcgcca 1321 gacaggggcg gcatgggtgg agctggccaa agagttgcgg gaggtagccg ccgaggtgag 1381 cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg cactggaagc tatggctaac 1441 cgtgatgctg gcttccatga tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct 1501 cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg 1561 aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc 1621 ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc 1681 gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg 1741 tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag 1801 tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc 1861 ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg 1921 cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc 1981 cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgccggt 2041 tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg 2101 ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc 2161 aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg 2221 gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag 2281 tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc 2341 aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat 2401 tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc 2461 gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc 2521 agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa 2581 gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac 2641 aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc 2701 ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc 2761 gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga 2821 caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga 2881 gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga 2941 ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc 3001 cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca taagcctatc 3061 aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt gcccaacatg 3121 gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc catgctggcc 3181 ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg cgaactgccc 3241 accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca tcaccgcctg 3301 cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga cggcctgctg 3361 atccagccgc tgatcggcag cctgcccaac atcatggccc cggagtggtt cgacggcctc 3421 aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg gttccacatc 3481 gaggaagaaa acgccagcgg ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc 3541 gccgataccg ggtgctctat cgtgttcctg caccatgcca gcaagggcgc ggccatgatg 3601 ggcgcaggcg accagcagca ggccagccgg ggcagctcgg tactggtcga taacatccgc 3661 tggcagtcct acctgtcgag catgaccagc gccgaggccg aggaatgggg tgtggacgac 3721 gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc 3781 gctgatcggt ggttcaggcg gcatgacggc ggggtgctca agcccgccgt gctggagagg 3841 cagcgcaaga gcaagggggt gccccgtggt gaagcctaag aacaagcaca gcctcagcca 3901 cgtccggcac gacccggcgc actgtctggc ccccggcctg ttccgtgccc tcaagcgggg 3961 cgagcgcaag cgcagcaagc tggacgtgac gtatgactac ggcgacggca agcggatcga 4021 gttcagcggc ccggagccgc tgggcgctga tgatctgcgc atcctgcaag ggctggtggc 4081 catggctggg cctaatggcc tagtgcttgg cccggaaccc aagaccgaag gcggacggca 4141 gctccggctg ttcctggaac ccaagtggga ggccgtcacc gctgatgcca tggtggtcaa 4201 aggtagctat cgggcgctgg caaaggaaat cggggcagag gtcgatagtg gtggggcgct 4261 caagcacata caggactgca tcgagcgcct ttggaaggta tccatcatcg cccagaatgg 4321 ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg 4381 cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt 4441 gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca 4501 gcggctgtgt ggctggatcg accccggcaa aaccggcaag gcttccatag ataccttgtg 4561 cggctatgtc tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt 4621 gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg 4681 caagtacgac atcacccggc ccaaggcggc aggctgaccc cccccactct attgtaaaca 4741 agacattttt atcttttata ttcaatggct tattttcctg ctaattggta ataccatgaa 4801 aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact gagcgctgcc 4861 gcacagctcc ataggccgct ttcctggctt tgcttccaga tgtatgctct tctgctcctg 4921 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 4981 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 5041 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 5101 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 5161 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 5221 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 5281 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 5341 ctcgtgcact ttacacttta tgcttccggc tcgtatgtta ggaggtcttt atcatgtcaa 5401 tttatcaaga atttgttaat aaatatagtt taagtaaaac tctaagattt gagttaatcc 5461 cacagggtaa aacacttgaa aacataaaag caagaggttt gattttagat gatgagaaaa 5521 gagctaaaga ctacaaaaag gctaaacaaa taattgataa atatcatcag ttttttatag 5581 aggagatatt aagttcggtt tgtattagcg aagatttatt acaaaactat tctgatgttt 5641 attttaaact taaaaagagt gatgatgata atctacaaaa agattttaaa agtgcaaaag 5701 atacgataaa gaaacaaata tctgaatata taaaggactc agagaaattt aagaatttgt 5761 ttaatcaaaa ccttatcgat gctaaaaaag ggcaagagtc agatttaatt ctatggctaa 5821 agcaatctaa ggataatggt atagaactat ttaaagccaa tagtgatatc acagatatag 5881 atgaggcgtt agaaataatc aaatctttta aaggttggac aacttatttt aagggttttc 5941 atgaaaatag aaaaaatgtt tatagtagca atgatattcc tacatctatt atttatagga 6001 tagtagatga taatttgcct aaatttctag aaaataaagc taagtatgag agtttaaaag 6061 acaaagctcc agaagctata aactatgaac aaattaaaaa agatttggca gaagagctaa 6121 cctttgatat tgactacaaa acatctgaag ttaatcaaag agttttttca cttgatgaag 6181 tttttgagat agcaaacttt aataattatc taaatcaaag tggtattact aaatttaata 6241 ctattattgg tggtaaattt gtaaatggtg aaaatacaaa gagaaaaggt ataaatgaat 6301 atataaatct atactcacag caaataaatg ataaaacact caaaaaatat aaaatgagtg 6361 ttttatttaa gcaaatttta agtgatacag aatctaaatc ttttgtaatt gataagttag 6421 aagatgatag tgatgtagtt acaacgatgc aaagttttta tgagcaaata gcagctttta 6481 aaacagtaga agaaaaatct attaaagaaa cactatcttt attatttgat gatttaaaag 6541 ctcaaaaact tgatttgagt aaaatttatt ttaaaaatga taaatctctt actgatctat 6601 cacaacaagt ttttgatgat tatagtgtta ttggtacagc ggtactagaa tatataactc 6661 aacaaatagc acctaaaaat cttgataacc ctagtaagaa agagcaagaa ttaatagcca 6721 aaaaaactga aaaagcaaaa tacttatctc tagaaactat aaagcttgcc ttagaagaat 6781 ttaataagca tagagatata gataaacagt gtaggtttga agaaatactt gcaaactttg 6841 cggctattcc gatgatattt gatgaaatag ctcaaaacaa agacaatttg gcacagatat 6901 ctatcaaata tcaaaatcaa ggtaaaaaag acctacttca agctagtgcg gaagatgatg 6961 ttaaagctat caaggatctt ttagatcaaa ctaataatct cttacataaa ctaaaaatat 7021 ttcatattag tcagtcagaa gataaggcaa atattttaga caaggatgag catttttatc 7081 tagtatttga ggagtgctac tttgagctag cgaatatagt gcctctttat aacaaaatta 7141 gaaactatat aactcaaaag ccatatagtg atgagaaatt taagctcaat tttgagaact 7201 cgactttggc taatggttgg gataaaaata aagagcctga caatacggca attttattta 7261 tcaaagatga taaatattat ctgggtgtga tgaataagaa aaataacaaa atatttgatg 7321 ataaagctat caaagaaaat aaaggcgagg gttataaaaa aattgtttat aaacttttac 7381 ctggcgcaaa taaaatgtta cctaaggttt tcttttctgc taaatctata aaattttata 7441 atcctagtga agatatactt agaataagaa atcattccac acatacaaaa aatggtagtc 7501 ctcaaaaagg atatgaaaaa tttgagttta atattgaaga ttgccgaaaa tttatagatt 7561 tttataaaca gtctataagt aagcatccgg agtggaaaga ttttggattt agattttctg 7621 atactcaaag atataattct atagatgaat tttatagaga agttgaaaat caaggctaca 7681 aactaacttt tgaaaatata tcagagagct atattgatag cgtagttaat cagggtaaat 7741 tgtacctatt ccaaatctat aataaagatt tttcagctta tagcaaaggg cgaccaaatc 7801 tacatacttt atattggaaa gcgctgtttg atgagagaaa tcttcaagat gtggtttata 7861 agctaaatgg tgaggcagag cttttttatc gtaaacaatc aatacctaaa aaaatcactc 7921 acccagctaa agaggcaata gctaataaaa acaaagataa tcctaaaaaa gagagtgttt 7981 ttgaatatga tttaatcaaa gataaacgct ttactgaaga taagtttttc tttcactgtc 8041 ctattacaat caattttaaa tctagtggag ctaataagtt taatgatgaa atcaatttat 8101 tgctaaaaga aaaagcaaat gatgttcata tattaagtat agatagaggt gaaagacatt 8161 tagcttacta tactttggta gatggtaaag gcaatatcat caaacaagat actttcaaca 8221 tcattggtaa tgatagaatg aaaacaaact accatgataa gcttgctgca atagagaaag 8281 atagggattc agctaggaaa gactggaaaa agataaataa catcaaagag atgaaagagg 8341 gctatctatc tcaggtagtt catgaaatag ctaagctagt tatagagtat aatgctattg 8401 tggtttttga ggatttaaat tttggattta aaagagggcg tttcaaggta gagaagcagg 8461 tctatcaaaa gttagaaaaa atgctaattg agaaactaaa ctatctagtt ttcaaagata 8521 atgagtttga taaaactggg ggagtgctta gagcttatca gctaacagca ccttttgaga 8581 cttttaaaaa gatgggtaaa caaacaggta ttatctacta tgtaccagct ggttttactt 8641 caaaaatttg tcctgtaact ggttttgtaa atcagttata tcctaagtat gaaagtgtca 8701 gcaaatctca agagttcttt agtaagtttg acaagatttg ttataacctt gataagggct 8761 attttgagtt tagttttgat tataaaaact ttggtgacaa ggctgccaaa ggcaagtgga 8821 ctatagctag ctttgggagt agattgatta actttagaaa ttcagataaa aatcataatt 8881 gggatactcg agaagtttat ccaactaaag agttggagaa attgctaaaa gattattcta 8941 tcgaatatgg gcatggcgaa tgtatcaaag cagctatttg cggtgagagc gacaaaaagt 9001 tttttgctaa gctaactagt gtcctaaata ctatcttaca aatgcgtaac tcaaaaacag 9061 gtactgagtt agattatcta atttcaccag tagcagatgt aaatggcaat ttctttgatt 9121 cgcgacaggc gccaaaaaat atgcctcaag atgctgatgc caatggtgct tatcatattg 9181 ggctaaaagg tctgatgcta ctaggtagga tcaaaaataa tcaagagggc aaaaaactca 9241 atttggttat caaaaatgaa gagtattttg agttcgtgca gaataggaat aactaagaat 9301 tcttgacagc tagctcagtc ctaggtataa tgctagcgct gatttaggca aaaacgggtc 9361 taagaacttt aaataatttc tactgttgta gatgagagca ggtgtcagcc gctacagggc 9421 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 9481 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 9541 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac 9601 tcactatagg gcgaattggg taccgggccc cccctcgagg tcctccagct tttgttccct 9661 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 9721 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 9781 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 9841 cccacctgca aaagtctaag aactttaaat aatttctact gttgtagatg ctactattcc 9901 tgtgccttca gataattcag tctaagaact ttaaataatt tctactgttg tagatgtcta 9961 gagccttttg tattagtagc cggtctaaga actttaaata atttctactg ttgtagatta 10021 gcgatttatg aaggtcattt ttttgtctag ctttaatgcg gtagttggta ccgtcgatgg 10081 cagaaattcg atatctagat ctcatggatc cgcaatggca acaacgttgc gctcgactct 10141 agaagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc 10201 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga 10261 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt 10321 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt 10381 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg 10441 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc 10501 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 10561 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 10621 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 10681 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 10741 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 10801 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 10861 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 10921 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 10981 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 11041 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 11101 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 11161 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac 11221 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga 11281 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc 11341 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc 11401 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa 11461 attgggagat atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta 11521 atcgcaacat ccgcattaaa atctagcgag ggctttacta agctgatccg gtggatgacc 11581 ttttgaatga cctttaatag attatattac taattaattg gggaccctag aggtcccctt 11641 ttttatttta aaaatttttt cacaaaacgg tttacaagca taaagcttgc tcaatctgaa 11701 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag 11761 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc 11821 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag 11881 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct 11941 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg 12001 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc 12061 cagtgcccgt cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc 12121 tgcaataact gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc 12181 aggggcgggg gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca 12241 ctaggcgcgg ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc 12301 agaggccgga tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg 12361 gcagggggaa aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct 12421 ggagcgcttt tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg 12481 cagccccgca gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg 12541 gcagaccgaa caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgc //