LOCUS Exported 4741 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Plasma Membrane, Excitation: 590, Emission: 649. ACCESSION . VERSION . KEYWORDS mPlum-Farnesyl-5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4741) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 4741) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4741 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 168..471 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 472..675 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 626..646 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 720..1397 /codon_start=1 /product="far-red monomeric derivative of DsRed fluorescent protein (Wang et al., 2004)" /label=mPlum /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH STGA" primer_bind 1121..1140 /label=mCherry-F /note="mCherry, forward primer" primer_bind 1315..1338 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 1636..1757 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1673..1692) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1727..1746 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1764..2219) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1901..1922) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2113..2132 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2246..2350 /gene="bla" /label=AmpR promoter promoter 2352..2709 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2375..2395) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2560..2695 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2622..2641 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2744..3538 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2798..2817) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3408..3427 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3726..3745) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3770..3817 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4146..4734 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4635..4654 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 61 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag 121 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 181 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 241 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 301 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 361 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 421 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg 481 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc 541 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa 601 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc 661 tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gtggctacca 721 tggtgagcaa gggcgaggag gtcatcaagg agttcatgcg cttcaaggag cacatggagg 781 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 841 gcacccagac cgccaggctg aaggtgacca agggtggccc cctgcccttc gcctgggaca 901 tcctgtcccc tcagatcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc 961 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 1021 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct 1081 acaaggtgaa ggtgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga 1141 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg 1201 agatgaagat gaggctgagg ctgaaggacg gcggccacta cgacgccgag gtcaagacca 1261 cctacatggc caagaagccc gtgcagctgc ccggcgccta caagaccgac atcaagctgg 1321 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc 1381 gccactccac cggcgcctcc ggactcagat ctaagctgaa ccctcctgat gagagtggcc 1441 ccggctgcat gagctgcaag tgtgtgctct cctgagaatt ctgcagtcga cggtaccgcg 1501 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag 1561 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 1621 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 1681 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 1741 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 1801 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 1861 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 1921 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 1981 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 2041 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 2101 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 2161 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 2221 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 2281 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 2341 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 2401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2461 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 2521 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 2581 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 2641 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 2701 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 2761 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 2821 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 2881 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 2941 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 3001 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 3061 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 3121 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 3181 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 3241 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 3301 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 3361 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 3421 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 3481 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 3541 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 3601 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 3661 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 3721 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 3781 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 3841 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 3901 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 3961 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 4021 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 4081 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 4141 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 4201 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 4261 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 4321 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 4381 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 4441 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 4501 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 4561 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 4621 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 4681 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 4741 a //