LOCUS       Exported                4867 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  luciferase reporter plasmid with very low basal activity .
ACCESSION   .
VERSION     .
KEYWORDS    pBV-Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   He TC, Chan TA, Vogelstein B, Kinzler KW
  TITLE     PPARdelta is an APC-regulated target of nonsteroidal 
            anti-inflammatory drugs.
  JOURNAL   Cell. 1999 Oct 29. 99(3):335-45.
  PUBMED    10555149
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      131..140
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             137..1789
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(203..221)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1830..1951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1841..1860)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1895..1914
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2199..2216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2370..2958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2450..2469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3129..3989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3752..3771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3990..4094)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4121..4576
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4208..4227)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4418..4439
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4707..4755
                     /note="synthetic polyadenylation signal"
     misc_feature    4769..4860
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4809..4828
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgacagc tggacgtcga tatcgaattc
       61 gggtatataa tggatccggt atcgagatct gcgatctaag taagcttggc attccggtac
      121 tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
      181 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
      241 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga
      301 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
      361 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
      421 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
      481 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa
      541 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa
      601 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
      661 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat
      721 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc
      781 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
      841 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
      901 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag
      961 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
     1021 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     1081 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
     1141 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     1201 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     1261 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
     1321 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     1381 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
     1441 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
     1501 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga
     1561 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     1621 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     1681 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     1741 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg
     1801 gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac
     1861 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     1921 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     1981 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     2041 taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     2101 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     2161 aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc
     2221 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2281 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2341 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2461 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2521 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2581 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2641 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2701 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2761 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2821 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2881 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2941 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3001 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3061 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3121 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3181 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3241 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3301 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3361 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3421 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3481 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3541 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3601 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3661 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3721 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3781 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3841 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3901 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3961 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4021 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4081 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     4141 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4201 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4261 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4321 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4381 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4441 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4501 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4561 ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4621 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag
     4681 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg
     4741 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     4801 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     4861 atcgata
//
