LOCUS Exported 12781 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon. ACCESSION . VERSION . KEYWORDS pRS267 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12781) AUTHORS Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS TITLE A CRISPR Interference Platform for Efficient Genetic Repression in Candida albicans. JOURNAL mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 10.1128/mSphere.00002-19. PUBMED 30760609 REFERENCE 2 (bases 1 to 12781) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12781 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" CDS 3237..7340 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the N863A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 7353..7373 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 8671..8868 /label=TEF terminator /note="Ashbya gossypii TEF terminator" promoter complement(9469..9812) /label=TEF promoter /note="Ashbya gossypii TEF promoter" primer_bind complement(10766..10785) /label=T7 /note="T7 promoter, forward primer" promoter complement(10767..10785) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(10792..10809) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(10792..10808) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10801..10823) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(11037..11056) /label=F1ori-R /note="F1 origin, reverse primer" misc_RNA complement(11093..11167) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind 12623..12644 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataagca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt tcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggc agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag gggggaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggccga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgta agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta cctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagct cggaattaac cctcactaaa gggaacaaaa gctgaattgg agggtaccag 2221 agctattaag atcaccagcc tcgtttataa taaacttagt ctaatatttc ttttggacca 2281 aaatagaact gttctcaggt ggcaaaaaac agcttaaagt ctgtgttaaa aaaagatctc 2341 ggctatgcca atcaaaaagg agtgacatta ttatgtaaaa gaagaaatgt tgtaattccg 2401 cgaaacaaca attttaatgc ttactgtgcc gccaccaata tcctatggcc aaggggcctt 2461 taaacatgcc acggaaatat acagtcaaag taaacctact atgtctttag agccttcagg 2521 atcaattgtt tttaaatgaa tgactgcaaa actagaaaag tagcctctta cataagcaag 2581 tcatttgtac accgagagtt tagtaaccag tgaagcaagt atatctggca aacctgaaac 2641 ttatctgtga attataaagc tcataagtaa aaagtgtaga aaagaagata aggcacacca 2701 aatacaaatt tgcaacagta caacgaatat ttcagcgtca aaactagaga ataataaaga 2761 aaacgatctt ttcaaaaaga aaaaaccttt tagttttcct ttgttgttgt tgtgggtgtg 2821 tgctatttat attatatagt ttactcgtaa taccataaaa tattcggttt gattaggtta 2881 ttttaataag ctaatttgtt ttctaatcgt gtaatttatg ctgtgtatat taagtagtgt 2941 gtgtgcactg cccaaaaatg tttgttgttt atagtcggtt aaagagaaaa aagaaaaaaa 3001 gatccataca cacacgttaa ttagttgttc aacgtaatac acttatattt tgttcttatt 3061 tgctttcggt cgctgttctc accaggattt attgccaacg aaacaatttt tttttatata 3121 ttttcagatt tttctttttt tcctttcctt tccttttcta attttcactc ctggttttct 3181 ttctttctta gaaacattat ctcgatatta atattaaaaa aatataatca ttcaaaatgg 3241 ataaaaaata ctccataggt ttggccattg gaaccaattc agttggatgg gctgtaatca 3301 ctgatgaata taaggttcct tctaaaaagt tcaaagtttt aggaaatact gatagacact 3361 ccattaaaaa gaatttaatt ggtgccttgt tgtttgacag tggtgaaaca gctgaagcaa 3421 ctcgtttgaa aagaacagct agaagaagat ataccagaag aaagaacaga atttgttact 3481 tgcaagaaat cttttctaat gaaatggcaa aggttgatga ttcttttttt catagattgg 3541 aagaaagttt cttagttgaa gaagataaga aacatgaaag acatccaatc tttggtaata 3601 ttgtagatga agtggcttac cacgaaaaat atcctacaat ttatcatttg agaaaaaaat 3661 tagttgactc cactgataaa gctgacttaa gacttatata tttggcattg gctcacatga 3721 tcaagttcag aggtcacttc ttaattgaag gtgatttgaa tccagataat tccgatgtcg 3781 acaagttgtt tattcaattg gttcaaactt acaaccaact tttcgaggaa aatccaatca 3841 atgcctcagg tgtggatgct aaagctattt tatctgctag attgtcaaaa agtagacgtt 3901 tggaaaatct tattgcccaa ttgccaggtg aaaaaaaaaa tggattgttt ggaaacttaa 3961 ttgcattatc tttgggtttg accccaaatt tcaaatcaaa ctttgatttg gccgaggatg 4021 ctaaacttca attgtctaag gatacatacg atgacgattt agataatctt cttgcccaaa 4081 ttggtgatca atatgcagat ttattcttgg cagctaagaa cttgagtgat gcaattttgt 4141 taagtgacat tttgagagtc aacacagaaa ttacaaaagc tcctttatcc gcctctatga 4201 ttaaaagata tgatgaacac catcaagatt taaccttgtt gaaagctctt gtacgtcaac 4261 aattaccaga aaagtataaa gaaatattct ttgatcaatc taaaaatggt tacgctggtt 4321 atattgatgg tggtgctagt caagaagaat tttataagtt tatcaagcca attcttgaaa 4381 aaatggatgg tactgaggaa ttgttggtta agttgaatcg agaggattta cttcgtaagc 4441 aaagaacatt tgataacggt tctatccctc atcaaatcca cttaggtgag ttacacgcta 4501 tcttgagacg tcaggaagat ttttaccctt ttttgaaaga caatcgtgaa aaaattgaaa 4561 aaattttgac tttcagaatt ccttattacg tcgggccatt ggctagaggt aattctagat 4621 ttgcatggat gactcgtaaa tctgaagaga ctatcacccc atggaatttt gaggaagttg 4681 ttgataaggg agcctccgct caatcattca tcgagagaat gaccaatttc gacaaaaact 4741 tgccaaatga gaaggtgtta cctaaacatt ctttgttata tgaatatttc actgtttata 4801 atgaattaac aaaagtcaaa tacgtcactg aaggtatgag aaaaccagca ttcttaagtg 4861 gtgaacaaaa gaaagctatc gttgacttgt tattcaaaac taatcgtaaa gtcactgtca 4921 aacaacttaa agaggactat tttaagaaaa tagagtgttt cgattccgtt gaaatcagtg 4981 gggttgagga tagatttaat gctagtttag gaacttatca cgatttgttg aaaattatca 5041 aagacaaaga ttttttggat aatgaagaaa acgaagatat cttggaggac attgtattga 5101 ctttgacttt gtttgaggat agagaaatga tcgaagaacg tttaaaaact tacgctcact 5161 tattcgacga taaagtcatg aaacaattaa aaagaagaag atataccggt tggggaagat 5221 tatctagaaa gttgattaat ggtattagag ataagcaaag tggtaaaacc atcttagatt 5281 tccttaaaag tgatggtttt gcaaacagaa actttatgca gttgattcat gatgatagtt 5341 tgacctttaa ggaagatatt caaaaagctc aggtttctgg acagggtgat tcattgcatg 5401 aacatattgc caacttagca gggtctccag ctatcaaaaa gggaatttta caaactgtta 5461 aagttgttga tgagttggtg aaagttatgg gacgtcataa gcctgaaaat atagttatag 5521 aaatggctag agaaaatcaa acaactcaga aaggacaaaa gaattctcgt gaaagaatga 5581 aaagaataga agaaggaatt aaagagttgg gttctcaaat cttaaaagaa cacccagtcg 5641 aaaacaccca gttacagaac gagaagcttt acttgtacta tttgcaaaac gggcgtgaca 5701 tgtatgtaga tcaagagttg gatatcaata gactttcaga ttacgatgtc gatcacatag 5761 ttcctcagtc atttttaaaa gatgattcca ttgacaacaa agttttaaca agatctgata 5821 aggctagagg taaatcagac aatgtgccat cagaagaagt ggttaagaaa atgaagaact 5881 attggagaca attgttaaat gcaaagttga ttactcagag aaaatttgac aacttaacta 5941 aagctgaaag aggaggatta tccgaattag ataaagctgg attcataaaa cgtcagttgg 6001 ttgaaacaag acaaattact aaacatgtag cccaaatttt agattcccga atgaatacta 6061 aatatgatga aaatgacaaa ttaatcagag aggtcaaagt gatcacatta aagtcaaagt 6121 tggtgtcaga tttccgaaag gattttcaat tctacaaggt gagagagatc aacaattatc 6181 atcatgctca cgacgcatat ttgaatgcag tggtaggaac tgccttaata aagaagtatc 6241 ctaagttaga atccgaattc gtgtatggtg attataaagt ctacgatgtc agaaagatga 6301 tagcaaaatc agaacaagaa attgggaaag ccaccgcaaa gtatttcttt tactccaaca 6361 tcatgaattt ctttaagacc gaaatcacct tggctaacgg ggaaatcaga aaacgtcctt 6421 tgattgaaac aaatggagaa accggtgaaa ttgtgtggga taaggggaga gattttgcta 6481 ctgttagaaa agttttatca atgcctcaag ttaatattgt aaagaagact gaagttcaaa 6541 ctggtggttt ctctaaggaa tccattcttc caaaacgaaa ctcagacaag ttgatcgccc 6601 gaaaaaaaga ttgggatcct aagaaatacg gaggttttga ttctcctact gtcgcatatt 6661 ctgtcttggt cgtggctaaa gttgaaaagg gtaaatccaa aaaattgaaa tctgtgaaag 6721 aattgttagg tattaccatc atggagcgtt catcctttga gaaaaaccca attgactttc 6781 tcgaagcgaa gggctataaa gaagtgaaga aagatttgat tattaagttg ccaaagtatt 6841 ctctctttga acttgaaaat gggagaaaga gaatgttggc ttcagctgga gaattgcaaa 6901 aaggaaacga gttagctttg ccttcaaagt acgttaattt cttatactta gcatctcatt 6961 atgaaaagtt gaaggggtcc cctgaagaca atgagcaaaa gcaattattc gtagaacaac 7021 ataaacacta tttggatgag ataattgaac aaatctccga gttttctaaa agagttattt 7081 tggctgatgc aaatttggat aaagttttat ctgcttataa taagcatcgt gacaaaccaa 7141 ttcgagaaca agctgaaaat attatccact tgttcacatt gactaacttg ggagctccag 7201 ccgcgtttaa gtactttgac accaccattg accgtaagag atatacttca acaaaagaag 7261 tgttagacgc cacattgatt catcagtcca taactggttt atatgaaact agaatcgatt 7321 tgtcacaatt ggggggtgat agccgagcgg atcctaagaa aaaacgaaaa gtcagtggtg 7381 gtggtagtgg gtccgggtca ggaggttcat ccaagttagg aggttcagga gggagtatgg 7441 aacgagttag aatgatcaac gtacagcgtt tgttagaggc tgcagagttt ttggaaagac 7501 gagaacgaga atgcgagcat ggttacgcat cttccttccc atctatgcca tccccacgag 7561 ggagtggagg ggggtctggg tccggttctg gatctgggag ttcaaaatta gggggatccg 7621 ggggatctat ggaacgtgtc cgtatgatta atgtccaaag attattagaa gccgcagaat 7681 ttttagaaag acgagaaaga gaatgtgaac atggttatgc atcatcattt ccaagtatgc 7741 cttcaccaag aggttaaccc gggggatccg agtgaaattc tggaaatctg gaaatctggt 7801 tttgtattct tgttattctt ctttttgtta ttacatatat aacttgttac ttttttaaaa 7861 aaatctttgt ttattttata aatatataaa actaaattta agaaaaagag aaaaatgttt 7921 tatttgagag attgatattt tacttgaatt tagcttagct tttataaagt attattatgt 7981 aaaaaaacaa aacaaatata cattaaaaag ttaagactat aaaatagcca cccaaggcat 8041 ttctatatct tgttgttgtt gttttcatct tctgtatcag aggaacttat tttattattt 8101 tcgtcacggg tattttctct tgtttgatga ttcatcccat tcattccatc ataaaattct 8161 agaaggacca cctttgattg taaatagtaa taattaccac ccttatttaa ttatttattt 8221 aacttattta tttatttatt ctacatatat acaaatctaa taaagtgaaa atctccccct 8281 tcacatttat aattggccag tctttttcaa ataagcattc caaccagcat ttctatacct 8341 tttaccttca atatctggat ctcttccttt accaaacaat cctctaccaa caatgataat 8401 atcagttcca gtgctaacaa cttcatcaac agttctatat tgttgtccta atccatcacc 8461 tttatcatct aatccaactc caggtgtcat aataagccaa tcaaatcctt cttcttggcc 8521 acccatatca cgttgggcaa taaatccaat aacaaattcc ttatcggatt tagcaatttc 8581 aacagttttt tgagaatatt ctccatagta ccgaattcga gctcgtttaa acgtcgacac 8641 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt 8701 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg 8761 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa 8821 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac tgactcgagt 8881 tattatggac atggcataga catatacaaa gcttgttcac catcggaagc agtaccatcg 8941 tataaagcag tatccaaacc acacaaagtg aaacccattc ttctataagc atgaatagct 9001 ggagcattaa cattggtaac ttccaaccac aaatgaccag cacctctttc tctggcgaat 9061 tcagtagcca aacccatcaa agctctacca acaccatgac ctctatgttc tggagcaact 9121 tcaatatctt caacagtcaa tcttctgttc caaccagaat aagaaacaac aacgaaacca 9181 gccaaatcac catcatcacc ataagcaacg aaagttctag aatctggatc accatcttca 9241 ccagcatcgg attcatcatc ggattcatca tctgggaaaa ccttagtcaa tggtggatca 9301 actggaactt ctctcaaagt gaaaccatca ccagtagcag taactctaaa aacagtatcg 9361 gtagtgaaag aaccatccaa agcttcaata gcttcagcat cacctggaac agaagttctg 9421 tatctataag cagtatcatc caaagtagta gacataattg taggatccgg ttgtttatgt 9481 tcggatgtga tgtgagaact gtatcctagc aagattttaa aaggaagtat atgaaagaag 9541 aacctcagtg gcaaatccta accttttata tttctctaca ggggcgcggc gtggggacaa 9601 ttcaacgcgt ctgtgagggg agcgtttccc tgctcgcagg tctgcagcga ggagccgtaa 9661 tttttgcttc gcgccgtgcg gccatcaaaa tgtatggatg caaatgatta tacatgggga 9721 tgtatgggct aaatgtacgg gcgacagtca catcatgccc ctgagctgcg cacgtcaaga 9781 ctgtcaagga gggtattctg ggcctccatg tcgctggccg ggtgacccgg cggggacgag 9841 gcaagcttga ttgctaatga tcccactgat gataattcag ctaacatcaa taaccctctt 9901 ggctcttggt tggtggtggt ttctttagct ccctgtttta atccttcaac cactccattc 9961 ccagtgacac catgagcatt ggtaatatct gcccaactac taattttata aactccacca 10021 atatattgtt tctttacggt attaccaata tcagcaaatt ttctatcttc aaaaatcata 10081 aattgatgtt tacgtgaaag ttctaataat ggttcaatag tggattcata ggaaaaatca 10141 ttgattatat caatatgagt cttgattaag catacataag gacctaattt atcaattaat 10201 tcaaggaatt ccttagtggt atcaacgtca attgatgcac ataaattggt tttcttcagt 10261 tccattaatc gaaataatcg ctgtgctact ggtgaggcat gagtttctgc tctctcacta 10321 taggtcttag tgttgactgt catatctttt gtttgctatt tctatttcta tttctttctt 10381 caactgtaaa aggggcggta tataaaccag tgtgtatggg gttgttgctc aggaattgat 10441 caatgtttaa aagaatggtt gaaatcatca ttgaagaaca aaaaaaaaga ttttttcttt 10501 cattatgtta tctgtataat atgcaggaaa taagattgca aaaaaatagc cgtttttttg 10561 gatactatca aacaagagga atgactaata cagaattagt gttacgaatc aatggcacta 10621 cagcaacttt caacaaaata atctacgttc aaaatataat atatactatt aaataaaatc 10681 aaataataaa ataattttaa agaaagtagt accagctctt ttttttgttt ccgtttatac 10741 catccaaatc aattcctatt agtcgcccta tagtgagtcg tattacaatt cactggccgt 10801 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 10861 acatcccccc ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 10921 acagttgcgt agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 10981 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 11041 tttcgctttc ttcccttcct ttctcgccac gttcgccggc aaaaaaaagc accaccgact 11101 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct 11161 ctaaaacgtg gtctccgttg gttgccatcg aaattaaaaa tagtttacgc aagtctcgaa 11221 cttccaccac aatttaattg taagaacgct gcgaaaacat attctccaat cctattgggc 11281 cgaagtttca gctatgccaa aggcatcaaa ttttttttta taaaattttc cacttttcca 11341 taccagtgaa attatcacgt gatggagtga gtatctataa tatttgattg tttctttgat 11401 gaggcaatac tcgagtatag ttattgacca atgactttta ccggctaaac aagtggtatt 11461 caagcacaat tcagttttct ggatctctaa taattgcaat tgcaattgct tcacatactc 11521 tgtttataat tcagtttctt tctcttgatg ttactgatat attaagttaa taaataccaa 11581 taactttttt tcaaaaatct tctattttcc tttcaattga ttatttaaac acgaatcgtt 11641 aataagctgt gattgctttt tctttttctt tttcttttct ttgttcaagt ttgaaaaaag 11701 taaaaaaaaa ggtttctctt gttcgatgta gaattcagtg ggttatagtt gtaatgatat 11761 atggaataaa aagaaagaaa gaaagagaag agaaagagaa gaagagaaga attaagtaaa 11821 cgtgtattag aatttaaaaa ttaaaattca tgataatctt taataataat taactaagaa 11881 tcacaaaatt gaaaatttga aagaaaaaag atcactccaa ggtcgtgtca agtgactaga 11941 aattcttaat taaaatagtt ataacaaaaa aagaaatttg tatataacaa ttaagtaaaa 12001 gaaaaaaaag caattcgctt tgtataataa tacccagaaa aatatattca tatattcata 12061 aatcaaacat gaaatgaaga atgctgaatc acttgatagg atttagttcc attatggtac 12121 taagaagtaa tattacttct atgaaaaggt tgaatccgag atcaaaattc atcttttcat 12181 atactctgga gaaaaaaaaa aaagtaattg tagtaagaat gacaagtatc agttgagaaa 12241 aaaagtaaca aagagaaagc tcggaggagg ctccccaaag gttttatcac caatggtggt 12301 accactaacc caagaacaga aaaagcattg atattttgaa aaaatacaat actcatcttt 12361 taacccgaac cttttcttgt cctttaaaac ttttatctca tatctgtcca aagtcatatt 12421 gtcttactac tttctctcct tcatcgtcct tccggctttc cccgtcaagc tctaaatcgg 12481 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 12541 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 12601 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 12661 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 12721 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 12781 t //