LOCUS Exported 6874 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS MMTV-SV40-Bssk SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6874) AUTHORS Ornitz DM, Moreadith RW, Leder P TITLE Binary system for regulating transgene expression in mice: targeting int-2 gene expression with yeast GAL4/UAS control elements. JOURNAL Proc Natl Acad Sci U S A 1991 Feb 1;88(3):698-702. PUBMED 1846961 REFERENCE 2 (bases 1 to 6874) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6874 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 24..43 /label=T7 /note="T7 promoter, forward primer" promoter 24..42 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 271..290 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" intron 3416..3481 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 3611..3631 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 3903..4037 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3940..3959) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3994..4013 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4034..4050) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4034..4050) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(4087..4105) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4126..4142) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4126..4142) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4139..4161) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4150..4166 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4174..4204) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4219..4240 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4357..4374) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4528..5116) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4608..4627) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5287..6147) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5910..5929 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6148..6252) /gene="bla" /label=AmpR promoter rep_origin complement(6278..6733) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6415..6436) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6627..6646 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6860..8 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6874..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccgggc 61 cccccctcga ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag 121 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc 181 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag 241 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac 301 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcccaatgat 361 agagatttta ctgctctagt tccccataca gaattgtttc gcttagttgc agcctcaaga 421 tatcttattc tcaaaaggcc aggatttcaa gaacatgaca tgattcctac atctgcctgt 481 gttacttacc cttatgccat attattagga ttacctcagc taatagatat agagaaaaga 541 ggatctactt ttcatatttc ctgttcttct tgtagattga ctaattgttt agattcttct 601 gcctacgact atgcagcgat catagtcaag aggccgccat acgtgctgct acctgtagat 661 attggtgatg aaccatggtt tgatgattct gccattcaaa cctttaggta tgccacagat 721 ttaattcgag ccaagcgatt cgtcgctgcc attattctgg gcatatctgc tttaattgct 781 attatcactt cctttgctgt agctactact gctttagtta aggagatgca aactgctacg 841 tttgttaata atcttcatag gaatgttaca ttagccttat ctgaacaaag aataatagat 901 ttaaaattag aagctagact taatgcttta gaagaagtag ttttagagtt gggacaagat 961 gtggcaaact taaagaccag aatgtccacc aggtgtcatg caaattatga ttttatctgc 1021 gttacacctt taccatataa tgcttctgag agctgggaaa gaaccaaagc tcatttattg 1081 ggcatttgga atgacaatga gatttcatat aacatacaag aattaaccaa cctgattagt 1141 gatatgagca aacaacatat tgacacagtg gacctcagtg gcttggctca gtcctttgcc 1201 aatggagtaa aggctttaaa tccattagat tggacacaat atttcatttt tataggtgtt 1261 ggagccctgc ttttagtcat agtgcttatg attttcccca ttgttttcca gtgccttgcg 1321 aagagccttg accaagtgca gtcagatctt aacgtgcttc ttttaaaaaa gaaaaaaggg 1381 ggaaatgccg cgcctgcagc agaaatggtt gaactcccga gagtgtccta cacctagggg 1441 agaagcagcc aaggggttgt ttcccaccaa ggacgacccg tctgcgcaca aacggatgag 1501 cccatcagac aaagacatat tcattctctg ctgcaaactt ggcatagctc tgctttgcct 1561 ggggctattg ggggaagttg cggttcgtgc tcgcagggct ctcacccttg actcttttaa 1621 tagctcttct gtgcaagatt acaatctaaa caattcggag aactcgacct tcctcctgag 1681 gcaaggacca cagccaactt cctcttacaa gccgcatcga ttttgtcctt cagaaataga 1741 aataagaatg cttgctaaaa attatatttt taccaataag accaatccaa taggtagatt 1801 attagttact atgttaagaa atgaatcatt atcttttagt actattttta ctcaaattca 1861 gaagttagaa atgggaatag aaaatagaaa gagacgctca acctcaattg aagaacaggt 1921 gcaaggacta ttgaccacag gcctagaagt aaaaaaggga aaaaagagtg tttttgtcaa 1981 aataggagac aggtggtggc aaccagggac ttatagggga ccttacatct acagaccaac 2041 agatgccccc ttaccatata caggaagata tgacttaaat tgggataggt gggttacagt 2101 caatggctat aaagtgttat atagatccct cccttttcgt gaaagactcg ccagagctag 2161 acctccttgg tgtatgttgt ctcaagaaga aaaagacgac atgaaacaac aggtacatga 2221 ttatatttat ctaggaacag gaatgcactt ttggggaaag attttccata ccaaggaggg 2281 gacagtggct ggactaatag aacattattc tgcaaaaact tatggcatga gttattatga 2341 atagccttta ttggcccaac cttgcggttc ccagggctta agtaagtttt tggttacaaa 2401 ctgttcttaa aacgaggatg tgagacaagt ggtttcctga cttggtttgg tatcaaaggt 2461 tctgatctga gctctgagtg ttctattttc ctatgttctt ttggaattta tccaaatctt 2521 atgtaaatgc ttatgtaaac caagatataa aagagtgctg attttttgag taaacttgca 2581 acagtcctaa cattcacctc ttgtgtgttt gtgtctgttc gccatcccgt ctccgctcgt 2641 cacttatcct tcactttcca gagggtcccc ccgcagaccc cggcgtagag gatccgcacc 2701 cttgatgact ccgtctgaat ttttggtttc agtttggtac cgaagctgcg cggcgcgtct 2761 gcttgttact tgtttgactg ttggaattgt ttgtcttctt tgtgacctga ctgtggtttt 2821 ctggacgtgt tgtgtctgtt agtgtctttt tgacttttgt ttcgtgtttg aatttggact 2881 gacgactgtg tttaaaatct tagaccgacg actgtgtttg aaatcatgaa actgtttgct 2941 ttgttcgtcg aagagtttta cttggtcccc ttaacgctta gtgagtaaga aacttaattt 3001 tgtagacccc gctctagtgg cagtgtgttg gttgatagcc aaagttaatt tttaaaacat 3061 agtgttttgg gggttgggga tttagctcag tgatagagct cttgcctagc aagcgcaagg 3121 ccctgggttc ggtccccagc tctgaaaaaa aggaaagaga aacaaaacaa aaacatatag 3181 tgttttatct gtgcttatgc ccgcagcccg agccgcaccc gccgcggacg gagcccatgc 3241 gcgggcccag tcggcgcccg tccgcgcccc cgccctgccc cggccccggc ccccaagctt 3301 gatatcgaat tcctgcaggt cgcggccgcg actctagagg atctttgtga aggaacctta 3361 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa 3421 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta 3481 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa 3541 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca 3601 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt 3661 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta 3721 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta 3781 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 3841 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 3901 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 3961 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 4021 cttatcatgt ctggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt 4081 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc 4141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 4201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 4261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 4321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 4381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 4441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 4501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 4561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 4621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 4681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 4741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 4801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 4861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 4921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 4981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 5041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 5101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 5161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 5221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 5281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 5341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 5401 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 5461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 5521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 5581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 5641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 5701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 5761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 5821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 5881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 5941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 6001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 6061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 6121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 6181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 6241 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg 6301 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 6361 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 6421 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 6481 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 6541 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 6601 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 6661 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 6721 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6781 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6841 tgggtaacgc cagggttttc ccagtcacga cgtt //