LOCUS       Exported               14725 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Making Tet1 conditional knock out.
ACCESSION   .
VERSION     .
KEYWORDS    mTet1 flox donor
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14725)
  TITLE     The Molecular and Functional Foundations of Conducive Somatic Cell 
            Reprogramming to Ground State Pluripotency
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14725
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    4298..4331
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    4750..4783
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    4826..5050
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5039..5056
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5088..5891)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5199..5218)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5809..5828
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5905..6404)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5953..5972)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6346..6369
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    6425..6458
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    6475..6508
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gap             7431..7465
                     /estimated_length=35
     regulatory      10855..10864
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        10908..11012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11013..11873
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11231..11250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12044..12632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12533..12552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        12910..12972
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             13051..14181
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(14179..14198)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14223..14271
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     14703..14719
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 actcagactg ggaaaagagg taaactcttt caagtgggac aagtttgcag tggttcattt
       61 tcaggactaa gaaagaattg cctttgctta gccacattta cttctgttct atccatgtca
      121 aatacagttc aacctagagg gtgcatagac agaccgtaaa atcacgcaca gccgcagtga
      181 tggtgagcga atccacacct tctgcatctt gggctaggaa aggtgtgccc acatgaggag
      241 attctatagc ctggctgctg aggtggttga tcagggatat ataatcttgc ttactaaaga
      301 tttcgtgggg gttacggagg tgcctcagca ggcaaagcat ttgtacaacc ctgccccctg
      361 agttctatgt ccaggagctg tgataaacag tctcacatct ccacacatat gacaggaata
      421 ataaaatatc tctttaatta aaattatttg taaattttgt tctggaaggg ctgctttttt
      481 gttgtttttg agaggatgtg gtggcacctg tcattgcaac tttttaggga gctgaatgag
      541 ggagaagggt tttgagtcta gtctctattt tctagttaga atgtgcattc ctccaataca
      601 aacaaacaaa catggagagg ggctagagaa attcctgagc agtttaaggc ttgggcagga
      661 cacaagttca cttcctatca gtgtctccag tagctcacaa ccatcacctg tacctcctcc
      721 accctagaag atcctccgat ccttctttct ttctttcttt ctttctttct ttctttcttt
      781 ctttctttct ttggtttttt gagacagggt ttccctgtat agctctggct attctggaac
      841 tcactttgta gaccaggctg gcctcgaact cagaaatctg cctgcctctg cctcccgagt
      901 gctagaatta aaggcgtgca ctaccacgcc aggctaagat cctcctttct ctactggtca
      961 ctgcagtcac gcagactcgc acagactcct cctcaccctc cagacagggt ttctctgtat
     1021 aaccctgact gtcctgtaac tctctgtaga ccaggctatc ctcaaactca gagactttcc
     1081 tgcctctgcc tcagtgctag gattagggca tgtgcagctg ccaccatcac cacacagtga
     1141 tatgatattt ctgtaaaggt gtaggagcta gaaaaatgtt tcagaggtta agaacaaaat
     1201 aaacgcaaaa aaagaagaga gtacagattc tgttcttgaa gtttatctgc ctcatcaaac
     1261 ccctaattat aaagtgggct taataatata tatactgact ggatgtcttc aatgcaggtt
     1321 tagttattaa agtgcactga tgccagagat ggcccaaatt ctcaagatct gcttgaacca
     1381 aagggaatga gtatttgtcc acctagatgg aggctttttg agagtggctt gctaaatgag
     1441 agctgcaggc tgcgggtgta attgactgga aaggatggag gtagtggtac agactcatct
     1501 ttggccactt agaaagttca aaatcagaat taacatacat ttcttgattg agagaacatc
     1561 cacgcagagt tgtagagtag gactgtctta cttaactaga ccctgggcaa ataacctcta
     1621 cctgccattt cttttaaagc ttaagattac aataaattac ttttctgtat atctttttga
     1681 agtgaaaaat agattttcat taaaaataaa aaattagaaa caaacaaaca aacaaaaaat
     1741 aaaaaataag agcctgtgta tttctgggct gatctaggtt ttggagtgtg gggttatcgg
     1801 gttccccata caaagtcaca caaagacagg ccaggcagcc cattactgaa cacggaatgc
     1861 tatgctttcg ggtcagtggt tctcaacctg tgggtcttga cctcttaaat caaacttccc
     1921 tttcacaggg atgcctaaga ccatcagaaa tatcagatat tcattacatt cataactaac
     1981 aaaattatag tttataaagt agcaatgaaa ataattccac accgggtgtg gtggtgtatg
     2041 cctttaaacc cagcacttgg gaggcagagg caggcggatt tctgagttcg aggccagcct
     2101 ggtctacaga gtgagttcca ggacagccag ggctacacag agaaaccttg tctcgaaata
     2161 acaaaaacaa aaacaaaaaa caagaaaaga atcccatggc tagaggccgc cctaagttga
     2221 ggaactgtat tattaaaggg ttgcagcatt aagaaagctg agaaccacag ttttaggtga
     2281 ttttttttct gtgttcttaa acagacaaac aaaaactccg gttatctgag taacatcact
     2341 gtagctgagg gccttgcttt caggatggta tcaaatagtc aaatagaata agccatttct
     2401 gggtcagtgc ttgtcaaaat tagcagtatt gatacactaa acctaatccc actgtgtgta
     2461 gtttttcaca gtatccctgg cccctttcca tcagatggaa gccagagtga atgctcatat
     2521 ctatacttga cagaccccga agtaaccgaa actattgcca aatgtcccta tggtcagaat
     2581 tacccgattc taaatcacta gcaaaaaata aagtctcttc cacatgtctt actgaaaaat
     2641 agactggaga gatgactcag tagttaagag cacttgttgg tcttccagag gacccaggtt
     2701 caatttcaag cacccacatg gaagctcata accttttata actttcattg cattggatcc
     2761 aacaccttca caggcgtctg cactcctgag tgcatatcca catgcaaaca tacaaaccta
     2821 cagataatta aagtaaggac tgagtttgct ttggggtcat ctcttgcttt ttcttttttt
     2881 aaagactttt ttttgctcgc ttcagacaca ccagaagagg gcgtcagatc cgcttacaga
     2941 tggttgtgag ccaccatgtg cttgctggga tttgaactca ggacctctgg aagagcagcc
     3001 agtgctctta accgtagagc catctctgca gcctccagct ttctttcttt tttttttttt
     3061 ttttaattgc aaatattctc ctgcttgctc taatgtgtcc actcccccgg ccccaacccc
     3121 aactccaact gcatcactag caattctact agctacagcc ttgtatgtgc taggaaaaca
     3181 ctccacattt gagctacatt acatcttgaa accagaaaat tgactcgtaa aagcaccatg
     3241 gccagaaaat atataaaata cagagaggcc aaactaaaca tactattccc aaatttgaca
     3301 aaaattcaaa acaaacaggt tgtggaggtg tgtgccttgt agacaattaa ggatagcctg
     3361 gactaagata ttataagact gttcctcagc agtgtgtttg aaggatagcc tgggctacga
     3421 agcatgactg atttcctgtt gtcctattcc catagtgtct ccagctcttt tttaggatgc
     3481 aacattattg ttagtttaac ttatctacat ttacagaatt taggtcttta cacactttcc
     3541 aagtggactt tttgttgtgc atttataaca ggacattagt tagtgtataa ggcaggctgg
     3601 tcttgacccc taaaacagtc ttccagttct ccttcccagg cattggggtt acagctttgc
     3661 actcccttgg ttttgactgt tggaggctgg cctcagactc attgtgtagt ccaggcccca
     3721 cacccctcca ccttccagat acgtgaatat gtacacttta acgttctgtt ctgcctttaa
     3781 aaattgcagt gatactaaac gtgtctagtt gttgttctaa aaacgattgc atttacctct
     3841 aactgattcc ctttatattt acctacaact gattcccttc gtgcaggtgt taaagttctg
     3901 tcttttgata ataccaataa ttgtaacgtg taacttcctg gtacatgttt acgatgagta
     3961 cgtagttgtg cacatgtagt agggtgaatt gttaccagac tcactttcag tagggtatgg
     4021 aagctaagct tctcagacta gtgctcttcc ttaacgttgt tatagaaacc tacaaacgct
     4081 ggagagggag ccaggtgtga ggccagtggg ggcaggagtg tcaggttcaa ggccatcctt
     4141 ggatacctag taagagtgtc ttgaggttct actaagacct catcttacta agtttagtca
     4201 tattaataaa aaagctggga gaataaatac gccccccccc tttaaatttt tgttctctcc
     4261 tctgactgct agaagaaatg ttaactctag gagatctata acttcgtata gcatacatta
     4321 tacgaagtta taagcttaag agccagaagt ccaatgccag cccctgttgt cttatacgtt
     4381 aaaagttgaa actaatccaa acaaagagaa caaagcccct gtgctatttg taaaactggg
     4441 tctaataatc tgattccttc cacagatgga ggtacacaaa aagaaaaagg cccatattat
     4501 acacaccttg gggcaggacc aagtgtggct gctgtcaggg agctcatgga gactaggtga
     4561 ggaactctgc ttcccgctaa cccattcttc ccggtgacct ggctcaaggc agagaaaagg
     4621 gcttggagtg gtcctgaggc gaatgaagac ggtcaggagt gtgagttgga tggttcttaa
     4681 ggctggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgcccttg
     4741 cgtggtaccg aagttcctat tctctagaaa gtataggaac ttcctcgagg ggcgcgcccc
     4801 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta
     4861 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     4921 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     4981 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     5041 gaaggcacag tcgaggctga tcagcgagct ctagagaatt gatcccctca gaagaactcg
     5101 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     5161 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     5221 atgtcctgat agcgatccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     5281 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     5341 ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     5401 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     5461 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     5521 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     5581 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     5641 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc
     5701 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     5761 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     5821 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatg
     5881 gccgatccca tattggctgc aggtcgaaag gcccggagat gaggaagagg agaacagcgc
     5941 ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc
     6001 cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc ccagcctctg
     6061 agcccagaaa gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc ctacccgctt
     6121 ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta cttccatttg
     6181 tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt
     6241 agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt
     6301 ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa
     6361 agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtagg gcgcgccggg
     6421 atccgaagtt cctattctct agaaagtata ggaacttcat cgatcctaga attcataact
     6481 tcgtatagca tacattatac gaagttatcc gcggcaatag gcccagtcct cctgagtcct
     6541 cccaggagtg gggccctgga gacatgccct cactcggcag gcctatttga tcaatcagga
     6601 agagtgtttt cctactactg tacagtgata gaaagagctg agatgactcc cacccatgct
     6661 ttaaccctaa ctcgcccgtc ttccgagact tgtgttgagg tcaggctatt tttatttaga
     6721 gtgaccctta tgtaaacaga attgggtaac aaaatcagac tgccacgtaa cagctggttt
     6781 tagccaatgt aaacgataac tgtttcttct tttcttttta aaaaaaaaag aaaactgatt
     6841 catgtgtata ttcaatgcat ttagatgtga aaacatggag acttcgagtt cggatggtca
     6901 ctaattagtc ccaccctgta ctgtgttctg taccaggttt ggccagaagg ggaaggcaat
     6961 ccggattgag aagatagtgt tcacggggaa ggaagggaag agctctcagg gctgcccggt
     7021 cgccaagtgg gtgagtgctg ccagcagact tcgattttct tggtacttta gaaaatggat
     7081 ttttccaggg cattaaatta gaaggttttg ctttgcaaaa atggagacca gaaaactttt
     7141 catgcccaca gggagatggt ggagaagctg tgcgtctttc ctgggttttt gatttcctag
     7201 gtagggtatt taaacaaggt gcgttcaaac ccactcagta aaggtaaaaa ctccatccct
     7261 ccctcagggt ctcccaagga tcacacactt cctcaggcac agcaagtgac attatagtca
     7321 ctgcgtggtt tacttttcct cagtgtattt gctgtctaat gagtaagtat atgtcaacag
     7381 gttttcttct cctcttcata agaccctacc tatttgggga acaagagccy nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnngtttt ttgagaccgg gtttctctgt gtcgccctgg
     7501 ctgtcctgga actcactctg tagatcaggc tggcctcaaa ctcagaaatc cacctgcctc
     7561 tgcctcccaa gtgctgggat taaaggcgtg cgcctccact gcctggcata agagcccttc
     7621 ttaacagaca aaaagcaagt acaaaaaggg gaaaggaaca ataacaacaa caaaaagtaa
     7681 ggcaacagcc accaactctg accatctcca tccttcattc atcctggaga tcacacagta
     7741 cagggagaga accaggtctc ccatcttaat gtctatctgc ttaacacagc aattaaaaat
     7801 aatgctaaga gcccactgtc tgactaagaa ggtgatggaa gaatggtaga gagatgccag
     7861 gtctggggag gttgttatgg ccatagtgag tagcttaggt ttgatctgct gctgtgaatt
     7921 gtaacgctat atactggtgc tggttgcccc agggatgaga gaatcctcac agtacactaa
     7981 gtgatgttat gtaaccttgc ttaatttaaa actattggtt gaataaagat actgcagcct
     8041 atagctgggg ttggggggta agcaaatatg aaaacatgca tgagggctgg ccagatagag
     8101 ttaagagctg cccagatgga accttgcaag ctataagtca gggttatcgg tagggaaata
     8161 gacagtaatg gcttggatgt ctgcccagct ctagtgcttt aaaggcttat cataaatata
     8221 aaagttttgt ttcttttatc tgaggactga atgaacaaag gcggggtagg aaacccctta
     8281 tgagattaat aattcttaca tattctgtat ctaactgcag cctttgaaac atctttgata
     8341 aggatatgct gtctggtttc taagtagagt ttcttttagg aattaattgt aagttcttct
     8401 gctactgaat agagatcact ttcaaaatgt agaagctctg aacagaaaga atcattgtat
     8461 ctcgttcttc tatagtagca atccagtctt gcctgtctct gtctagcctt gtttccccct
     8521 cgggcagcag tatggcacag tcacttgcaa gcacatttgc tgaatgcagc tctaatttcc
     8581 ctggcatttt gtttggagag cttgtgcctt gttgttagca ggatagaaga ggccatgctc
     8641 atcttaaatt tagatgggtg ttatatataa tccaaactca gatctttata catgttccac
     8701 aagaattctt tcttcttccg caatcagaca tctctctagt ccagttttat tgctgctatg
     8761 gggtatgact gagtttggct gtagagtttg agctaactga aatgtcaaag tcattaaatc
     8821 accttgctta ggctcatact catgcctata tgtgcctggc tgtcttgagg ctgtaccatt
     8881 gtgcatgtcc caagaaggta cctccttgag tgttaccatg gcaacagaat gcagagccta
     8941 tgatatcttt ctaataacaa cagccaccca tcttaggagc tgttgttcta ttgctgttga
     9001 gagccactat gagcgtggca agtcttataa aagaaaacat ttagtggagg ctggcttaca
     9061 gttttaaaga cattatcatc ttggactgtt gctggaacag tagctaagag ttctacaccc
     9121 tggtctgtag gcagagagaa agagaaagag agagactgac tctggacttg ctgtgggctt
     9181 ttcaaacctc aaagcttgtc ccccaacaag gccacatctc ctaatccttt caaataatgc
     9241 gacttccccg ttgaccaagc attggaatct atgagcctgt tggcagccat tgtcattcca
     9301 actaccacac cattttaggg attggtgact aaatcaaggc agagaatgcc tgagggcagg
     9361 agttcctatt aactaagaag atatgttgcc agccccactt ttaatttaac aagatagact
     9421 aacagtgtta agtatgctga tatgttttaa aagccattaa tttattacaa tgatgatgct
     9481 tgtgtcccag tgcacacacg tgggtgtgcg cacacaacag gagcctatgg aggcattggc
     9541 ttgggtactt ccaacacctc cttgagacag gagacctcat tggccaagag cttgccaatg
     9601 gtctccagat gcattgcttt ctccacttgc aatgttggga ttacaggtgt gatgtctctc
     9661 ctggtgtttt acatgggttc tggggagttg aactcagatc ctcaaacatt tctgcagaag
     9721 atatcctagt ctatctaatg gcagaaatta tattttactt ttttccaatt gaattaaaat
     9781 aaaatttcat ggctggccag tggtggtagg cacctttaat cccagcactc aggagacaga
     9841 gccagagaat ctctaagttc aaggccagcc tggtctacag agcaagttag gatagccagg
     9901 gctgcacaga gaaagtctgt ttcaaaaaac ccaaaaccgt tggatagtgg tggcgcaagc
     9961 acttaatccc agcacttggg aggcagaggc aggtgaactc acacttctct gagttcgagg
    10021 ccagcctggt ctacagagtg agttccagga cagagtgagt tctgcacaga gaaaccctgt
    10081 ctttggaaaa acaaaacaaa caaaacaaaa caaaacaaaa caaaacaaaa caaaacaaaa
    10141 caaagaaagc cgactgagca agccagtaag taagcctcta gccctcttgg cctctgcttc
    10201 acttcctgcc tctggctgta tttcctgctc tgccttctct taatgatgga ctctaacctg
    10261 taagccaaag gaattcagtt ctcccaaagt tgttagtcat ggtctttatc agagtaagga
    10321 aatatataat accataataa ataattgtta ctgtcatgga acggaattaa tagttgacag
    10381 ataaaccttc aatgacttac acaaaaatgg atgccaatat gatcatggat acttagcagt
    10441 aaatgcttct gaaatggcca gataaaataa atccaagtat ggtggctggg ctgacacttg
    10501 taataccatc gctgaggtac aaagattgca gttgattctt ggttcaggtt gctttctttt
    10561 ttcttttttt ttttttaaga tttatttatt tgttatatgt aagtacactg tagctatctt
    10621 cagacacacc agaagaggga gtcagatctt gttagggatg gttgtgagcc accatgtggt
    10681 tgctgggatt tgaactcaga accttcggaa gagcagttgg gtgctcttac ctgctgagcc
    10741 atctcaccag cccctcaggt tgctttcaat gtcttgagga agacaagcaa aatataaact
    10801 caatcttttc tttttttgct acagaggcca ctacagcccg ggggggtcga cgcggccgcc
    10861 atggtcatag ctgtttgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10921 tttgtttatt tttctaaata cattcaaata tgtatccgct cacgagacaa taaccctgat
    10981 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    11041 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    11101 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    11161 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    11221 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    11281 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    11341 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11401 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11461 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11521 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11581 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11641 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11701 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11761 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11821 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11881 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11941 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    12001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    12061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    12121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    12181 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    12241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    12301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12421 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12661 tctggccttt tgcacggacc gtagagtcga gcagtgtggt tttcaagagg aagcaaaaag
    12721 cctctccacc caggcctgga atgtttccac ccaatgtcga gcagtgtggt tttgcaagag
    12781 gaagcaaaaa gcctctccac ccaggcctgg aatgtttcca cccaatgtcg agcaaacccc
    12841 gcccagcgtc ttgtcattgg cgaattggaa cacgcagatg cagtcggggc ggcgcggtcc
    12901 caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
    12961 cgacccgctt aacagcgtca acagcgtgcc gcacatcttg gtggcgtgaa actcccgcac
    13021 ctcttcggcc agcgccttgt agaagcgcgt atggcttcgt accccggcca tcagcacgcg
    13081 tctgcgttcg accaggctgc gcgttctcgc ggccatagca accgacgtac ggcgttgcgc
    13141 cctcgccggc agcaagaagc cacggaagtc cgcccggagc agaaaatgcc cacgctactg
    13201 cgggtttata tagacggtcc ccacgggatg gggaaaacca ccaccacgca actgctggtg
    13261 gccctgggtt cgcgcgacga tatcgtctac gtacccgagc cgatgactta ctggcgggtg
    13321 ctgggggctt ccgagacaat cgcgaacatc tacaccacac aacaccgcct tgaccagggt
    13381 gagatatcgg ccggggacgc ggcggtggta atgacaagcg cccagataac aatgggcatg
    13441 ccttatgccg tgaccgacgc cgttctggct cctcatatcg ggggggaggc tgggagctca
    13501 catgccccgc ccccggccct caccctcatc ttcgaccgcc atcccatcgc cgccctcctg
    13561 tgctacccgg ccgcgcgata ccttatgggc agcatgaccc cccaggccgt gctggcgttc
    13621 gtggccctca tcccgccgac cttgcccggc acaaacatcg tgttgggggc ccttccggag
    13681 gacagacaca tcgaccgcct ggccaaacgc cagcgccccg gcgagcggct tgacctggct
    13741 atgctggccg cgattcgccg cgtttacggg ctgcttgcca atacggtgcg gtatctgcag
    13801 ggcggcgggt cgtggcggga ggattgggga cagctttcgg ggacggccgt gccgccccag
    13861 ggtgccgagc cccagagcaa cgcgggccca cgaccccata tcggggacac gttatttacc
    13921 ctgtttcggg cccccgagtt gctggccccc aacggcgacc tgtacaacgt gtttgcctgg
    13981 gccttggacg tcttggccaa acgcctccgt cccatgcacg tctttatcct ggattacgac
    14041 caatcgcccg ccggctgccg ggacgccctg ctgcaactta cctccgggat gatccagacc
    14101 cacgtcacca ccccaggctc cataccgacg atctgcgacc tggcgcgcac gtttgcacgg
    14161 gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cctgcgctat
    14221 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
    14281 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
    14341 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
    14401 tcgcagccaa cgtcggggcg gcaggccctg ccatagccac gggccccgtg ggttagggac
    14461 ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg
    14521 gggtcagtcc acgactggac tgagcagaca gacccatggt ttttggatgg cctgggcatg
    14581 gaccgcatgt actggcgcga cacgaacacc gggcgtctgt ggctgccaaa cacccccgac
    14641 ccccaaaaac caccgcgcgg atttctggcg ccgccggacc aactaaacct gactacggac
    14701 cggtaaaacg acggccagtg ttaac
//
