LOCUS       Exported                3078 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  dsRNA expression .
ACCESSION   .
VERSION     .
KEYWORDS    U6-Lin28aIR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3078)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     5..22
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        122..362
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     122..142
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     293..312
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1119..1137)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1205..1309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1310..2170
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1528..1547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2341..2929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2830..2849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
       61 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcaat
      121 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      181 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      241 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
      301 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
      361 acgaaacacc aagcttggtt tgacacttgt ttcgcttccc gtctggcaag ggaaatatca
      421 cacagccttc tacaaaagaa cttccctagc ccagccactt ggcaaagcct cgggttctag
      481 ggtggttcct ccccacaaaa gccttcactt gctttcagat agagtgtggt aggagttcct
      541 ttgtctcatc ctttccttcc ctccaaggaa agacagggtg acactgggaa ccccggcctg
      601 agacacggca gtgcaagcta cccacgcaac ccacccttac ccccactttc tccactctgc
      661 agattgatgc ctctttcctt aaactcctgg atccagggag gaagaggaag agatccacag
      721 ccctgccctg ctcccagaag cccagaattg aggcccagga gtcagggtta ttctttggct
      781 aatggggagt ttaaggaaag aggcatcaat ctgcagagtg gagaaagtgg gggtaagggt
      841 gggttgcgtg ggtagcttgc actgccgtgt ctcaggccgg ggttcccagt gtcaccctgt
      901 ctttccttgg agggaaggaa aggatgagac aaaggaactc ctaccacact ctatctgaaa
      961 gcaagtgaag gcttttgtgg ggaggaacca ccctagaacc cgaggctttg ccaagtggct
     1021 gggctaggga agttcttttg tagaaggctg tgtgatattt cccttgccag acgggaagcg
     1081 aaacaagtgt caaaccaagc tttttttctg caggggcggc ctcgtgatac gcctattttt
     1141 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     1201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1321 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1381 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1441 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1501 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1561 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1621 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1681 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1741 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1801 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1861 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1921 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2041 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2101 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2161 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2521 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3001 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3061 ccgcagccga acgaccga
//
