LOCUS Exported 2674 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION ssRNA expression for dsRNA production. ACCESSION . VERSION . KEYWORDS U6-Lin28a-s SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2674) TITLE Petr Svoboda lab unpublished REFERENCE 2 (bases 1 to 2674) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2674 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 68..308 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 68..88 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 239..258 /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(661..679) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 747..851 /gene="bla" /label=AmpR promoter CDS 852..1712 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1070..1089) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1883..2471 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2372..2391 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2625..2642 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 61 agcaattgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 121 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 181 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 241 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 301 gaaaggacga aacaccgcgg ccgcggagtt taaggaaaga ggcatcaatc tgcagagtgg 361 agaaagtggg ggtaagggtg ggttgcgtgg gtagcttgca ctgccgtgtc tcaggccggg 421 gttcccagtg tcaccctgtc tttccttgga gggaaggaaa ggatgagaca aaggaactcc 481 taccacactc tatctgaaag caagtgaagg cttttgtggg gaggaaccac cctagaaccc 541 gaggctttgc caagtggctg ggctagggaa gttcttttgt agaaggctgt gtgatatttc 601 ccttgccaga cgggaagcga aacaagtgtc aaaccgcggc cgcttttttc tgcaggggcg 661 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 841 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 961 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 1021 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 1081 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 1141 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 1201 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 1261 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 1321 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 1381 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 1441 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 1501 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 1561 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 1621 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 1681 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1741 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1801 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1861 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1921 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1981 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 2041 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 2101 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 2161 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 2221 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 2281 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 2341 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 2401 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 2461 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 2521 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 2581 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 2641 aggaagcgga agagcgccca atacgcaaac cgcc //