LOCUS Exported 2674 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION ssRNA expression for dsRNA production. ACCESSION . VERSION . KEYWORDS U6Lin28a-as SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2674) TITLE Petr Svoboda lab unpublished REFERENCE 2 (bases 1 to 2674) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2674 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 80..320 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 80..100 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 251..270 /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(673..691) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 759..863 /gene="bla" /label=AmpR promoter CDS 864..1724 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1082..1101) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1895..2483 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2384..2403 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2637..2654 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 61 tcccgactgg aaagcaattg agggcctatt tcccatgatt ccttcatatt tgcatatacg 121 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt 181 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg 241 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt 301 atatatcttg tggaaaggac gaaacaccgc ggccgcggtt tgacacttgt ttcgcttccc 361 gtctggcaag ggaaatatca cacagccttc tacaaaagaa cttccctagc ccagccactt 421 ggcaaagcct cgggttctag ggtggttcct ccccacaaaa gccttcactt gctttcagat 481 agagtgtggt aggagttcct ttgtctcatc ctttccttcc ctccaaggaa agacagggtg 541 acactgggaa ccccggcctg agacacggca gtgcaagcta cccacgcaac ccacccttac 601 ccccactttc tccactctgc agattgatgc ctctttcctt aaactccgcg gccgcttttt 661 tctgcagggg cggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 721 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 781 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 841 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 901 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 961 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 1021 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 1081 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 1141 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 1201 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 1261 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 1321 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 1381 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 1441 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 1501 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 1561 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 1621 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 1681 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 1741 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 1801 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 1861 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 1921 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 1981 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 2041 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 2101 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 2161 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 2221 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 2281 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 2341 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 2401 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 2461 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 2521 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 2581 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 2641 agtcagtgag cgaggaagcg gaagagcgcc caat //