LOCUS Exported 3405 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level 0 part. Promoter. ACCESSION . VERSION . KEYWORDS pC0.049 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3405) AUTHORS Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick AJ TITLE CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria Based on the Plant MoClo Syntax. JOURNAL Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub 2019 Feb 28. PUBMED 30819783 REFERENCE 2 (bases 1 to 3405) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3405 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 78..172 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 328..357 /label=T3Te terminator /note="phage T3 early transcription terminator" promoter 369..403 /label=J23119 promoter /note="bacterial promoter (Registry of Standard Biological Parts BBa_J23119)" protein_bind 406..422 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 440..451 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" terminator 468..499 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" CDS complement(598..1413) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1321..1340 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 1405..1424 /label=pENTR-R /note="pENTR vectors, reverse primer" CDS complement(1771..2721) /codon_start=1 /gene="rep101" /product="RepA protein needed for replication with the pSC101 origin" /label=Rep101 /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" rep_origin 2769..2991 /label=pSC101 ori /note="low-copy replication origin that requires the Rep101 protein" ORIGIN 1 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta 61 ctgtccctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg 121 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag 181 ctcgtaaact tggtctgaca gctctagctc cggcaaaaaa acgggcaagg tgtcaccacc 241 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga cgtctaagaa 301 aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccggt 361 ctcaggagtt gacagctagc tcagtcctag gtataatgct agctattgtg agcggataac 421 aatttcacac atactagaga aagaggagaa atactaaatg tgagaccagt caaaagcctc 481 cgaccggagg cttttgacta aaacttccct tggggttatc attggggctc actcaaaggc 541 ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgct agtattatta 601 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc 661 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccaaag 721 aatggcaagg tcctggtaac ggtctgcgat tccgacccgt ccaacatcaa tacaacctat 781 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga 841 atccggtgag aatggcaaga gcttgtgcat ttctttccag acttgttcaa caggccagcc 901 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcatgc gtgattgcgc 961 ctgagcaaga cgaaatacac gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg 1021 taaccggcgc aggaacacgg ccagcgcatc aacaatattt tcacctgaat caggatattc 1081 ttctaatacc tggaaggctg ttttcccagg aatcgcggtg gtgagtaacc acgcatcatc 1141 aggagtacgg ataaaatgct tgatggtcgg gagaggcata aactccgtca gccagttgag 1201 acggaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa 1261 ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt gcccgacatt 1321 atcgcgagcc catttatacc catataaatc agcgtccatg ttggagttta agcgcggacg 1381 ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta 1441 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag 1501 attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca 1561 gctctagtag ttacattgtc gatctgttca tggtgaacag ctttgaatgc accaaaaact 1621 cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt 1681 tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct 1741 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt 1801 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt 1861 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa 1921 agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt 1981 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat 2041 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac 2101 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg 2161 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc 2221 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca 2281 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt 2341 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa 2401 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa 2461 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc 2521 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac 2581 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa 2641 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt 2701 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca 2761 attgagatgg gctagtcaat gataattaca tgtccttttc ctttgagttg tgggtatctg 2821 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc 2881 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa 2941 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta 3001 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag 3061 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat 3121 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg 3181 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga 3241 ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt 3301 ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct 3361 ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtca //