LOCUS Exported 6417 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Focal Adhesions, Excitation: 559, Emission: 600. ACCESSION . VERSION . KEYWORDS mRuby2-Zyxin-6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6417) AUTHORS Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ TITLE Improving FRET dynamic range with bright green and red fluorescent proteins. JOURNAL Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PUBMED 22961245 REFERENCE 2 (bases 1 to 6417) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6417 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 109..412 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 413..616 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 567..587 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 682..691 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 2412..2421 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2418..3128 /codon_start=1 /product="monomeric red fluorescent protein derived from mRuby, with improved photophysical properties (Lam et al., 2012)" /label=mRuby2 /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV AKFAGLGGGMDELYK" polyA_signal 3253..3374 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3290..3309) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3344..3363 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3381..3836) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3518..3539) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3730..3749 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3863..3967 /gene="bla" /label=AmpR promoter promoter 3969..4326 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3992..4012) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4177..4312 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4239..4258 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4361..5155 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4415..4434) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5025..5044 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5343..5362) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5387..5434 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5763..6351 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6252..6271 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata 61 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 121 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 181 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 241 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 301 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 361 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 421 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 481 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 541 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 601 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat 661 ctcgagctca agcttcgaat tccatggcgg ccccccgccc gtctcccgcg atccccgttt 721 cggtctcggc tccggctttt tacgccccgc agaagaagtt cggccctgtg gtggccccaa 781 agcccaaagt gaatcccttc cggcccaggg acagcgagcc tcccccggca cccggggccc 841 agcgcgcaca gatgggccgg gtgggcgata ttcccccgcc gcccccggaa gactttcccc 901 tgcctccacc tccccttgct ggggatggcg acgatgcaga gggtgctctg ggaggtgcct 961 tcccgccgcc ccctcccccg atcgaggaat catttccccc tgcgcctctg gaggaggaga 1021 tcttcccttc cccgccgcct cctccggagg aggagggagg gcctgaggcc cccataccgc 1081 ccccaccaca gcccagggag aaggtgagca gtattgattt ggagatcgac tctctgtcct 1141 cactgctgga tgacatgacc aagaatgatc ctttcaaagc ccgggtgtca tctggatatg 1201 tgcccccacc agtggccact ccattcagtt ccaagtccag taccaagcct gcagccgggg 1261 gcacagcacc cctgcctcct tggaagtccc cttccagctc ccagcctctg ccccaggttc 1321 cggctccggc tcagagccag acacagttcc atgttcagcc ccagccccag cccaagcctc 1381 aggtccaact ccatgtccag tcccagaccc agcctgtgtc tttggctaac acccagcccc 1441 gagggccccc agcctcatct ccggctccag cccctaagtt ttctccagtg actcctaagt 1501 ttactcctgt ggcttccaag ttcagtcctg gagccccagg tggatctggg tcacaaccaa 1561 atcaaaaatt ggggcacccc gaagctcttt ctgctggcac aggctcccct caacctccca 1621 gcttcaccta tgcccagcag agggagaagc cccgagtgca ggagaagcag caccccgtgc 1681 ccccaccggc tcagaaccaa aaccaggtgc gctcccctgg ggccccaggg cccctgactc 1741 tgaaggaggt ggaggagctg gagcagctga cccagcagct aatgcaggac atggagcatc 1801 ctcagaggca gaatgtggct gtcaacgaac tctgcggccg atgccatcaa cccctggccc 1861 gggcgcagcc agccgtccgc gctctagggc agctgttcca catcgcctgc ttcacctgcc 1921 accagtgtgc gcagcagctc cagggccagc agttctacag tctggagggg gcgccgtact 1981 gcgagggctg ttacactgac accctggaga agtgtaacac ctgcggggag cccatcactg 2041 accgcatgct gagggccacg ggcaaggcct atcacccgca ctgcttcacc tgtgtggtct 2101 gcgcccgccc cctggagggc acctccttca tcgtggacca ggccaaccgg ccccactgtg 2161 tccccgacta ccacaagcag tacgccccga ggtgctccgt ctgctctgag cccatcatgc 2221 ctgagcctgg ccgagatgag actgtgcgag tggtcgccct ggacaagaac ttccacatga 2281 agtgttacaa gtgtgaggac tgcgggaagc ccctgtcgat tgaggcagat gacaatggct 2341 gcttccccct ggacggtcac gtgctctgtc ggaagtgcca cactgctaga gcccagacgg 2401 atccaccggt cgccaccatg gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta 2461 tgaaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag 2521 aaggcaatcc gtacatggga actcaaacca tgaggatcaa agtcatcgag ggaggacccc 2581 tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt acttttatca 2641 agtacccgaa aggcattcct gatttcttta aacagtcctt tcctgagggt tttacttggg 2701 aaagagttac gagatacgaa gatggtggag tcgtcaccgt catgcaggac accagccttg 2761 aggatggctg tctcgtttac cacgtccaag tcagaggggt aaactttccc tccaatggtc 2821 ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag 2881 atggtggtct gaggggatac actcatatgg cactgaaagt tgatggtggt ggccatctgt 2941 cttgctcttt cgtaacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg 3001 gtatccatgc cgttgatcac cgcctggaaa ggttagagga aagtgacaat gaaatgttcg 3061 tagtacaacg cgaacacgca gttgccaagt tcgccgggct tggtggtggg atggacgagc 3121 tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt 3181 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 3241 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 3301 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 3361 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 3421 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 3481 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 3541 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 3601 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 3661 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3721 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3781 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg 3841 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 3901 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 3961 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag 4021 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 4081 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4141 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 4201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 4261 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 4321 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 4381 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 4441 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 4501 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 4561 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 4621 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 4681 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 4741 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 4801 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 4861 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 4921 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 4981 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 5041 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 5101 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 5161 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 5221 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 5281 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta 5341 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga 5401 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 5461 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc 5521 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 5581 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt 5641 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 5701 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 5761 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 5821 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 5881 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 5941 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 6001 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 6061 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 6121 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 6181 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 6241 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 6301 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 6361 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttcttt //