LOCUS Exported 10922 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.. ACCESSION . VERSION . KEYWORDS EF1a_FOXA1_P2A_Hygro_Barcode SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10922) AUTHORS Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P TITLE Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout. JOURNAL Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008. PUBMED 30448000 REFERENCE 2 (bases 1 to 10922) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10922 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(46..65) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 237..616 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 618..816 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 771..791 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 834..1014 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1061..1186 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1679..1912 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2097..2141 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2439..2556 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2692..3870 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2923..3861 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3818..3838 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" primer_bind 3887..3906 /label=T7 /note="T7 promoter, forward primer" promoter 3887..3905 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 5394..5450 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 5451..6476 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" protein_bind 6668..6701 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 6726..6906 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6932..6949) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6938..7162 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7208..7636 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7295..7314) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7505..7526 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7645..7665) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7650..7979 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7830..7965 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7892..7911 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8027..8074 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8093..8467 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8597..8718 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8634..8653) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8688..8707 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8767..8783) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8767..8783) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8780..8802) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8791..8807 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8815..8845) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8860..8881 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8998..9015) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9169..9757) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9249..9268) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9928..10788) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10551..10570 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10789..10893) /gene="bla" /label=AmpR promoter ORIGIN 1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc 841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg 1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat 1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg 1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa 1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct 1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac 1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca 1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa 1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga 1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag 1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag 1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga 1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc 1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc 1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt 1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta 1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta 2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga 2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa 2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag 2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg 2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt 2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa 2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga 2581 gatccagttt ggttaattaa tcgataagct ttgcaaagat ggataaagtt ttaaacagag 2641 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt 2701 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 2761 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 2821 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 2881 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt 2941 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc 3001 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg 3061 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc 3121 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag 3181 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt 3241 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc 3301 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca 3361 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg 3421 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga 3481 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc 3541 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc 3601 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg 3661 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc 3721 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg 3781 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa 3841 agtttttttc ttccatttca ggtgtcgtga ggaattagct tggtactaat acgactcact 3901 atagggagac ccaagctggc taggtaagct tgatatcgaa ttcctgcagc ccgggggatc 3961 ccgccaccat gttgggcacc gtgaagatgg aggggcatga gacaagcgac tggaattcct 4021 actacgcgga tacccaagaa gcgtattctt cagttcccgt aagcaatatg aactccggat 4081 tggggagcat gaatagtatg aacacgtata tgacaatgaa tacgatgacc accagcggca 4141 acatgacacc ggcctccttt aatatgtcat atgcgaaccc tggtcttggc gctggcctct 4201 caccaggtgc ggtcgctgga atgcccgggg ggagcgccgg agcgatgaac tccatgaccg 4261 ctgcgggcgt gacggccatg ggtacggccc tgtcacccag tggaatggga gctatggggg 4321 cccagcaagc cgcctcaatg aatggattgg ggccctatgc cgcggcgatg aatccctgca 4381 tgtcccctat ggcttatgcc cccagcaatt tgggtcgcag tagagccggc ggtggtggcg 4441 atgccaaaac cttcaagcga agttatcctc atgcgaagcc tccttattca tatatatcct 4501 tgattacgat ggcgatacag caggccccgt ctaagatgct gactctgagt gagatatacc 4561 agtggatcat ggaccttttt ccttactacc ggcaaaacca acagagatgg caaaactcaa 4621 tacgccatag cctttccttc aatgattgct ttgtcaaagt cgctcggagc cctgacaagc 4681 ccggtaaagg gtcctattgg acccttcatc cagatagcgg caatatgttc gagaatggtt 4741 gttatcttag acggcagaaa cgattcaaat gtgagaaaca gccaggtgcc ggcggtggtg 4801 gcggcagcgg ttcaggcgga agtggtgcca agggtgggcc tgagtctaga aaagacccca 4861 gcggagcaag caatccaagc gcggactctc ccctgcaccg cggtgttcat ggtaagacag 4921 gtcagcttga gggggcgcct gctccaggcc cggctgcgtc accgcaaaca ctggaccata 4981 gtggagctac agcgaccgga ggtgcttcag aactcaagac gcctgcgtcc tccactgcgc 5041 ctccgatctc cagtggtccc ggtgcacttg cctctgttcc tgcatctcat ccagcacacg 5101 gactcgcgcc gcacgagtcc cagctccatt tgaaagggga cccacactac agctttaacc 5161 acccattctc tattaacaat ttgatgtcat cctcagaaca gcagcataaa ctcgacttca 5221 aagcctatga acaggccctg cagtattctc catatggctc tacacttcct gcttctcttc 5281 cattggggtc tgcaagtgtg acaacgcgct ccccaatcga gccaagtgcc ctcgagcctg 5341 cttattatca aggagtatat tcccgaccag ttttgaatac aagtggatcc ggcgcaacaa 5401 acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg atgaaaaagc 5461 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg 5521 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc 5581 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt 5641 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca 5701 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc 5761 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg 5821 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat 5881 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa 5941 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt 6001 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg 6061 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg 6121 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc 6181 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg 6241 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg 6301 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg 6361 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag 6421 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataagttg 6481 gaactatttc ctggctgtta cgcggttttt tgaagcgccc cttactactg acgggcttgt 6541 taaacagtga accgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc 6601 catgcatcga acaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 6661 agatatcata acttcgtata gcatacatta tacgaagtta tcggctagct ggtccggact 6721 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 6781 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 6841 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 6901 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 6961 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 7021 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 7081 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 7141 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 7201 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 7261 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 7321 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 7381 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 7441 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 7501 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 7561 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 7621 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 7681 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 7741 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 7801 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 7861 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 7921 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 7981 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc 8041 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa 8101 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg 8161 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg 8221 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct 8281 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc 8341 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg 8401 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca 8461 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 8521 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 8581 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 8641 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 8701 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 8761 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 8821 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 8881 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 8941 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 9001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 9061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 9121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 9181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 9241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 9301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 9361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 9421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 9481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 9541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 9601 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 9661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 9721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 9781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 9841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 9901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 9961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 10021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 10081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 10141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 10201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 10261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 10321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 10381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 10441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 10501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 10561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 10621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 10681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 10741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 10801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 10861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 10921 cg //