LOCUS Exported 10904 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.. ACCESSION . VERSION . KEYWORDS EF1a_SOX10_P2A_Hygro_Barcode SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10904) AUTHORS Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P TITLE Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout. JOURNAL Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008. PUBMED 30448000 REFERENCE 2 (bases 1 to 10904) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10904 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2693..3871 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2924..3862 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3819..3839 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" primer_bind 3888..3907 /label=T7 /note="T7 promoter, forward primer" promoter 3888..3906 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3964..3973 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 5377..5433 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 5434..6459 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" protein_bind 6651..6684 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 6709..6889 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6915..6932) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6921..7145 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7191..7619 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7278..7297) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7488..7509 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7628..7648) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7633..7962 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7813..7948 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7875..7894 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8010..8057 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8076..8450 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8580..8701 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8617..8636) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8671..8690 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8750..8766) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8750..8766) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8763..8785) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8774..8790 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8798..8828) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8843..8864 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8981..8998) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9152..9740) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9232..9251) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9911..10771) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10534..10553 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10772..10876) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta atcgataagc tttgcaaaga tggataaagt tttaaacaga 2641 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 2701 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 2761 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 2821 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 2881 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 2941 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 3001 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 3061 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 3121 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 3181 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 3241 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 3301 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 3361 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 3421 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 3481 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 3541 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 3601 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 3661 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 3721 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 3781 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 3841 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 3901 tatagggaga cccaagctgg ctaggtaagc ttgatatcga attcctgcag cccgggggat 3961 cccgccacca tggcggagga gcaggaccta tcggaggtgg agctgagccc cgtgggctcg 4021 gaggagcccc gctgcctgtc cccggggagc gcgccctcgc tagggcccga cggcggcggc 4081 ggcggatcgg gcctgcgagc cagcccgggg ccaggcgagc tgggcaaggt caagaaggag 4141 cagcaggacg gcgaggcgga cgatgacaag ttccccgtgt gcatccgcga ggccgtcagc 4201 caggtgctca gcggctacga ctggacgctg gtgcccatgc ccgtgcgcgt caacggcgcc 4261 agcaaaagca agccgcacgt caagcggccc atgaacgcct tcatggtgtg ggctcaggca 4321 gcgcgcagga agctcgcgga ccagtacccg cacctgcaca acgctgagct cagcaagacg 4381 ctgggcaagc tctggaggct gctgaacgaa agtgacaagc gccccttcat cgaggaggct 4441 gagcggctcc gtatgcagca caagaaagac cacccggact acaagtacca gcccaggcgg 4501 cggaagaacg ggaaggccgc ccagggcgag gcggagtgcc ccggtgggga ggccgagcaa 4561 ggtgggaccg ccgccatcca ggcccactac aagagcgccc acttggacca ccggcaccca 4621 ggagagggct cccccatgtc agatgggaac cccgagcacc cctcaggcca gagccatggc 4681 ccacccaccc ctccaaccac cccgaagaca gagctgcagt cgggcaaggc agacccgaag 4741 cgggacgggc gctccatggg ggagggcggg aagcctcaca tcgacttcgg caacgtggac 4801 attggtgaga tcagccacga ggtaatgtcc aacatggaga cctttgatgt ggctgagttg 4861 gaccagtacc tgccgcccaa tgggcaccca ggccatgtga gcagctactc agcagccggc 4921 tatgggctgg gcagtgccct ggccgtggcc agtggacact ccgcctggat ctccaagcca 4981 ccaggcgtgg ctctgcccac ggtctcacca cctggtgtgg atgccaaagc ccaggtgaag 5041 acagagaccg cggggcccca ggggccccca cactacaccg accagccatc cacctcacag 5101 atcgcctaca cctccctcag cctgccccac tatggctcag ccttcccctc catctcccgc 5161 ccccagtttg actactctga ccatcagccc tcaggaccct attatggcca ctcgggccag 5221 gcctctggcc tctactcggc cttctcctat atggggccct cgcagcggcc cctctacacg 5281 gccatctctg accccagccc ctcagggccc cagtcccaca gccccacaca ctgggagcag 5341 ccagtatata cgacactgtc ccggcccgga tccggcgcaa caaacttctc tctgctgaaa 5401 caagccggag atgtcgaaga gaatcctgga ccgatgaaaa agcctgaact caccgcgacg 5461 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg 5521 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg 5581 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg 5641 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat 5701 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc 5761 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag 5821 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat 5881 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc 5941 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc 6001 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc 6061 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc 6121 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc 6181 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt 6241 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg 6301 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc 6361 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga 6421 aaccgacgcc ccagcactcg tccgagggca aaggaataag ttagaactat ttcctggctg 6481 ttacgcgaat caatcctcat atagcaacta ctgacgggct tgttaaacag tgaaccgccc 6541 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgaacaaaag 6601 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc ataacttcgt 6661 atagcataca ttatacgaag ttatcggcta gctggtccgg actgtactgg gtctctctgg 6721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 6781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 6841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta 6901 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 6961 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 7021 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 7081 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 7141 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 7201 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 7261 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 7321 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 7381 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 7441 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 7501 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 7561 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 7621 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 7681 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 7741 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 7801 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 7861 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag 7921 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt 7981 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg 8041 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc 8101 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 8161 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt 8221 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc 8281 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg 8341 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc 8401 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac 8461 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 8521 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 8581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 8641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 8701 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 8761 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 8821 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 8881 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 8941 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 9001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 9061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 9121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 9181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 9241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 9301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 9361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 9421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 9481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 9541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 9601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 9661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 9721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 9781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 9841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 9901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 9961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 10021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 10081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 10141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 10201 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 10261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 10321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 10381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 10441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 10501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 10561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 10621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 10681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 10741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 10801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 10861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac //