LOCUS       Exported               14994 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for 
            CRISPR genome editing in fission yeast.
ACCESSION   .
VERSION     .
KEYWORDS    pAH243
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Hayashi A, Tanaka K
  TITLE     Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing 
            in Fission Yeast.
  JOURNAL   G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 
            10.1534/g3.118.200976.
  PUBMED    30755408
REFERENCE   2  (bases 1 to 14994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1147..1212
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1219..1239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1264..5364
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5365..5412
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     rep_origin      6499..7280
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        8418..8774
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     misc_RNA        8881..8932
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(10069..10086)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10069..10085)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10078..10100)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10298..10753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10385..10404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10595..10616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10770..10789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10889..10911
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10949..10967)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11035..11139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11140..12000
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11358..11377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12171..12759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12660..12679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12913..12930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13047..13068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13083..13113
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13121..13137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13126..13148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13145..13161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13145..13161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(13610..14404)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
ORIGIN
        1 gaataagtca tcagcggttg tttcatttcc tatatttttt ttttattttt ttatttttta
       61 ataagggaaa atttaacgtc taaggataca gaagattgtt agcacattaa agtaataaag
      121 gcttaagtag taagtgcctt agcatgttat tgtatttcaa aggacataat ctaaaataat
      181 aacaatatca tttctcacaa gttattcaat tttctttttt ttttctaata atatcaagaa
      241 tgtattattt gtttgacata agtcaactaa tttatttaat atgctggatt aatcttgcag
      301 acatgtaaat taacaagttt tagtcaaata acgttgaagt ttcaatgaac tcaaataatt
      361 tctctttttt tttatataac cataatctga tttatatttt ccgcagggat caactgaagt
      421 tatgacattt ggattggatc acttataacc ttggtcgcca aataatacaa aaatcagcgt
      481 tataaaacaa agaaggtttt tgttaagaaa ttaatcctct ttcttgataa gaaagttgaa
      541 ccgaaattgc agatactgat atatgaaaat aatacccaca attttgggaa tagcgcaagc
      601 ctcaatttaa acaataggtg aggacacatg ataatgacct caatgattgt tagaagaaaa
      661 gagcctcatt acaaaatcga aaaatgaatg gttgggtaca agtttccaaa acatggtaaa
      721 gtggactttg cgtatgagac gtaaatagaa aaaaacactt gttatatgtt ttctagaatt
      781 attgttgtct ctttatggtt ggatgatgca aaatagtaat ttcggttagt tgctgtaaaa
      841 caccacgaga caaatagata tggatattta ttaaatcagg aaaaacgtaa ctctcggcta
      901 ctggatggtt cagtcaccca acgattactg gggagagaaa acagggcaaa agcaaagctt
      961 aaaggaatcc gattgtcatt cggcaatgtg cagcgaaact aaaaaccgga taatggacct
     1021 gttaatcgaa acattgaaga taaaggaaga ggaatcctgg catatcatca attgaataag
     1081 ttgaattaat tatttcaatc tcattctcac tttctgactt atagtcgctt tgttaaatct
     1141 cgaatggact ataaggacca cgacggagac tacaaggatc atgatattga ttacaaagac
     1201 gatgacgata agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca
     1261 gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
     1321 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
     1381 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
     1441 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
     1501 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     1561 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     1621 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     1681 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     1741 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     1801 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     1861 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     1921 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     1981 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     2041 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     2101 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     2161 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     2221 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     2281 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     2341 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     2401 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     2461 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     2521 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     2581 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     2641 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     2701 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     2761 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     2821 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     2881 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     2941 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     3001 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     3061 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     3121 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     3181 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     3241 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     3301 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     3361 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     3421 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     3481 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     3541 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     3601 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     3661 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     3721 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
     3781 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     3841 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     3901 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     3961 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     4021 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     4081 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     4141 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     4201 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     4261 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     4321 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     4381 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     4441 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     4501 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     4561 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     4621 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     4681 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     4741 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     4801 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     4861 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     4921 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     4981 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     5041 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     5101 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     5161 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     5221 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     5281 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     5341 gacctgtctc agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca
     5401 aaaaagaaaa agtaagatct ccccgggtaa aaggaatgtc tcccttgcca gtactgctag
     5461 ggtttttctt tcaaactatg gaagcccatt caagctgcat attacgattt tgtttttcgc
     5521 ttttagaaag tggtttagat gagataatag aaaaattctt gatctccgac aacgagtact
     5581 tttatttttt ttgctaatca ctttactcaa tattagctcg aaatcgtaga aacgtagacg
     5641 ggtgcgggat accgagtggt gtagttaaga atttttataa accacgtggc ccaaaaatat
     5701 gaacccaaaa cgtttataca tgagtatact ttaagaaggc tatacccctt cgtgttagat
     5761 gtagttttag ctacccaacc cgagtctatg agcttgactt cagatgtaga aggcattaaa
     5821 tcgttttgaa tattaattaa aaaacgatga aaattaaata tttaaaagca atcatacgct
     5881 gaaaatttag tgctgtggct aatccttcaa catggaaatg ccataaaagt gactttgaca
     5941 aaaaaaaaag tatatacagg tagtaaactc atctacttca ttgactttgt ttacagcatg
     6001 tggaaggagg aatatttatt gctaaatcgt agtttaacat tcaataagta atactattga
     6061 aattcgacaa gattggccgc atggatgaaa aagaggcatt ttgctttggg agaattagtc
     6121 caaattagaa ctgaaaaaaa aaactttacg aggcaaaaat gtcggattga gatcgtaaaa
     6181 gttcgctcgt cgtcttttgc tttgtgattg ttttcatgga tacatcttgc tggatattta
     6241 aattttagta ctatgtataa gatattctat aaatgtttta tcacccaaac ctgttagcgc
     6301 cttcttaatt ctattcaatc tggcttttgc tctgagacta cttcttggac tttcactact
     6361 tgttagttat acggaatttg tgtaattaga agtgaaataa tcctttctat tagtaatgcg
     6421 agctcgaatt cgagtctaac tccttaacca ctcggacata gtgacttatc tgacactcat
     6481 tgtaaattaa tatatatata ggcattttgt ttagttaaag gtacttaagt aattagtata
     6541 aacgaaccaa ttttataatc aggaagttaa gtgaatggta gcacatgtcg taaaaattgt
     6601 gaatttttat tgaataatat tttaaataca agcctttcta gactaggtat actcataaac
     6661 atatatgagc aaaaggatag aggagattac attgcatctt ctacaaattt atttattgcc
     6721 ctttactgaa aaattaaata atgagtacta aatgataaaa agcgctcagt acaagaaaga
     6781 ttgcaaaaat attgcattct tcatgaatta aagttgcata taaggcatat tgaaagtaat
     6841 agtactaaaa cagcagttag cgaaaattaa tagaattata ttcgcaagac aattgtgaca
     6901 ttaaattaaa aaattgtaga atttttacta tcctctttaa cgccatgagc ctttataaaa
     6961 aggttaaatt agttttaaca ttcttttttt gagtaagagt ttacaattta tcaaaacctg
     7021 tgttattata ttcattagtt tcaatttatt agcatctaga gaaaaatcaa ttggcagtta
     7081 cattgttgga atttatgaag aaaaagaatc tacaacggag aataagttgc tgatctttcc
     7141 ctaaaattgt atattttgct gagcttattt tgacatttcg ttgaagtttt ctctaattcg
     7201 cattcatttt aagtaaaaca atgagaaata aaattacaaa aaatacaaat taaaatacaa
     7261 tttttagcta taattataga cgatgccctt gtatcccatt ctgtctcgct tgcccctact
     7321 ttttatcttt tatataccat aatgaacgct gccgctacta accatacccc gattttacat
     7381 ttcggactcc caaggacgta caaaatagaa aactatagaa aaaaataatc agaaaatagc
     7441 atgtcatctc tttgtaaaac gcggggaaat cataacatag tataatagga atcatatgac
     7501 atttactctt gcatagcaat atcctcttct tcgccatcag caatccgacg agcatatgct
     7561 tcagcctctt gcaaaagctc atcaggaacg ggtgtatgaa taccgtttgt ttcaacacca
     7621 acccaagtca tctcaatttc atgctctttg ttgttttctt cgtcatgagt tgcgtataat
     7681 ctaaaaaaga gtgaattatt agcaaataga atgaaaaaaa attgtactca acaaagcaaa
     7741 acgtacattc tagcagcttc tttaaccgca tctttcattt ttaatgagga aaaattcagt
     7801 ttttctaatt cgtttcgagc tacttgccga cctttaccag ctgcagcacc gttgtaaccc
     7861 caataaacac cattaggttc aaccatatac aaatgtggac cttctgagtc ataagtagca
     7921 acaaaagaca taacaccgaa tggacggacg ctagaatagc aagtgaaaag ttgaacgtag
     7981 ttccccaagc gatcagcaat aactgttcca gggatgggag atccgtagtt atctctccat
     8041 gaggtggctt cgtcccttgc acgttttacg atgtgttggc catcaggaat aaagccagta
     8101 gtagcaatac caatgtgtcg atcgaccgac ccaattctgt tatttactcg cggcttaagt
     8161 agtttgctag taactacctt ttctagagct agaataactc catcttcaca ttttattcca
     8221 atgcaagtac taaatacaat gttaaaatca atcgataagg aaattcaatc caccttacct
     8281 tgcattttcg acggctttat acgcgtactc cgcttggaat aaacgaccat ccggggagaa
     8341 gaataatcct aaatcgtatc cagtaccaat tgaactctgt ttgttaatgt gatgtgctac
     8401 tccttgctaa cttaccattt gcttatgttg gtggtagttg gcatgcgtag actgatgact
     8461 agtcagcaag gagcgtagaa cagtcacact cgttatatat gtgcttccaa gaaaactcaa
     8521 gaatttacca ttagcaaaca cttttttgaa atgttagaca tttaaatgac gaaggcatat
     8581 agaagctttg aataggtgtt gtaaagtgtt gatttatgtg acgctgaggg tgcgcatgaa
     8641 aggaatgttg ggtcacgatt attaaacagt ttgctagctt ggacacttga gtattggaag
     8701 ttgttgaatt ctaaaaaact ttcagttgat ttgaatagtt gctgttgcca aaaaacataa
     8761 cctgtaccaa agaacaccgg gtcttcgaga agacctgttt tagagctaga aatagcaagt
     8821 taaaataagg ctagtccgtt atcaacttga aaaaagtgag tggcaccgag tcggtggtgc
     8881 cctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca ggtacggtta
     8941 tttgttagca atggtaggag aaaattaggg attgtttacc aaaacaacgt gaacaaatag
     9001 ttatagtttt aatttaactc atgaacatat tatcggaaaa aatctactca tttatgtctg
     9061 ggtttagctc cctttatggt ttcgttttgc agtagaatac actatggtat ttattttgtg
     9121 cactacaaac cgtgcttttt atgtgaacgt tttgatgaaa acgttctaga gattgattta
     9181 ttattcaatg ttaaagtaac tcattttgct atatgtatct ttttttcagc gaactttcgt
     9241 tactgagcag ttcaagcagt gatttggcat tatggtatca ataaagacat atcattaagg
     9301 caaggctacg gaaagaaaaa agaaggtttt taaattaata tacaacaaat taaccgtgat
     9361 cggacagtat atttgatgca aagatataat gaaagttggg aaaataagaa tatagcactc
     9421 aaatgattgc cttacaaaag cattgctttc tctacaggta aggttgaagt gcctttacgg
     9481 tatcttcaaa aaccgcatcg ggatcttgct cggcatcgat ggttatcaga cgattttgtg
     9541 atttcagata ttcgacgaca ggcatgctgg ctttagtata tgtaacaaag cgtttcttga
     9601 tcgactcaat gttgtcatca gaacggccac tagtcttacc acgatgaata agacgcttca
     9661 acatggtttc ctgaccacaa cggaaataaa gagcaaactt agcggggcag accgactttt
     9721 caaaaccttc gcattgatcc atctcacggg ggaaaccgtc aatcaagaac ttgtcaattc
     9781 ctttatcatg gcattccttc attttagtct ctaaaaggga tatagttatt tccatgggca
     9841 caattttacc atctttgata tattctttaa taaggtttcc gtatttgctg ccaggacgcg
     9901 cgtttgcaag aagaaaggaa acaatggaga agagatcatc catttgtatg taaattttag
     9961 taaacttgaa gaaatcacta acaacttctc ttacttaggg attctatgca aaaccttgta
    10021 aatcatctga tggaggactc gatttaatga attggaaggt tggaattcac tggccgtcgt
    10081 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    10141 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    10201 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
    10261 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
    10321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
    10381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10501 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    10621 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
    10681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    10741 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    10801 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
    10861 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
    10921 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
    10981 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
    11041 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    11101 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    11161 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    11221 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    11281 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    11341 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    11401 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    11461 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    11521 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    11581 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    11641 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    11701 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    11761 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    11821 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    11881 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    11941 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    12001 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    12061 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    12121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    12181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    12241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    12301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    12361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    12781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    12841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    12901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    12961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
    13021 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    13081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    13141 cacacaggaa acagctatga ccatgattac gccaagcttg tgatattgac gaaacttttt
    13201 gacatctaat ttattctgtt ccaacaccaa tgtttataac caagttttat cttgtttgtc
    13261 tacatggtat tttacattca tctacataca tctttcattg gctttgtaca tagttatcat
    13321 tacaagtcta aaaaaattca ctcttttctt attcaatgtc aatccaagag aaaagattgt
    13381 ggtaatgttg taggagcatg tttaataaat tactatagca aattactttt tattcccaag
    13441 gtgtttatct ataatagtta atattttagt cgctacataa aattttacca aagagtactt
    13501 gtatactaat tctaaatgcc ttctgacata aaacgcctag gaaaacaaac gcaaacaagg
    13561 catcgacttt ttcaataacc aaccaaaaaa attttacatt agtctttttt taatgctgag
    13621 aaagtctttg ctgatatgcc ttccaaccag cttctctata tctcttggct tcgacaacag
    13681 gattacgacc agctccatag actccacgac caacaatgat gatatcgcta ccgcagttta
    13741 caatcacttc ttcaggagta cgatattgct gtcccagccc gtctccttta acatccaagc
    13801 cgataccagg ggacatagtt atgtagtcgc tttgaaggtt aggaaatcga cgaccagcta
    13861 taaagccaaa gcaaaaatcg gtatgcttct caaaccattc taaggttttc tctgtgtagg
    13921 aaccagtagc caaagagcct ttggatgaca tttcagccaa aagcaagaga ccacgtccca
    13981 aaggtaaacc aacttctttg aggccttgta taataccctc gcctggcact gtatggcaat
    14041 ttgtgatatg agcccaagaa gcaattttgt acacaccaga tgcatattgt agcttgacgg
    14101 tatttccaat gtctgcgaat ttgcgatcct caaagataag aaaacgatgc tttttaccta
    14161 aggccaccag tttttctacc atatcctggt cgaaatcctc gacaacgtca atatgtgtct
    14221 tgataacaca gacatagggt ccaattttat ctaccaattc taagatttcg gatttcttcg
    14281 tcaaatcgac cgcgactgac aagttgcttt gcttttcttc catcaaagcc aacaattcct
    14341 tggcaatggg atttttcatc ccctcagctc tagctgaata gctttgaaat actctagcat
    14401 ccataacttt gcttttaaac ctttaatttc gatccaagca aaaaagaggt tcttggtagg
    14461 acaatacggt aagaaaacac gacatgtgca gagatgccga cgaagcatag ttaaactggg
    14521 atggtaaaat caattaagaa tttataaaga caaaattgta taagtctcta aaacatctta
    14581 attatacctc acagaactat ctaaaatata ttcacaaagt gcaaacatta tcatgaaaaa
    14641 gaaccatttt aatttaaagc aagggcatta aggcttattt acagaatttc ttacttttgt
    14701 aaagattata aggctgatta tctttttcac catgccaaaa attacacaag atagaatgga
    14761 tgtttgaaat taaacgtgag tatacaaaca aatacactag gtaaatcgaa acattttttt
    14821 ctccattaag taacaaattc ctatttagag aaagaatgct gagtagatta aataatctat
    14881 acaaactttt ttaacacaaa tgcatacata tagccagtgg gatttgtagc taagcttgca
    14941 tgcctgcagg tcgatcgact ctagaggatc agaaaattat cgccataaaa gaca
//
