LOCUS Exported 1950 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION LexA UAS. ACCESSION . VERSION . KEYWORDS pWS480 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1950) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 1950) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1950 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 317..351 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(422..1081) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 996..1015 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1263..1851 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1752..1771 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc aaacgggtag tccatcgttg taggatactg tatatacata cagtagtcac 61 gtcgtttact gtatatactc acagtacgct attgcaacta ctgtatatac acccagtaga 121 gtaggtgact actgtatgag catacagtag tgacaaccac ttactgtata taaatacagt 181 agtggtcatc gatactgtat ataaaaccag taggcaatcg tttgtactgt atgtacatac 241 agtatacctc gcaactactg tatataaaca cagtaattaa cttgtaatat tctaatcaat 301 tgatgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag 361 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa 421 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga 481 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt 541 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg 601 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat 661 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg 721 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg 781 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct 841 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt 901 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa 961 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat 1021 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca 1081 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc 1141 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc 1201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 1261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 1321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 1381 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 1441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 1501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 1561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 1621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 1681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 1741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 1801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 1861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 1921 gcgttatccc ctgattctgt ggataaccgt //