LOCUS       Exported                1813 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pWS497
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      180..214
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(285..944)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     859..878
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1126..1714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1615..1634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attgatataa aagagaagca aataactcct tgtcttgtat caattgcatt
       61 ataatatctt cttgttagtg caatatcata tagaagtcat cgaaatagat attaagaaaa
      121 acaaactgta caatcaatca atcaatcatc acataaaaga tctatgtgag accagaccaa
      181 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      241 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      301 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      361 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      421 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      481 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
      541 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      601 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      661 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      721 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      781 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      841 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      901 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
      961 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1021 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1081 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1141 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1201 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1261 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1321 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1381 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1441 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1501 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1561 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1621 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1681 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1741 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1801 tgtggataac cgt
//
