LOCUS Exported 2709 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION P-factor GPCR. ACCESSION . VERSION . KEYWORDS pWS626 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2709) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 2709) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2709 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1076..1110 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(1181..1840) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1755..1774 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 2022..2610 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2511..2530 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc atatgaggca accttggtgg aaagacttta ctattcccga tgcaagcgca 61 attattcacc aaaatattac cattgtgtct attgtcggag agattgaagt gcctgtttca 121 acaattgatg catatgaaag ggataggtta ttaactggaa tgactttatc tgcccaatta 181 gctttaggag tgttaaccat tttaatggtt tgtctgttat catcaagcga aaaacgtaaa 241 caccctgttt ttgtttttaa ttcggcaagt attgttgcaa tgtgtttacg cgccatttta 301 aatatcgtga ccatctgctc gaattcgtac agtatcctcg ttaattacgg gtttatctta 361 aacatggttc atatgtatgt gcatgtgttt aatattttaa ttttattatt agcaccggtg 421 atcattttta ctgctgagat gtcgatgatg attcaagtta ggatcatttg tgcacatgat 481 aggaaaacac aacgtatcat gactgttatt agtgcctgct taactgtttt agttctcgca 541 ttttggatta ctaacatgtg tcaacagatt cagtatctct tatggttaac tcctttatcg 601 tcgaaaacca ttgttggata ctcttggccc tactttattg ctaaaatctt atttgctttt 661 tcgattattt ttcacagtgg tgttttttca tacaaactct ttagggccat cttaatccgc 721 aaaaaaattg ggcaatttcc ttttggtccg atgcagtgta ttttagttat ttcgtgccaa 781 tgtttaattg ttcctgctac ctttactatc atcgatagtt ttatccatac gtatgatggc 841 ttttcgtcta tgactcaatg tctcctgatc atttctttac cattatcgag tttatgggcg 901 tctagtacag ctctcaaatt acaatcgatg aaaacttcat ctgcgcaagg agaaaccacc 961 gaggtttcga ttagggttga taggacgttt gatatcaaac atactcccag tgacgattat 1021 tcgatttctg atgaatctga aactaaaaag tggacgggat cctgagacca gaccaataaa 1081 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac 1141 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact 1201 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg 1261 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 1321 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg 1381 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga 1441 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 1501 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 1561 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 1621 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 1681 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt 1741 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 1801 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg 1861 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt 1921 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc 1981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2041 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2101 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2161 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2221 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 2281 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 2341 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 2401 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 2461 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 2521 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 2581 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 2641 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 2701 gataaccgt //