LOCUS Exported 2733 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION PbPeptide GPCR. ACCESSION . VERSION . KEYWORDS pWS2119 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2733) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 2733) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2733 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1100..1134 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(1205..1864) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1779..1798 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 2046..2634 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2535..2554 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc atatggcacc ctcattcgac cccttcaacc aaagcgtggt gttccacaag 61 gccgacggaa ctccattcaa cgtgtcaatc catgaactag acgacttcgt gcagtacaac 121 accaaagtct gcatcaacta ctcttcccag ctcggagcat ctgtcattgc aggactcatg 181 cttgccatgc tgacacactc agaaaagcgt cgtctgccag ttttcttcct aaacacattc 241 gcactggcca tgaactttgc ccgcctgctc tgcatgacca tctacttcac cacgggcttc 301 aacaagtcct atgcctactt tggtcaggat tactcccagg tgcctgggag cgcctacgca 361 gcctctgtct tgggcgttgt gttcaccact ctcctggtaa tcagcatgga aatgtccctc 421 ctgatccaaa caagggttgt ctgcacgacc cttccggata tccaacgtta tctactcatg 481 gcagtttcct ccgcgatttc cctgatggcc atcgggttcc gccttggctt aatggttgag 541 aactgcattg ccattgtgca ggcgtcgaat ttcgcccctt ttatctggct tcaaagcgcc 601 tcgaacatca ccattacgat cagcacatgt ttcttcagtg ccgtctttgt tacgaaattg 661 gcatatgcac tcgtcactcg tatacgacta ggcttgacga ggtttggtgc tatgcaggtt 721 atgttcatca tgtcctgcca gactatggtg attccagcca tcttctcaat tctccaatac 781 ccactcccca agtacgaaat gaactccaac ctctttacgc tggtggccat tttcctccct 841 ctttcctcgc tatgggcttc agttgctacg agatccagtt tcgagacatc ttcttccggc 901 cgccatcagt atctttggcc aagcgaacag agcaataacg tcaccaattc ggaaattaag 961 tatcaggtca gcttctctca gaaccacact acgttgcggt ctggagggtc tgtggccacg 1021 acactctccc cggaccggct cgacccggtt tattgtgaag ttgaagctgg cacaaaggcc 1081 ggatcctgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc 1141 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat 1201 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc 1261 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct 1321 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat 1381 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca 1441 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt 1501 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa 1561 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca 1621 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa 1681 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg 1741 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa 1801 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct 1861 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg 1921 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa 1981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 2041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 2221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2701 cctgcgttat cccctgattc tgtggataac cgt //