LOCUS       Exported                3729 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Pheromone responsive transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    pWS939
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      2096..2130
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2201..2860)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2775..2794
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3042..3630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3531..3550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgaaagt ccaaataacc aatagtagaa cagaggaaat cttaaaagtt
       61 caagctaata atgaaaacga tgaagtcagt aaagctactc cgggcgaagt tgaagaatcg
      121 ctaaggttaa tcggcgatct aaaattcttt ttagccacag cgccggtaaa ttggcaagaa
      181 aaccaaatta taaggcgata ctatctgaat agtggacaag gctttgtctc ttgtgtattt
      241 tggaacaatc tatactatat tacaggtact gatattgtca aatgttgtct ttacagaatg
      301 caaaagtttg ggagagaagt agttcaaaag aaaaaatttg aagagggtat tttttcagat
      361 ttaagaaatc tcaaatgtgg tatagatgca actttagaac aaccaaagtc cgaatttttg
      421 tcgtttctat tcagaaatat gtgtctgaaa acccaaaaaa agcagaaagt atttttttgg
      481 ttcagtgtag cacatgataa gttgtttgcg gatgcgttgg aaagagattt aaaaagagaa
      541 agtttgaatc agccttcaac gactaagccc gttaatgagc ccgccttatc tttttcatat
      601 gattcctcat ctgataagcc tctctacgat cagttacttc aacatttaga ttctagaagg
      661 ccatctagta caacaaaatc agataattcg cctccaaaat tagaaagcga gaattttaag
      721 gataatgagt tggtaacagt aactaatcag ccgcttttag gcgttggcct catggatgac
      781 gatgcgccag aatccccctc tcaaattaat gattttattc ctcagaaatt gattatagaa
      841 cccaatactc tcgaattgaa tggtctaaca gaagaaacgc ctcatgactt acccaagaat
      901 accgctaagg gcagagatga agaagatttt cctctcgact attttcctgt atctgttgaa
      961 taccctacgg aggaaaatgc gtttgatccg ttccctccac aggcttttac gccagctgcc
     1021 ccttccatgc ctatttccta tgataacgtg aatgaaaggg attctatgcc cgttaattct
     1081 cttcttaata gataccccta tcagttatca gtggcaccca ctttcccagt gccaccatca
     1141 tcatcgaggc aacattttat gacaaatcgg gatttttatt catctaacaa taacaaggaa
     1201 aaattggtat ctcctagcga ccctaccagc tacatgaagt atgacgaacc agttatggat
     1261 tttgatgaat ctcggccaaa tgaaaactgt acaaatgcaa aatctcacaa ctctggccag
     1321 caaactaaac aacaccaatt atattctaac aacttccagc aatcttaccc aaacggaatg
     1381 gttccaggat actacccaaa aatgccgtat aatcccatgg ggggggatcc tctactcgat
     1441 caagcctttt atggcgcgga cgattttttc tttccaccag aaggatgtga taacaatatg
     1501 ctgtatccac aaactgcaac ttcatggaat gttttgcccc ctcaagctat gcaaccagct
     1561 ccaacctatg ttgggaggcc atacacaccg aattatagat cgacaccagg ttccgcgatg
     1621 ttcccataca tgcaaagttc aaattccatg cagtggaaca ctgctgtttc accttatagt
     1681 tcgagagcac catctacaac tgctaaaaac tatcctccta gcacatttta ttctcaaaat
     1741 ataaatcaat acccacggcg aagaactgtg ggaatgaagt catcacaagg aaatgttcca
     1801 acaggtaata aacaatctgt gggcaagtct gcaaaaattt caaagcctct acatattaag
     1861 acaagtgctt atcagaagca atacaaaatc aacttggaaa cgaaagccag gccaagtgct
     1921 ggtgacgaag attctgctca tcctgataag aacaaagaaa tttcgatgcc tactccggat
     1981 tccaatactt tggtggtcca gtcagaagaa ggtggagctc attcacttga ggtagatacc
     2041 aatcgaaggt ccgataaaaa ccttccagat gcaaccggat cctgagacca gaccaataaa
     2101 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     2161 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     2221 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     2281 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     2341 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2401 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     2461 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2521 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2581 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2641 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2701 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2761 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2821 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2881 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     2941 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
     3001 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3061 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3661 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3721 gataaccgt
//
