LOCUS Exported 2313 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION LexA bacterial repressor. ACCESSION . VERSION . KEYWORDS pWS926 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2313) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 2313) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2313 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 634..654 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 680..714 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(785..1444) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1359..1378 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1626..2214 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2115..2134 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc atatgaaggc tttaactgca agacaacagg aagtttttga tttgataaga 61 gatcatatat ctcaaactgg aatgccacca acaagagctg aaatagctca aagattagga 121 tttagatctc caaacgctgc agaagaacat ttgaaagctt tggcaaggaa aggtgtcatc 181 gaaattgtct ctggtgcttc aagaggtatt agactattac aagaagagga agaaggattg 241 ccattggttg gtagagttgc tgctggtgaa ccattattag cacagcaaca tattgagggt 301 cattatcaag tagatccatc tttattcaaa ccaaatgctg attttttgtt gagagtaagt 361 ggtatgtcta tgaaagatat tggtatcatg gatggtgact tattggctgt tcataaaact 421 caagatgtta gaaacggtca agttgtcgtt gccagaattg atgatgaagt cactgttaaa 481 agattgaaga aacaaggtaa taaagtggaa ttgttaccag aaaattctga atttaagcca 541 attgttgttg atttaaggca acaatctttt actattgaag gtttagctgt cggtgttatc 601 agaaatggtg attggttagg ttcaggttct cctccaaaaa aaaagagaaa agttggatct 661 ggttcttgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc 721 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat 781 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc 841 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct 901 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat 961 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca 1021 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt 1081 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa 1141 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca 1201 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa 1261 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg 1321 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa 1381 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct 1441 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg 1501 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa 1561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 1801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 1921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 1981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2281 cctgcgttat cccctgattc tgtggataac cgt //