LOCUS       Exported                2850 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Beta-estradiol inducible DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    pWS930
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1217..1251
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1322..1981)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1896..1915
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2163..2751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2652..2671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgaggcc ctacgcttgc cctgtagaaa gttgtgatag gagattcagc
       61 agatctgacg agctgactag gcatattcgt attcacaccg gccaaaagcc tttccaatgt
      121 agaatctgta tgagaaactt cagcagaagt gaccatttga ctacgcacat tagaacccac
      181 accggcgaga agccgttcgc gtgtgacatt tgtggaagaa agttcgccag atctgatgaa
      241 agaaagagac atacaaagat ccatacagga ggtgggggca ctccggcagc agcctcaacc
      301 ctagaagatc caagcgctgg agacatgagg gcagccaact tgtggccaag cccattaatg
      361 atcaaaagat ctaagaagaa ttctcttgca ctgtctttga ccgctgatca aatggtttcc
      421 gcactacttg atgctgaacc acctatattg tacagtgaat acgacccaac tcgtcctttt
      481 tccgaggctt ccatgatggg tcttttgaca aatctagcag acagggaatt agttcatatg
      541 attaattggg ccaaaagagt tcccggcttt gttgacttga cattacatga ccaggtacat
      601 cttcttgaat gcgcgtggtt agaaatattg atgatcggat tagtgtggag aagtatggag
      661 caccctgtaa aattattatt tgcacctaat ttattattgg acaggaacca aggtaaatgc
      721 gtagaaggta tggttgagat ttttgatatg cttcttgcca ctagctccag gttcagaatg
      781 atgaatttgc aaggagaaga attcgtttgc ctgaaatcaa ttattttgtt aaatagcggt
      841 gtctatacat ttttaagctc aactctgaaa agcctggaag agaaagacca tatacataga
      901 gtgttggaca agatcactga tacattgatt caccttatgg cgaaagcagg attgactctg
      961 cagcaacagc atcagaggct agctcagtta ctactaattt tgtcacacat tagacatatg
     1021 tctaacaagg gtatggagca cctgtattct atgaagtgta aaaacgtcgt tcctctgtat
     1081 gatttattac tagaaatgct ggacgctcac aggttgcatg caccaacttc tagaggcgga
     1141 gcctccgttg aagaaaccga ccaatcccac ctagcgacag cagggtctac atctagcggt
     1201 tcttgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1261 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1321 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1381 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1441 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1501 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat
     1561 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1621 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1681 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1741 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1801 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1861 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1921 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1981 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2041 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
     2101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2281 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2821 gcgttatccc ctgattctgt ggataaccgt
//
