LOCUS       Exported                3087 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Pheromone responsive domain.
ACCESSION   .
VERSION     .
KEYWORDS    pWS932
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1454..1488
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1559..2218)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2133..2152
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2400..2988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2889..2908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attctagacc atctagtaca acaaaatcag ataattcgcc tccaaaatta
       61 gaaagcgaga attttaagga taatgagttg gtaacagtaa ctaatcagcc gcttttaggc
      121 gttggcctca tggatgacga tgcgccagaa tccccctctc aaattaatga ttttattcct
      181 cagaaattga ttatagaacc caatactctc gaattgaatg gtctaacaga agaaacgcct
      241 catgacttac ccaagaatac cgctaagggc agagatgaag aagattttcc tctcgactat
      301 tttcctgtat ctgttgaata ccctacggag gaaaatgcgt ttgatccgtt ccctccacag
      361 gcttttacgc cagctgcccc ttccatgcct atttcctatg ataacgtgaa tgaaagggat
      421 tctatgcccg ttaattctct tcttaataga tacccctatc agttatcagt ggcacccact
      481 ttcccagtgc caccatcatc atcgaggcaa cattttatga caaatcggga tttttattca
      541 tctaacaata acaaggaaaa attggtatct cctagcgacc ctaccagcta catgaagtat
      601 gacgaaccag ttatggattt tgatgaatct cggccaaatg aaaactgtac aaatgcaaaa
      661 tctcacaact ctggccagca aactaaacaa caccaattat attctaacaa cttccagcaa
      721 tcttacccaa acggaatggt tccaggatac tacccaaaaa tgccgtataa tcccatgggg
      781 ggggatcctc tactcgatca agccttttat ggcgcggacg attttttctt tccaccagaa
      841 ggatgtgata acaatatgct gtatccacaa actgcaactt catggaatgt tttgccccct
      901 caagctatgc aaccagctcc aacctatgtt gggaggccat acacaccgaa ttatagatcg
      961 acaccaggtt ccgcgatgtt cccatacatg caaagttcaa attccatgca gtggaacact
     1021 gctgtttcac cttatagttc gagagcacca tctacaactg ctaaaaacta tcctcctagc
     1081 acattttatt ctcaaaatat aaatcaatac ccacggcgaa gaactgtggg aatgaagtca
     1141 tcacaaggaa atgttccaac aggtaataaa caatctgtgg gcaagtctgc aaaaatttca
     1201 aagcctctac atattaagac aagtgcttat cagaagcaat acaaaatcaa cttggaaacg
     1261 aaagccaggc caagtgctgg tgacgaagat tctgctcatc ctgataagaa caaagaaatt
     1321 tcgatgccta ctccggattc caatactttg gtggtccagt cagaagaagg tggagctcat
     1381 tcacttgagg tagataccaa tcgaaggtcc gataaaaacc ttccagatgc aaccggatcc
     1441 tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1501 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1561 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1621 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1681 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1741 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct
     1801 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1861 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1921 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1981 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     2041 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2101 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2161 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     2221 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     2281 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct
     2341 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2401 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2461 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2521 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2581 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2641 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2701 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2761 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2821 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2881 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2941 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3001 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3061 ttatcccctg attctgtgga taaccgt
//
