LOCUS       Exported                3759 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Regulator of G protein signalling.
ACCESSION   .
VERSION     .
KEYWORDS    pWS081
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      2126..2160
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2231..2890)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2805..2824
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3072..3660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3561..3580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatggttga caagaacagg acattgcatg aattgtcatc taagaatttc
       61 agtaggacgc caaatggtct tatctttaca aatgatctaa aaaccgtata ttcaatattt
      121 ttgatttgcc ttgacctaaa ggaaaagaaa cacagttcag atactaaatc ctttctacta
      181 acagctttca ccaaacattt ccattttaca ttcacgtatc aggaggcaat caaggccatg
      241 ggtcaactgg aactaaaagt ggatatgaat actacgtgca tcaatgtgag ttataatatt
      301 aagccgagct tagcaagaca tctactaacc ctattcatga gttcaaagtt attacatacg
      361 cctcaagatc gtacaagagg tgagcccaag gagaaggtat tattccaacc tacgcccaaa
      421 ggtgtcgcgg ttctgcagaa atacgtcaga gacattggtc taaagaccat gccggacata
      481 ttgctgtcta gtttcaactc tatgaagctg tttacattcg agcgttcttc cgttactgac
      541 agcattattc attcagacta tctgatacat atcctattca ttaaaatgat gggtgctaag
      601 cccaacgttt ggagcccaac caatgccgac gaccctctgc catgcctgag tagtttatta
      661 gagtatacga ataacgacga cacatttacc ttcgaaaagt ctaaaccgga gcagggatgg
      721 caagctcaaa tcggaaacat agacatcaat gatctagaga gagtctcccc gcttgcccac
      781 aggtttttta cgaaccctga tagtgaatcc cacactcaat attatgtaag taatgctggt
      841 ataagacttt tcgaaaataa gacgttcggc acgtcaaaga agatagtgat taaatacacg
      901 tttacgacaa aagcaatttg gcaatggatc atggactgca cagatataat gcatgtaaaa
      961 gaggcagtgt ccctagccgc acttttcttg aaaacaggat tgatcgtacc cgtactattg
     1021 caaccaagcc gtacggataa gaaaaaattt caaatcagta ggtctagctt cttcaccctt
     1081 agcaaacgtg gctgggatct agtatcctgg accgggtgca agtctaacaa tattcgtgca
     1141 ccaaacggct ccacgataga cctggacttt accctgagag gccatatgac agttagggac
     1201 gagaaaaaga ctctagatga ttctgaaggc tttagccaag atatgttgat ctcaagcagc
     1261 aatcttaaca aattagacta cgtgctaact gatcctggca tgcgttactt gttccgtaga
     1321 catctggaga aagagctttg cgtggagaat ttagatgttt tcattgagat aaaacgtttc
     1381 ctaaagaaga tgaccatttt aaaaaaactg atcgattcaa agcactgcga taaaaagtcc
     1441 aatacgagca caagtaagaa taacatcgtg aaaaccatcg atagtgcctt aatgaagcag
     1501 gccaacgagt gtttggaaat ggcctatcac atctattcct cttatataat gattggaagt
     1561 ccatatcaat tgaacattca tcacaacttg agacagaaca tttctgacat tatgttacac
     1621 ccacatagtc ctttgtcaga acactttcca acgaatctgt acgatccatc ccccgccagc
     1681 gccgagtccg ctgcatcttc aatttctagt acagaggcgg acacccttgg cgagccacca
     1741 gaagtgagcc tgaaaccaag taagaacctg agcaatgaaa attgctcatt caagaagcag
     1801 ggtttcaaac atcagttgaa agaatataag cctgccccct taacgctggc ggagactcat
     1861 tcccctaacg ctagtgtgga aaacagccat acgatcgtga gatatggtat ggacaatacg
     1921 cagaacgata cgaagtcagt ggaatcattt cctgctaccc ttaaagtatt aaggaagctt
     1981 taccccttat ttgaaatagt gtcaaatgag atgtataggt tgatgaacaa cgactccttc
     2041 caaaaattta cccagagtga tgtttacaag gacgcttctg ccttgattga aatacaggaa
     2101 aaatgcggat cctgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2161 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2221 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     2281 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     2341 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     2401 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga
     2461 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2521 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2581 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2641 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     2701 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     2761 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2821 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     2881 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     2941 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg
     3001 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3061 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3721 ttctttcctg cgttatcccc tgattctgtg gataaccgt
//
