LOCUS Exported 3367 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION G___ bicistronic protein. ACCESSION . VERSION . KEYWORDS pWS627 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3367) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 3367) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3367 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1315..1380 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /label=F2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" terminator 1734..1768 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(1839..2498) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2413..2432 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 2680..3268 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3169..3188 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc atatggcagc acatcagatg gactcgataa cgtattctaa taatgtcacc 61 caacagtata tacaaccaca aagtctacag gatatctctg cagtggagga tgaaattcaa 121 aataaaatag aggccgccag acaagagagt aaacagcttc atgctcaaat aaataaagca 181 aaacacaaga tacaagatgc aagcttattc cagatggcca acaaagttac ttcgttgacc 241 aaaaataaga tcaacttaaa gccaaatatc gtgttgaaag gccataataa taaaatctca 301 gattttcggt ggagtcgaga ttcaaaacgt attttgagtg caagtcaaga tggctttatg 361 cttatatggg acagtgcttc aggtttaaaa cagaacgcta ttccattaga ttctcaatgg 421 gttctttcct gcgctatttc gccatcgagt actttggtag caagcgcagg attaaacaat 481 aactgtacca tttatagagt ttcgaaagaa aacagagtag cgcaaaacgt tgcgtcaatt 541 ttcaaaggac atacttgcta tatttctgac attgaattta cagataacgc acatatattg 601 acagcaagtg gggatatgac atgtgccttg tgggatatac cgaaagcaaa gagggtgaga 661 gaatattctg accatttagg tgatgttttg gcattagcta ttcctgaaga gccaaactca 721 gaaaattctt cgaacacatt cgctagctgt ggatcagacg ggtatactta catatgggat 781 agcagatctc cgtccgctgt acaaagcttt tacgttaacg atagtgatat taatgcactt 841 cgttttttca aagacgggat gtcgattgtt gcaggaagtg acaatggtgc gataaatatg 901 tatgatttaa ggtcggactg ttctattgct actttttctc tttttcgagg ttatgaagaa 961 cgtaccccta cccctactta tatggcagct aacatggagt acaataccgc gcaatcgcca 1021 caaactttaa aatcaacaag ctcaagctat ctagacaacc aaggcgttgt ttctttagat 1081 tttagtgcat ctggaagatt gatgtactca tgctatacag acattggttg tgttgtgtgg 1141 gatgtattaa aaggagagat tgttggaaaa ttagaaggtc atggtggcag agtcactggt 1201 gtgcgctcga gtccagatgg gttagctgta tgtacaggtt catgggactc aaccatgaaa 1261 atatggtcac caggttatca agaagctaga cataaacaaa agattgttgc tccagttaaa 1321 caaactttga actttgattt gttgaaattg gctggtgatg ttgaatctaa tccagggccc 1381 acatcagttc aaaactctcc acgcttacaa caacctcagg aacagcaaca gcaacagcaa 1441 cagctttcct taaagataaa acaattgaag ttaaaaagaa tcaacgaact taacaataaa 1501 ctgaggaaag aactcagccg tgaaagaatt actgcttcaa atgcatgtct tacaataata 1561 aactatacct cgaatacaaa agattataca ttaccagaac tatggggcta ccccgtagca 1621 ggatcaaatc attttataga gggtttgaaa aatgctcaaa aaaatagcca aatgtcaaac 1681 tcaaatagtg tttgttgtac gcttatgtaa ctcgagtggc tgagaccaga ccaataaaaa 1741 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg 1801 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca 1861 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 1921 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 1981 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 2041 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa 2101 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 2161 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 2221 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga 2281 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 2341 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 2401 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 2461 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 2521 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 2581 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca 2641 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 2701 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 2761 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 2821 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 2881 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 2941 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 3001 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 3061 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 3121 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 3181 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 3241 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 3301 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 3361 taaccgt //