LOCUS       Exported               10063 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Open reading frame vector encoding Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pLX307 Luciferase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             66..110
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        341..551
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        363..559
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      410..545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             591..1193
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(591..610)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1087..1107
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1236..1353
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1491..2669
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1722..2660
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2617..2637
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2689..2713
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2716..4365
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2782..2800)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(4367..4391)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4399..4440
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4491..5079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4544..4564)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4962..4973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5151..5384
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5462..5583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5499..5518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5553..5572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5623..5758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5778..5797)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5779..5797)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5807..5824)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5807..5823)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5816..5838)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5965..6420
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6052..6071)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6262..6283
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6446..6550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6551..7411
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6769..6788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7582..8170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8071..8090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8324..8341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8458..8479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8494..8524
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8532..8548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8537..8559
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8556..8572
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8556..8572
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8591..8611
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8593..8611
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8639..8865
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8866..9046
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9093..9218
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9711..9944
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
       61 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      121 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
      181 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
      241 caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa
      301 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      361 catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      421 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      481 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      541 aggcctaggc ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt
      601 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
      661 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
      721 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
      781 tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag
      841 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
      901 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
      961 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     1021 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     1081 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     1141 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc
     1201 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     1261 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     1321 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     1381 ccactttggc gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga
     1441 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
     1501 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     1561 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     1621 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     1681 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     1741 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
     1801 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     1861 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     1921 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     1981 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     2041 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     2101 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     2161 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     2221 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     2281 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     2341 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     2401 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     2461 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     2521 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     2581 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     2641 gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac aagtttgtac
     2701 aaaaaagttg gcaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     2761 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     2821 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     2881 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     2941 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     3001 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     3061 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     3121 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     3181 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     3241 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     3301 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     3361 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     3421 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     3481 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     3541 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     3601 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     3661 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     3721 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     3781 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     3841 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     3901 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     3961 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     4021 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     4081 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     4141 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     4201 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     4261 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     4321 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgttgcc aactttcttg
     4381 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     4441 tagtaatgaa ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac aatcaacctc
     4501 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4561 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4621 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4681 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4741 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4801 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4861 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4921 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4981 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5041 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     5101 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5161 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5221 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5281 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5341 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     5401 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5461 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5581 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     5641 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5701 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5761 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5821 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5881 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5941 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6001 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6061 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6121 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6181 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6241 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6301 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6361 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8341 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8401 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8461 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8521 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     8581 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     8641 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     8701 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     8761 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     8821 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     8881 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8941 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9001 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     9061 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     9121 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     9181 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     9241 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     9301 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     9361 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     9421 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     9481 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     9541 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9601 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     9661 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     9721 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     9781 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     9841 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     9901 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     9961 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    10021 ctggaacaga tttggaatca cacgacctgg atggagtggg aca
//
