LOCUS       Exported                8983 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Open reading frame vector encoding HRAS.
ACCESSION   .
VERSION     .
KEYWORDS    pLX307 HRAS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     390..410
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    539..656
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        794..1972
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1025..1963
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1920..1940
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1992..2016
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2019..2588
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(2590..2614)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2622..2663
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2714..3302
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2767..2787)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3185..3196)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3374..3607
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3685..3806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3722..3741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3776..3795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3846..3981
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4001..4020)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4002..4020)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4030..4047)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4030..4046)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4039..4061)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4188..4643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4275..4294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4485..4506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4669..4773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4774..5634
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4992..5011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5805..6393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6294..6313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6547..6564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6681..6702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6717..6747
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6755..6771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6760..6782
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6779..6795
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6779..6795
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6814..6834
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6816..6834
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6862..7088
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7089..7269
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7316..7441
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7934..8167
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8352..8396
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8627..8837
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8649..8845
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8696..8831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8877..496
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8877..8896)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
       61 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
      121 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
      181 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
      241 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
      301 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
      361 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
      421 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
      481 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
      541 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
      601 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
      661 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
      721 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
      781 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
      841 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
      901 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      961 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     1021 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     1081 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     1141 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     1201 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     1261 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     1321 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     1381 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     1441 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     1501 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     1561 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     1621 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     1681 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     1741 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     1801 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     1861 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     1921 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggctagca
     1981 tcgattgatc aacaagtttg tacaaaaaag ttggcaccat gacggaatat aagctggtgg
     2041 tggtgggcgc cgtcggtgtg ggcaagagtg cgctgaccat ccagctgatc cagaaccatt
     2101 ttgtggacga atacgacccc actatagagg attcctaccg gaagcaggtg gtcattgatg
     2161 gggagacgtg cctgttggac atcctggata ccgccggcca ggaggagtac agcgccatgc
     2221 gggaccagta catgcgcacc ggggagggct tcctgtgtgt gtttgccatc aacaacacca
     2281 agtcttttga ggacatccac cagtacaggg agcagatcaa acgggtgaag gactcggatg
     2341 acgtgcccat ggtgctggtg gggaacaagt gtgacctggc tgcacgcact gtggaatctc
     2401 ggcaggctca ggacctcgcc cgaagctacg gcatccccta catcgagacc tcggccaaga
     2461 cccggcaggg agtggaggat gccttctaca cgttggtgcg tgagatccgg cagcacaagc
     2521 tgcggaagct gaaccctcct gatgagagtg gccccggctg catgagctgc aagtgtgtgc
     2581 tctcctgatt gccaactttc ttgtacaaag tggttgatat cggtaagcct atccctaacc
     2641 ctctcctcgg tctcgattct acgtagtaat gaactagtac cggttaagtc gacaatcaac
     2701 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2761 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2821 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2881 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2941 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3001 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3061 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     3121 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3181 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3241 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3301 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     3361 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     3421 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3481 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3541 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3601 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     3661 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     3721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3781 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     3841 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3901 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3961 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     4021 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4081 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4141 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     4201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4501 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4561 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4621 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     4681 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4981 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5281 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6601 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6661 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6721 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6781 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     6841 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     6901 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     6961 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     7021 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     7081 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7141 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7201 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7261 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     7321 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     7381 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     7441 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     7501 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     7561 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     7621 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     7681 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     7741 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     7801 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     7861 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     7921 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     7981 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     8041 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     8101 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     8161 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     8221 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     8281 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     8341 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     8401 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     8461 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     8521 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca
     8581 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8641 gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8701 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8761 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8821 ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga
     8881 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     8941 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca cac
//
