LOCUS       Exported               13926 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with
            increased virulence in Agrobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pSB90 - pAGM4723_VirGN54D
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13926)
  AUTHORS   Mortensen S., Bernal-Franco D., Cole LF., Sathitloetsakun S., Cram 
            EJ., Lee-Parsons CW.
  TITLE     EASI transformation: An efficient transient expression method for 
            analyzing gene function in Catharanthus roseus seedlings
  JOURNAL   frontiers in Plant Science, 2019
REFERENCE   2  (bases 1 to 13926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13926
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      6118..6127
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    8132..8156
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8436..9065
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9499..10566
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10632..10826
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        10888..10992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10993..11787
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13111..13699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13600..13619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    13835..13859
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccttgtc ttccagtggt atggggtacc gcacggtctg ccaatccgac ggaggtttat
       61 gaattttcca ccttttccac aagctcaact agtattaacg atgtggattt agcaaaaaaa
      121 acctgtaacc ctaaatgtaa aataacgggt aagcctgcca accatgttat ggcagattaa
      181 gcgtcttttt gaagggcacc gcatctttcg cgttgccgta aatgtatccg tttagcaata
      241 aggaaattct taatgaataa tccgtcgtta ctcaatcatg cggtcgaaac gatggcagtt
      301 ggctcgaaaa gttttgcgac agcctcaaag ttatttgatg caaaaacccg gcgcagcgta
      361 ctgatgctct acgcctggtg ccgccattgt gacgatgtta ttgacgatca gacgctgggc
      421 tttcaggccc ggcagcctgc cttacaaacg cccgaacaac gtctgatgca acttgagatg
      481 aaaacgcgcc aggcctatgc aggatcgcag atgcacgaac cggcgtttgc ggcttttcgg
      541 gaagtggcta tggctcatga tatcgccccg gcttacgcgt ttgatcatct ggaaggcttc
      601 gccatggatg tacgcgaagc gcaatacagc caactggatg atacgctgcg ctattgctat
      661 cacgttgcag gcgttgtcgg cttgatgatg gcgcaaatca tgggcgtgcg ggataacgcc
      721 acgctggacc gcgcctgtga ccttgggctg gcatttcagt tgaccaatat tgctcgcgat
      781 attgtggacg atgcgcatgc gggccgctgt tatctgccgg caagctggct ggagcatgaa
      841 ggtctgaaca aagagaatta tgcggcacct gaaaaccgtc aggcgctgag ccgtatcgcc
      901 cgtcgtttgg tgcaggaagc agaaccttac tatttgtctg ccacagccgg cctggcaggg
      961 ttgcccctgc gttccgcctg ggcaatcgct acggcgaagc aggtttaccg gaaaataggt
     1021 gtcaaagttg aacaggccgg tcagcaagcc tgggatcagc ggcagtcaac gaccacgccc
     1081 gaaaaattaa cgctgctgct ggccgcctct ggtcaggccc ttacttcccg gatgcgggct
     1141 catcctcccc gccctgcgca tctctggcag cgcccgctct aggcttacta nvaggadnnn
     1201 nnnaatgaaa ccaactacgg taattggtgc aggcttcggt ggcctggcac tggcaattcg
     1261 tctacaagct gcggggatcc ccgtcttact gcttgaacaa cgtgataaac ccggcggtcg
     1321 ggcttatgtc tacgaggatc aggggtttac ctttgatgca ggcccgacgg ttatcaccga
     1381 tcccagtgcc attgaagaac tgtttgcact ggcaggaaaa cagttaaaag agtatgtcga
     1441 actgctgccg gttacgccgt tttaccgcct gtgttgggag tcagggaagg tctttaatta
     1501 cgataacgat caaacccggc tcgaagcgca gattcagcag tttaatcccc gcgatgtcga
     1561 aggttatcgt cagtttctgg actattcacg cgcggtgttt aaagaaggct atctaaagct
     1621 cggtactgtc ccttttttat cgttcagaga catgcttcgc gccgcacctc aactggcgaa
     1681 actgcaggca tggagaagcg tttacagtaa ggttgccagt tacatcgaag atgaacatct
     1741 gcgccaggcg ttttctttcc actcgctgtt ggtgggcggc aatcccttcg ccacctcatc
     1801 catttatacg ttgatacacg cgctggagcg tgagtggggc gtctggtttc cgcgtggcgg
     1861 caccggcgca ttagttcagg ggatgataaa gctgtttcag gatctgggtg gcgaagtcgt
     1921 gttaaacgcc agagtcagcc atatggaaac gacaggaaac aagattgaag ccgtgcattt
     1981 agaggacggt cgcaggttcc tgacgcaagc cgtcgcgtca aatgcagatg tggttcatac
     2041 ctatcgcgac ctgttaagcc agcaccctgc cgcggttaag cagtccaaca aactgcagac
     2101 taagcgcatg agtaactctc tgtttgtgct ctattttggt ttgaatcacc atcatgatca
     2161 gctcgcgcat cacacggttt gtttcggccc gcgttaccgc gagctgattg acgaaatttt
     2221 taatcatgat ggcctcgcag aggacttctc actttatctg cacgcgccct gtgtcacgga
     2281 ttcgtcactg gcgcctgaag gttgcggcag ttactatgtg ttggcgccgg tgccgcattt
     2341 aggcaccgcg aacctcgact ggacggttga ggggccaaaa ctacgcgacc gtatttttgc
     2401 gtaccttgag cagcattaca tgcctggctt acggagtcag ctggtcacgc accggatgtt
     2461 tacgccgttt gattttcgcg accagcttaa tgcctatcat ggctcagcct tttctgtgga
     2521 gcccgttctt acccagagcg cctggtttcg gccgcataac cgcgataaaa ccattactaa
     2581 tctctacctg gtcggcgcag gcacgcatcc cggcgcaggc attcctggcg tcatcggctc
     2641 ggcaaaagcg acagcaggtt tgatgctgga ggatctgata tgagctttta cnvaggadnn
     2701 nnnnaatgca accgcattat gatctgattc tcgtgggggc tggactcgcg aatggcctta
     2761 tcgccctgcg acttcagcag cagcaacctg atatgcgtat tttgcttatc gacgccgcac
     2821 cccaggcggg cgggaatcat acgtggtcat ttcaccacga tgatttgact gagagccaac
     2881 atcgttggat agctccgctg gtggttcatc actggcccga ctatcaggta cgctttccca
     2941 cacgccgtcg taagctgaac agcggctact tttgtattac ttctcagcgt ttcgctgagg
     3001 ttttacagcg acagtttggc ccgcacttgt ggatggatac cgcggtcgca gaggttaatg
     3061 cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc
     3121 ggggttatgc ggcaaattca gcactgagcg tgggcttcca ggcgtttatt ggccaggaat
     3181 ggcgattgag tcacccgcat ggtttatcat ctccgattat catggatgcc acggtcgatc
     3241 agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc gccgaccaga ttgttaattg
     3301 aagatacgca ctatattgat aatgcgacat tagatcctga atgcgcgcgg caaaatattt
     3361 gcgactatgc cgcgcaacag ggttggcagc ttcagacact gctgcgagaa gaacagggcg
     3421 ccttacccat tactctgtcg ggcaatgccg acgcattctg gcagcagcgc cccctggcct
     3481 gtagtggatt acgtgccggt ctgttccatc ctaccaccgg ctattcactg ccgctggcgg
     3541 ttgccgtggc cgaccgcctg agtgcacttg atgtctttac gtcggcctca attcaccatg
     3601 ccattacgca ttttgcccgc gagcgctggc agcagcaggg ctttttccgc atgctgaatc
     3661 gcatgctgtt tttagccgga cccgccgatt cacgctggcg ggttatgcag cgtttttatg
     3721 gtttacctga agatttaatt gcccgttttt atgcgggaaa actcacgctg accgatcggc
     3781 tacgtattct gagcggcaag ccgcctgttc cggtattagc agcattgcaa gccattatga
     3841 cgactcatcg ttaagcttca ganvaggadn nnnnnaatga gttttgatat tgccaaatac
     3901 ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa agagagttta
     3961 ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg ttccagcggg
     4021 cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta tgtctacaac
     4081 accccgtttg accaattgat ttgggatgtg gggcatcagg cttatccgca taaaattttg
     4141 accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca cccgttcccg
     4201 tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac ctccattagt
     4261 gccggaattg gtattgcggt tgctgccgaa aaagaaggca aaaatcgccg caccgtctgt
     4321 gtcattggcg atggcgcgat taccgcaggc atggcgtttg aagcgatgaa tcacgcgggc
     4381 gatatccgtc ctgatatgct ggtgattctc aacgacaatg aaatgtcgat ttccgaaaat
     4441 gtcggcgcgc tcaacaacca tctggcacag ctgctttccg gtaagcttta ctcttcactg
     4501 cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccaa ttaaagagct gctcaaacgc
     4561 accgaagaac atattaaagg catggtagtg cctggcacgt tgtttgagga gctgggcttt
     4621 aactacatcg gcccggtgga cggtcatgat gtgctggggc ttatcaccac gctaaagaac
     4681 atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg tcgtggttat
     4741 gaaccggcag aaaaagaccc gatcactttc catgccgtgc ctaaatttga tccctccagc
     4801 ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt tggcgactgg
     4861 ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc gatgcgtgaa
     4921 ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gctacttcga cgtggcaatt
     4981 gccgagcaac atgcggtgac ctttgctgcg ggtctggcga ttggtgggta caaacccatt
     5041 gtcgcgattt actccacttt cctgcaacgc gcctatgatc aggtgctgca tgacgtggcg
     5101 attcaaaagc ttccggtcct gttcgccatc gaccgcgcgg gcattgttgg tgctgacggt
     5161 caaacccatc agggtgcttt tgatctctct tacctgcgct gcataccgga aatggtcatt
     5221 atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta tcactataac
     5281 gatggcccgt cagcggtgcg ctacccgcgt ggcaacgcgg tcggcgtgga actgacgccg
     5341 ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa actggcgatc
     5401 cttaactttg gtacgctgat gccagaagcg gcgaaagtcg ccgaatcgct gaacgccacg
     5461 ctggtcgata tgcgttttgt gaaaccgctt gatgaagcgt taattctgga aatggccgcc
     5521 agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc aggcagcggc
     5581 gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat tggcctgccg
     5641 gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg cctcgatgcc
     5701 gctggtatgg aagccaaaat caaggcctgg ctggcataag ctttgtgnva ggadnnnnnn
     5761 aatgagcgca catgccctgc ccaaggcaga tctgaccgcc accagcctga tcgtatcggg
     5821 cggcatcatc gccgcttggc tggccctgca tgtgcatgcg ctgtggtttc tggacgcagc
     5881 ggcgcatccc atcctggcga tcgcaaattt cctggggctg acctggctgt cggtcggatt
     5941 gttcatcatc gcgcatgacg cgatgcacgg gtcggtggtg ccggggcgtc cgcgcgccaa
     6001 tgcggcgatg ggccagcttg tcctgtggct gtatgccgga ttttcgtggc gcaagatgat
     6061 cgtcaagcac atggcccatc accgccatgc cggaaccgac gacgaccccg atttcgacca
     6121 tggcggcccg gtccgctggt acgcccgctt catcggcacc tatttcggct ggcgcgaggg
     6181 gctgcttctg cccgtcatcg tgacggtcta tgcgctgatc cttggggatc gctggatgta
     6241 cgtggtattc tggccgctgc cgtcgatcct ggcgtcgatc cagctgttcg tgttcggcac
     6301 ctggctgccg caccgccccg gccacgacgc gttcccggac cgccacaatg cgcggtcgtc
     6361 gcggatcagc gaccccgtgt cgctgctgac ctgttttcac tttggcggtt atcatcacga
     6421 acaccacctg cacccgacgg tgccgtggtg gcgcctgccc agcacccgca ccaagggcga
     6481 caccgcataa gcttgagcnv aggadnnnnn naatgcaaac ggaacacgtc attttattga
     6541 atgcacaggg agttcccacg ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc
     6601 gcttacatct cgcgttctcc agttggctgt ttaatgccaa aggacaatta ttagttaccc
     6661 gccgcgcact gagcaaaaaa gcatggcctg gcgtgtggac taactcggtt tgtgggcacc
     6721 cacaactggg agaaagcaac gaggacgcag tgatccgccg ttgccgttat gagcttggcg
     6781 tggaaattac gcctcctgaa tctatctatc ctgactttcg ctaccgcgcc accgatccga
     6841 gtggcattgt ggaaaatgaa gtgtgtccgg tatttgccgc acgcaccact agtgcgttac
     6901 agatcaatga tgatgaagtg atggattatc aatggtgtga tttagcagat gtattacacg
     6961 gtattgatgc aacgccgtgg gcgttcagtc cgtggatggt gatgcaggcg acaaatcgcg
     7021 aagccagaaa acgattatct gcatttaccc agcttaaata agctttgccn vaggadnnnn
     7081 nnaatgacgg tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc ggagcagtta
     7141 ctggctgata ttgatcgacg ccttgatcag ttattgcccg tggagggaga acgggatgtt
     7201 gtgggtgccg cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg ccccatgttg
     7261 ctgttgctga ccgcccgcga tctgggttgc gctgtcagcc atgacggatt actggatttg
     7321 gcctgtgcgg tggaaatggt ccacgcggct tcgctgatcc ttgacgatat gccctgcatg
     7381 gacgatgcga agctgcggcg cggacgccct accattcatt ctcattacgg agagcatgtg
     7441 gcaatactgg cggcggttgc cttgctgagt aaagcctttg gcgtaattgc cgatgcagat
     7501 ggcctcacgc cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc catcggcatg
     7561 caaggattgg ttcagggtca gttcaaggat ctgtctgaag gggataagcc gcgcagcgct
     7621 gaagctattt tgatgacgaa tcactttaaa accagcacgc tgttttgtgc ctcgatgcag
     7681 atggcctcga ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca tcgtttttca
     7741 cttgatcttg gtcaggcatt tcaactgctg gacgatttga ccgatggcat gaccgacacc
     7801 ggtaaggata gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt aggcccgagg
     7861 gcggttgaag aacgtctgag acaacatctt cagcttgcca gtgagcatct ctctgcggcc
     7921 tgccaacacg ggcacgccac tcaacatttt attcaggcct ggtttgacaa aaaactcgct
     7981 gccgtcagtt aagcttgcaa gaattcaagc ttggagtacg ttaattagtt gtctgtttta
     8041 agccccgtgc tctccgccgg ggctttttta ttaaaaacca ctcaagtgga agacaaggga
     8101 cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     8161 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     8221 ttcgtccatt tgtatgtgcc agccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     8281 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     8341 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     8401 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     8461 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     8521 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     8581 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     8641 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     8701 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     8761 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     8821 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     8881 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     8941 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     9001 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     9061 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     9121 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     9181 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     9241 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     9301 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     9361 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     9421 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     9481 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     9541 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     9601 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     9661 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     9721 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     9781 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     9841 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     9901 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     9961 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
    10021 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
    10081 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
    10141 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
    10201 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
    10261 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
    10321 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
    10381 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
    10441 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
    10501 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
    10561 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
    10621 agcttctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
    10681 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
    10741 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
    10801 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga acagctgcaa
    10861 aaagcgccca catcaaggct ccgagtgcgc ggaaccccta tttgtttatt tttctaaata
    10921 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10981 aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
    11041 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
    11101 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
    11161 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
    11221 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
    11281 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
    11341 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
    11401 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
    11461 gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
    11521 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
    11581 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
    11641 aagtggtatg acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg ggaagaacag
    11701 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
    11761 tatattttac tggatgaatt gttttagctg tcagaccaag tttactcata tatactttag
    11821 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11881 ctcatgacca aaatccctta acgtgagttt tcgttctagc tgtaacctcg aagcgtttca
    11941 cttgtaacaa cgattgagaa ttttgtcata aaattgaaat acttggttcg cattttcgtc
    12001 atccgcggtc agccgcaatt ctgacgaact gcccatttag ctggagatga ttgtacatcc
    12061 ttcacgtgaa aatttctcaa gcgctgtgaa caagggttca gattttagat tgaaaggtga
    12121 gccgttgaaa cacgttcttc ttgtcgatga cgacgtcgct atgcggcatc ttattattga
    12181 ataccttacg atccacgcct tcaaagtgac cgcggtagcc gacagcaccc agttcacaag
    12241 agtactctct tccgcgacgg tcgatgtcgt ggttgttgat ctagatttag gtcgtgaaga
    12301 tgggctcgag atcgttcgta atctggcggc aaagtctgat attccaatca taattatcag
    12361 tggcgaccgc cttgaggaga cggataaagt tgttgcactc gagctaggag caagtgattt
    12421 tatcgctaag ccgttcagta tcagagagtt tctagcacgc attcgggttg ccttgcgcgt
    12481 gcgccccaac gttgtccgct ccaaagaccg acggtctttt tgttttactg actggacact
    12541 taatctcagg caacgtcgct tgatgtccga agctggcggt gaggtgaaac ttacggcagg
    12601 tgagttcaat cttctcctcg cgtttttaga gaaaccccgc gacgttctat cgcgcgagca
    12661 acttctcatt gccagtcgag tacgcgacga ggaggtttat gacaggagta tagatgttct
    12721 cattttgagg ctgcgccgca aacttgaggc ggatccgtca agccctcaac tgataaaaac
    12781 agcaagaggt gccggttatt tctttgacgc ggacgtgcag gtttcgcacg gggggacgat
    12841 ggcagcctga gccaattgca tttgcctctt aattatctgg ctcaaagggt gactgaggag
    12901 taagcgatgt gcccatcaca ctgaccacca agacgggggt tcgttttgaa gatcaccctt
    12961 cagacccaag gccagtgtgc gttgatcgcg gccacactgc aaattgacat actgtcgata
    13021 aaggggcgtt ggacgagaac ccattcgtca ggatactggc tcgtgatctc actgagcgtc
    13081 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    13141 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    13201 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    13261 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    13321 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    13381 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    13441 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    13501 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    13561 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    13621 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    13681 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tgctcggatc
    13741 tgttggaccg gacagtagtc atggttgatg ggctgcctgt atcgagtggt gattttgtgc
    13801 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
    13861 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggttgaa
    13921 cactct
//
