LOCUS       Exported                3184 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  crtB in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    pStA0::crtB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      171..265
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      complement(423..1011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(503..522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1182..2042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1805..1824
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2043..2147)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2215..2233
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2246..2332
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtctttgagt gctggcgggt caaatcccga cagtagcgaa ccgcctgagc cagtggagga
       61 cggcgcagtt cggtgcaggg cagggcgggg gagtaatcgg tcacggtcaa ggtacgcgtg
      121 ggtaaccgta agctcagacc agcgcgacta cgcatcgaag agcacactgg attctcacca
      181 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      241 attactggat ctatcaacag gagtccaagg cgctcggtcg ttcggctgcg gcgagcggta
      301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      841 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      901 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      961 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1021 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1081 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1141 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1201 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1261 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1321 cgggacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1381 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1441 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1501 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1561 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1621 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1681 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1741 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1801 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1861 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1921 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1981 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2041 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2101 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2161 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2221 cgtatcacga ggccctttcg ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2281 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     2341 ctagaggtct tacgctcttc attagccgtg gacagcgccc agactacgca gttcccacca
     2401 ggcctgcggt aacatcagaa gtttacgacg acctgacacg tacgcgcgcc gaccgaagtt
     2461 gtcgtagccg gcccgttcca aatcgtccag aataccctca tacgcacggg cggcagtcag
     2521 aacggcgaga ggaccgcgtc catcaagttg aggaatacca gcacgacctg ctgcatacca
     2581 ttcacgtgca aggccgccaa gatgagtcat gagagcacgg taggccggtg acaggggctc
     2641 accctgaccc cagcgctcta acgcggcgcg agacaggcca tattcatcaa gcagagactg
     2701 tggcaggtat acgcggccgc gggtcagatc ttcaccgaca tcgcgcagaa tattcgtcag
     2761 ttgcatcgcc tgccctagtt ggagagcatc attcagggta gcagcccccc cacggtagcc
     2821 agaaatcggt gccaccataa agccaaccac acctgccaca cggcggcaat acagtaacaa
     2881 gtcatccata tcacggtatt catgaccgcg taaatccatg ttgaggcctt catgcagttc
     2941 cgcgaaagct gagtgcggga tggcgtaccg acctgccgcc caggccagcg cggttgagat
     3001 gggatcatcc gctggttggc cagcaaacgc ggcatcaata cggctgcgcc attcccgcaa
     3061 ttcgcgttcg cggtcgccgt tgccggcttc atcgacgatg tcatcgcccg cgcggcacgc
     3121 cgcatacact gcccaaactg ccgcgcgttc cggaggcgaa aagagctgtg aacccagata
     3181 gaag
//
