LOCUS       Exported               10397 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  neutrophil specific expression.
ACCESSION   .
VERSION     .
KEYWORDS    Tol2-lyzC-mcherry-2a-CDK2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(704..720)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(757..775)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(796..812)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(796..812)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(809..831)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    820..836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(844..874)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    889..910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1027..1044)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1198..1786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1278..1297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1957..2817)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2580..2599
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2818..2922)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2948..3403)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3085..3106)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3297..3316
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3530..3552
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3545..3561
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3571..3589
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4265..4285
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6688..6712
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     6714..6730
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6740..6758
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6814..6823
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6820..7527
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6965..6983)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7233..7252
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7537..7593
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(8506..8522)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8553..8571)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(8600..8624)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8629..8763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8653..8672)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8707..8726
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtaaaacaaa atacttaatg tacttacctc aatatgcttc cttaggtttg atggcgaact
       61 tttgaaggcc gatatttctt tattaagcgg gagacagagg acacatttca tcttgaatga
      121 atctttattt acaccactta aagaaaagaa ttcgcgaaga tacggccacg ggtgctcttg
      181 atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc
      241 cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc
      301 attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca
      361 atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg
      421 ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc
      481 caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt
      541 tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa
      601 gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt
      661 tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga
      721 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
      781 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      841 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      901 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      961 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1021 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1081 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1141 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1201 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1261 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1321 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1381 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1441 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1501 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1561 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1621 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1681 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1741 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1801 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1861 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1921 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1981 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2041 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2101 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2161 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2221 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2281 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2341 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2401 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2461 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2521 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2581 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2641 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2701 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2761 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2821 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2881 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2941 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     3001 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     3061 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     3121 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     3181 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     3241 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     3301 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     3361 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     3421 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     3481 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     3541 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     3601 acaggtcatt gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt
     3661 cagacaccca tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
     3721 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
     3781 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
     3841 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
     3901 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
     3961 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
     4021 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
     4081 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
     4141 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
     4201 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccg
     4261 atatcaactt tgtatagaaa agttggctcc gaattcgccc ttaaaactcg agagtttgac
     4321 ttgggttgat tatagcaaat agtggaacac aaacagattt tgaaatattc ttgcaaattt
     4381 atttattaaa tccattcaaa tattgtcatt ttttgtactg tgtaatttat attgatttgc
     4441 tcatacaata tttacatttc gcttaaacaa tacactatta tttatattta aaaatagcta
     4501 tatataaatc aaatgtatag ctctttttta cacaattaat caaagctaaa atatttgtca
     4561 cacaaataca gtaagccact tcacaatgat atcttcaaaa tctgtgcttg actgaatcat
     4621 ttcatttatt gttttgtaat tcacattata ccttacgttc tccaggcaca gtttgaattg
     4681 ttgataaatg aagataatac atctgattta attagtctcc tttgtttata ataactttaa
     4741 tctaatttga tagtaataat tccagcaata catccattac aataaaaatc gcttttttat
     4801 tttgcacatt agtttgccgg tatttgttta aagtttcacc atacttttaa atcatgtgct
     4861 atttgaaaag ggctgttatg actggaatta aatgtttttt tttatatcag tgttttagat
     4921 tgaagtacaa tatttatagg taaaattcac cagcatatgc ttaaagatag ccatgctcca
     4981 ggtccttgtg tccttctaag gtctgtttgt ggtttaagct gtttgttgac acacgcttgt
     5041 cttctccatt tttctgtgaa gttattgtca gtttattgca cactgggttg cattaagctg
     5101 cagttttttc acaacagttt tccacattgt aacagtcagt ctccaaatta ttccatcagt
     5161 cctcaaattt agtcatttga ggccatgaaa acacttaaaa ctacatgaag gtagtttggc
     5221 attaggtttt agacttgttc ttgtttggca tattgcaacc tgtgtaaaaa tgatttgatt
     5281 tttatttttt tttttttttt ttcagaaaga aacggtccaa aaagagttat gtgaacattt
     5341 atccatgtgt ttaatgtttt ttaaattaaa aaaaaacatt tttattaaac cacttgtgac
     5401 taaacctttt ttagttattc tattttgtga cgagtctgaa tggccaaagt actcttgaat
     5461 ccaaaatctt tatagtaaaa tgtttaatct tacttgcagt gttgggtata atttgtaaca
     5521 aagttgaatt ccatatctgc tgagtgaaaa agtaaagtaa gagattgctg taaatattta
     5581 ggttatttat ttattaatac aatatagaaa gtaacaaaac acaatgtaaa aaatcaataa
     5641 agaaagtaaa tattaaaagt aatgataaac taatgatgta aacaatatta gcaacaaagt
     5701 ttgtacaatt cagggagggt aaagaatttc acctaatagt caaactctgt attaaataat
     5761 ataaaatctt tgttcttagt ctgaagagga gactgtgaga gaaagcttac tggttgttaa
     5821 attagaaaat ttgtaacttg cagattaaaa tgatttaaat aattttataa aggccatgtt
     5881 tgaaacaaat gtaaaaacat aataaatgtt aagaatttca gatattgaga ttttacaatt
     5941 taaaaaaagc aataaagtaa tagcagagac ctacactctt aaagtaaatt acccaactct
     6001 gcttactagt aacttttgga atagttacta gtatccattg aacaaatact agaatctaat
     6061 taaattgtgt gagtgtccaa tgaaaagatt cttgtattta aaacagtacc agtatctata
     6121 tatgtacaaa atttagtgct agcaaaatgc aaataaatac tactgcatat aatggattgc
     6181 attgtaaatc gactcgccat aatacattta tttcctattt ttacttttaa caaaaaataa
     6241 gttcactgag tatttcatgt cattaaatcg cattaaacct cttgcatgct ttatgtacac
     6301 tagtgcagtt cgctaaatga ccaccggtgt cactaataac acgatcgttt ctgaattctg
     6361 cattaaacgc catttatacg ttgctcttat tagtaaacaa tttctccatc atgagctcga
     6421 ccagtattgc atatttcatc ctcttaaatc cagagttgtt tcttttaatc actttctctt
     6481 catgataaaa gcagtttttg gttttcagaa gtttacaaca aacccagtca gttattgcct
     6541 cagcaaacac tggagcaact gcacacttgt gaaaagcttc ttgcgaaaca caaacctttc
     6601 tctggactta taaataaagg cattcagcag ctccaccata cacctccact ggtcttgata
     6661 aagggatcct ttaagggcga attcgaccca agtttgtaca aaaaagcagg cttgtaaaac
     6721 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc
     6781 cctcgaggtc gactcagcag tgatacaggt accgccacca tggtgagcaa gggcgaggag
     6841 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     6901 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     6961 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     7021 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     7081 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     7141 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     7201 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     7261 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     7321 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     7381 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     7441 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     7501 accggcggca tggacgagct gtacaaggga tctggagcca cgaacttctc tctgttaaag
     7561 caagcaggag acgtggaaga aaaccccggt cctatggagt cttttcagaa agtggagaag
     7621 atcggagagg gaacatacgg agtggtttat aaagccaaaa ataaagttac aggagaaact
     7681 gtcgcgctga agaaaatccg attagatacg gaaactgaag gtgttcccag cactgccatt
     7741 cgtgagatct ctctgctaaa agagctcaat caccctaaca tagtcaagtt gcgtgatgtg
     7801 atacacactg aaaataaact ttacttggtg tttgagtttc ttcaccaaga cctgaagagg
     7861 tttatggact cgacgtctgt cagtggcata tccttgccac tcgtgaagag ttacctattc
     7921 cagctgctcc agggattggc cttctgtcac tcccatcgtg ttcttcacag agatcttaaa
     7981 ccccagaatc tcctcatcaa cgctcagggc gagatcaaac tggctgactt tggtttggcc
     8041 agagcgttcg gtgtacctgt gcggacttat acacatgagg ttgtaacttt gtggtacaga
     8101 gctccagaga ttctcctggg atgtaaatat tattctacag ctgttgacat ctggagtttg
     8161 ggctgcatct ttgctgaaat gattactcgg agggcactgt ttcctggaga ctctgaaata
     8221 gatcagctct tccggatatt tcgaactctg ggcactcctg atgagtctat atggcctgga
     8281 gttacatcaa tgccagacta caaaccctcc tttcccaaat gggctcgaca ggacctgtct
     8341 aaagtggtgc ctccccttga tgaagatggc agagaccttc tcgggcaaat gttgacctat
     8401 gatccaaaca agaggatctc ggcaaagaac gctcttgttc atcggttttt ccgcgacgtc
     8461 accatgccag tgcctccttt acgcctataa tcataatcag ccatggatcc actagttcta
     8521 gagcggccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     8581 gcgtaatcat ggtcatagca cccagctttc ttgtacaaag ttgatgtaga tccagacatg
     8641 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     8701 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     8761 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     8821 ttttaattcg cggccgcggc gccaatgcat tgggccctcg agcatatgag atctgtcccg
     8881 atctttctct tctgtgctgt caatttagag tagtttttga ggtaatttgg gtgcattctc
     8941 tgtaaaaaca acaaataaat atttatattg tatgcaataa atgtaacaat tacacataca
     9001 ctctctgatt acagcattaa agggttagtt cacccaaaaa tgaaaataat gtcattaatg
     9061 actcgccctc atgtcgttcc aagcccgtaa gacctccgtt catcttcaga acacagttta
     9121 agatatttta gatttagtcc gagagctttc tgtgcctcca ttgagaatgt atgtacggta
     9181 tactgtccat gtccagaaag gtaataaaaa catcaaagta gtccatgtga catcagtggg
     9241 ttagttagaa ttttttgaag catcgaatac attttggtcc aaaaataaca aaacctacga
     9301 ctttattcgg cattgtattc tcttccgggt ctgttgtcaa tccgcgttca cgacttcgca
     9361 gtgacgctac aatgctgaat aaagtcgtag gttttgttat ttttggacca aaatgtattt
     9421 tcgatgcttc aaataattct acctaaccca ctgatgtcac atggactact ttgatgtttt
     9481 tattaccttt ctggacatgg acagtatacc gtacatacat tttcagtgga gggacagaaa
     9541 gctctcggac taaatctaaa atatcttaaa ctgtgttccg aagatgaacg gaggtgttac
     9601 gggcttggaa cgacatgagg gtgagtcatt aatgacatct tttcattttt gggtgaacta
     9661 accctttaat aaaactgaca tttgcagcca attgcacact ttataatgtt aaatacttgt
     9721 tgattttaga aatacagtag catttagtga caatcaaaca ttaattttat ttctgattat
     9781 attgacaaaa acacaggact gtaaggctga catgacaggg tgaattgatt tcattacatt
     9841 agttttgatg gtaacacttt tacaataagg ttcgttagtt aactacatta gttaacatga
     9901 actaataatg aactgcacgt atacatgtta atttcaacat ttactaatac tttatatctt
     9961 gttaacatta gttaatgcac tgtgaactaa catgaacaaa caatgaacat ctgtatttct
    10021 attaactaac gttaacaaag attaacagat acagtaacaa atgtattgct catggttatt
    10081 taatgttagt taatacatga actaatcaac ctcattgtaa agtgttaccg ttttgacaat
    10141 acaacatgtt tggaaaaaaa tttctcgctt ggttttcttg tgttttgcat gaattcaaac
    10201 acaatgagag cacggaatga taatgattat tcaaagctgc aataggcgcc aaatttcccg
    10261 tggacctaac gttaccaatt acaaatgcat tttagcattt tattacgtga gccttatatt
    10321 gtcaatcgca tgcattagtg aaaataggcc tactacacat ataaaaggcg cgccatcaac
    10381 gtcaaaacat gcacacc
//
